m39_methylomonas_medium

CultureMech:002344 bacterial DEFINED LIQUID
Original name: M39 METHYLOMONAS MEDIUM

Overview

External ontology
mediadive.medium:J1171 (source link unavailable) — M39 METHYLOMONAS MEDIUM
Applications
  • Microbial cultivation
pH
6.8
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1171

Composition

flowchart LR
    CultureMech_002344["m39_methylomonas_medium"]
    ing0_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_002344 --> ing0_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing0_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing1_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_002344 --> ing1_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing1_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing2_K2SO4["K2SO4"]
    CultureMech_002344 --> ing2_K2SO4
    CHEBI_32036["CHEBI:32036"]
    ing2_K2SO4 -.-> CHEBI_32036
    ing3_NaNO3["NaNO3"]
    CultureMech_002344 --> ing3_NaNO3
    CHEBI_63005["CHEBI:63005"]
    ing3_NaNO3 -.-> CHEBI_63005
    ing4_KH2PO4["KH2PO4"]
    CultureMech_002344 --> ing4_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing4_KH2PO4 -.-> CHEBI_63036
    ing5_CeCl3["CeCl3"]
    CultureMech_002344 --> ing5_CeCl3
    CHEBI_35458["CHEBI:35458"]
    ing5_CeCl3 -.-> CHEBI_35458
    ing6_NaHCO3["NaHCO3"]
    CultureMech_002344 --> ing6_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing6_NaHCO3 -.-> CHEBI_32139
    ing7_Na2_EDTA["Na2-EDTA"]
    CultureMech_002344 --> ing7_Na2_EDTA
    CHEBI_64734["CHEBI:64734"]
    ing7_Na2_EDTA -.-> CHEBI_64734
    ing8_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
    CultureMech_002344 --> ing8_FeCl2_x_4_H2O
    CHEBI_86249["CHEBI:86249"]
    ing8_FeCl2_x_4_H2O -.-> CHEBI_86249
    ing9_Biotin["Biotin"]
    CultureMech_002344 --> ing9_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing9_Biotin -.-> CHEBI_15956
    ing10_Folic_acid["Folic acid"]
    CultureMech_002344 --> ing10_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing10_Folic_acid -.-> CHEBI_27470
    ing11_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
    CultureMech_002344 --> ing11_Pyridoxine_hydrochloride
    CHEBI_30961["CHEBI:30961"]
    ing11_Pyridoxine_hydrochloride -.-> CHEBI_30961
    ing12_Thiamine_HCl["Thiamine HCl"]
    CultureMech_002344 --> ing12_Thiamine_HCl
    CHEBI_49105["CHEBI:49105"]
    ing12_Thiamine_HCl -.-> CHEBI_49105
    ing13_Riboflavin["Riboflavin"]
    CultureMech_002344 --> ing13_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing13_Riboflavin -.-> CHEBI_17015
    ing14_Nicotinic_acid["Nicotinic acid"]
    CultureMech_002344 --> ing14_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing14_Nicotinic_acid -.-> CHEBI_15940
    ing15_Calcium_pantothenate["Calcium pantothenate"]
    CultureMech_002344 --> ing15_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing15_Calcium_pantothenate -.-> CHEBI_31345
    ing16_Vitamin_B12["Vitamin B12"]
    CultureMech_002344 --> ing16_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing16_Vitamin_B12 -.-> CHEBI_176843
    ing17_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_002344 --> ing17_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing17_p_Aminobenzoic_acid -.-> CHEBI_30753
    ing18_Lipoic_acid["Lipoic acid"]
    CultureMech_002344 --> ing18_Lipoic_acid
    CHEBI_16494["CHEBI:16494"]
    ing18_Lipoic_acid -.-> CHEBI_16494
    ing19_1_4_Naphthoquinone["1,4-Naphthoquinone"]
    CultureMech_002344 --> ing19_1_4_Naphthoquinone
    CHEBI_27418["CHEBI:27418"]
    ing19_1_4_Naphthoquinone -.-> CHEBI_27418
    ing20_Nicotinamide["Nicotinamide"]
    CultureMech_002344 --> ing20_Nicotinamide
    CHEBI_17154["CHEBI:17154"]
    ing20_Nicotinamide -.-> CHEBI_17154
    ing21_Hemin["Hemin"]
    CultureMech_002344 --> ing21_Hemin
    CHEBI_50385["CHEBI:50385"]
    ing21_Hemin -.-> CHEBI_50385
    ing22_NaOH["NaOH"]
    CultureMech_002344 --> ing22_NaOH
    CHEBI_32145["CHEBI:32145"]
    ing22_NaOH -.-> CHEBI_32145
    ing23_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
    CultureMech_002344 --> ing23_Na2SeO3_x_5_H2O
    CHEBI_131361["CHEBI:131361"]
    ing23_Na2SeO3_x_5_H2O -.-> CHEBI_131361
    ing24_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
    CultureMech_002344 --> ing24_Na2WO4_x_2_H2O
    CHEBI_63939["CHEBI:63939"]
    ing24_Na2WO4_x_2_H2O -.-> CHEBI_63939
    ing25_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_002344 --> ing25_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing25_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing26_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_002344 --> ing26_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing26_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing27_H3BO3["H3BO3"]
    CultureMech_002344 --> ing27_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing27_H3BO3 -.-> CHEBI_33118
    ing28_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_002344 --> ing28_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing28_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing29_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_002344 --> ing29_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing29_CuCl2_x_2_H2O -.-> CHEBI_86318
    CultureMech_002344 --> more["...2 more"]

Ingredients (32)

# Ingredient Concentration Ontology Notes
1 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.0329341 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
2 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.0489022 G_PER_L CHEBI:86158
calcium chloride dihydrate
3 K2SO4
2K.O4S 174.259 g/mol
0.116101 G_PER_L CHEBI:32036
potassium sulfate
4 NaNO3
NO3.Na 84.994 g/mol
0.0565536 G_PER_L CHEBI:63005
sodium nitrate
5 KH2PO4
H2O4P.K 136.084 g/mol
1 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
6 CeCl3
CeCl3 246.475 g/mol
0.1 G_PER_L CHEBI:35458
cerium trichloride
7 NaHCO3
CHO3.Na 84.006 g/mol
3 G_PER_L CHEBI:32139
sodium hydrogencarbonate
8 Na2-EDTA
C10H14N2O8.2Na 336.208 g/mol
0.998004 G_PER_L CHEBI:64734
EDTA disodium salt (anhydrous)
9 FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
0.499002 G_PER_L CHEBI:86249
iron dichloride tetrahydrate
10 Biotin
C10H16N2O3S 244.316 g/mol
3.32668e-05 G_PER_L CHEBI:15956
biotin
11 Folic acid
C19H19N7O6 441.404 g/mol
3.32668e-05 G_PER_L CHEBI:27470
folic acid
12 Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
0.000166334 G_PER_L CHEBI:30961
pyridoxine hydrochloride
13 Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
8.3167e-05 G_PER_L CHEBI:49105
thiamine hydrochloride
14 Riboflavin
C17H20N4O6 376.369 g/mol
8.3167e-05 G_PER_L CHEBI:17015
riboflavin
15 Nicotinic acid
C6H5NO2 123.111 g/mol
8.3167e-05 G_PER_L CHEBI:15940
nicotinic acid
16 Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
8.3167e-05 G_PER_L CHEBI:31345
Calcium pantothenate
17 Vitamin B12 1.66334e-05 G_PER_L CHEBI:176843
vitamin B12
18 p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
8.3167e-05 G_PER_L CHEBI:30753
4-aminobenzoic acid
19 Lipoic acid
C8H14O2S2 206.332 g/mol
8.3167e-05 G_PER_L CHEBI:16494
lipoic acid
20 1,4-Naphthoquinone
C10H6O2 158.156 g/mol
6.65336e-05 G_PER_L CHEBI:27418
1,4-naphthoquinone
21 Nicotinamide
C6H6N2O 122.127 g/mol
8.3167e-05 G_PER_L CHEBI:17154
nicotinamide
22 Hemin
C34H32ClFeN4O4 651.952 g/mol
1.66334e-05 G_PER_L CHEBI:50385
hemin
23 NaOH
HO.Na 39.997 g/mol
0.4 G_PER_L CHEBI:32145
sodium hydroxide
24 Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
0.006 G_PER_L CHEBI:131361
disodium selenite pentahydrate
25 Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
0.008 G_PER_L CHEBI:63939
sodium tungstate dihydrate
26 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.1 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
27 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.03 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
28 H3BO3
H3BO3 61.833 g/mol
0.3 G_PER_L CHEBI:33118
boric acid
29 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.2 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
30 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.01 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
31 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.02 G_PER_L CHEBI:34887
nickel dichloride
32 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.03 G_PER_L CHEBI:75213
sodium molybdate dihydrate

Preparation

  1. Mix components thoroughly. For preparation of solid medium, add 15.0 g/L agarose. Autoclave and, after cooling to 60C, add the following solutions (autoclaved or *filter-sterilized):
  2. Adjust pH to 6.5 - 7.0 with 0.2 N H2SO4 solution (autoclaved). Cultivate under an air containing 10% (v/v) methane in gas tight flasks or jars leaving &; 80% gas phase by volume.
  3. Trace element solution:
  4. Vitamins solution:
  5. Adjust pH to 3.6.

Solutions

Trace element solution

Dose: 1 G_PER_L

See mediadive_6187_Trace_element_solution.yaml for composition

Vitamins solution

Dose: 0.1 G_PER_L

See mediadive_5788_Vitamins_solution.yaml for composition

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:18.886013Z mediadive-import Imported from MediaDive Source: JCM, ID: J1171
2026-03-09T08:04:52.697757+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 2 solution(s) from ingredients
2026-03-13T06:19:12.540533Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002344
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name; Added default solution name
2026-03-17T06:30:32.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:25.532330+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "M39 METHYLOMONAS MEDIUM" to "m39_methylomonas_medium" for consistent matching
2026-05-16T09:03:39.504138+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1
2026-06-05T07:41:11.851243+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 31 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:00.701290+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.