caenicola_mobile_medium

CultureMech:002346 bacterial COMPLEX LIQUID
Original name: CAENICOLA MOBILE MEDIUM

Overview

External ontology
mediadive.medium:J1175 (source link unavailable) — CAENICOLA MOBILE MEDIUM
Applications
  • Microbial cultivation
pH
7.5
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1175

Composition

flowchart LR
    CultureMech_002346["caenicola_mobile_medium"]
    ing0_KH2PO4["KH2PO4"]
    CultureMech_002346 --> ing0_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing0_KH2PO4 -.-> CHEBI_63036
    ing1_K2HPO4["K2HPO4"]
    CultureMech_002346 --> ing1_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing1_K2HPO4 -.-> CHEBI_131527
    ing2_NH4Cl["NH4Cl"]
    CultureMech_002346 --> ing2_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing2_NH4Cl -.-> CHEBI_31206
    ing3_NaCl["NaCl"]
    CultureMech_002346 --> ing3_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing3_NaCl -.-> CHEBI_26710
    ing4_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_002346 --> ing4_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing4_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing5_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_002346 --> ing5_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing5_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing6_KCl["KCl"]
    CultureMech_002346 --> ing6_KCl
    CHEBI_32588["CHEBI:32588"]
    ing6_KCl -.-> CHEBI_32588
    ing7_Yeast_extract["Yeast extract"]
    CultureMech_002346 --> ing7_Yeast_extract
    ing8_Tryptone["Tryptone"]
    CultureMech_002346 --> ing8_Tryptone
    ing9_Casamino_acids["Casamino acids"]
    CultureMech_002346 --> ing9_Casamino_acids
    ing10_L_Cysteine["L-Cysteine"]
    CultureMech_002346 --> ing10_L_Cysteine
    CHEBI_17561["CHEBI:17561"]
    ing10_L_Cysteine -.-> CHEBI_17561
    ing11_L_Arginine["L-Arginine"]
    CultureMech_002346 --> ing11_L_Arginine
    CHEBI_16467["CHEBI:16467"]
    ing11_L_Arginine -.-> CHEBI_16467
    ing12_L_Histidine["L-Histidine"]
    CultureMech_002346 --> ing12_L_Histidine
    CHEBI_15971["CHEBI:15971"]
    ing12_L_Histidine -.-> CHEBI_15971
    ing13_Resazurin["Resazurin"]
    CultureMech_002346 --> ing13_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing13_Resazurin -.-> CHEBI_8806
    ing14_NaHCO3["NaHCO3"]
    CultureMech_002346 --> ing14_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing14_NaHCO3 -.-> CHEBI_32139
    ing15_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_002346 --> ing15_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing15_Na2S_x_9_H2O -.-> CHEBI_76209
    ing16_HCl["HCl"]
    CultureMech_002346 --> ing16_HCl
    CHEBI_17883["CHEBI:17883"]
    ing16_HCl -.-> CHEBI_17883
    ing17_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
    CultureMech_002346 --> ing17_FeCl2_x_4_H2O
    CHEBI_86249["CHEBI:86249"]
    ing17_FeCl2_x_4_H2O -.-> CHEBI_86249
    ing18_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_002346 --> ing18_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing18_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing19_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_002346 --> ing19_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing19_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing20_ZnCl2["ZnCl2"]
    CultureMech_002346 --> ing20_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing20_ZnCl2 -.-> CHEBI_49976
    ing21_H3BO3["H3BO3"]
    CultureMech_002346 --> ing21_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing21_H3BO3 -.-> CHEBI_33118
    ing22_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_002346 --> ing22_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing22_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing23_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_002346 --> ing23_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing23_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing24_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_002346 --> ing24_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing24_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing25_AlCl3["AlCl3"]
    CultureMech_002346 --> ing25_AlCl3
    CHEBI_30114["CHEBI:30114"]
    ing25_AlCl3 -.-> CHEBI_30114
    ing26_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
    CultureMech_002346 --> ing26_Na2WO4_x_2_H2O
    CHEBI_63939["CHEBI:63939"]
    ing26_Na2WO4_x_2_H2O -.-> CHEBI_63939
    ing27_Biotin["Biotin"]
    CultureMech_002346 --> ing27_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing27_Biotin -.-> CHEBI_15956
    ing28_Folic_acid["Folic acid"]
    CultureMech_002346 --> ing28_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing28_Folic_acid -.-> CHEBI_27470
    ing29_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
    CultureMech_002346 --> ing29_Pyridoxine_hydrochloride
    CHEBI_30961["CHEBI:30961"]
    ing29_Pyridoxine_hydrochloride -.-> CHEBI_30961
    CultureMech_002346 --> more["...7 more"]

Ingredients (37)

# Ingredient Concentration Ontology Notes
1 KH2PO4
H2O4P.K 136.084 g/mol
0.49505 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
2 K2HPO4
HO4P.2K 174.174 g/mol
0.49505 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
3 NH4Cl
Cl.H4N 53.492 g/mol
0.49505 G_PER_L CHEBI:31206
ammonium chloride
4 NaCl
Cl.Na 58.443 g/mol
0.39604 G_PER_L CHEBI:26710
sodium chloride
5 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
0.29703 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
6 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.049505 G_PER_L CHEBI:86158
calcium chloride dihydrate
7 KCl
Cl.K 74.551 g/mol
0.39604 G_PER_L CHEBI:32588
potassium chloride
8 Yeast extract 1.9802 G_PER_L
9 Tryptone 1.9802 G_PER_L
10 Casamino acids 1.9802 G_PER_L
11 L-Cysteine
C3H7NO2S 121.161 g/mol
2.37624 G_PER_L CHEBI:17561
L-cysteine
12 L-Arginine
C6H14N4O2 174.204 g/mol
4.15842 G_PER_L CHEBI:16467
L-arginine
13 L-Histidine
C6H9N3O2 155.157 g/mol
4.15842 G_PER_L CHEBI:15971
L-histidine
14 Resazurin
C12H7NO4 229.191 g/mol
0.000990099 G_PER_L CHEBI:8806
Resazurin
15 NaHCO3
CHO3.Na 84.006 g/mol
25 G_PER_L CHEBI:32139
sodium hydrogencarbonate
16 Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
8 G_PER_L CHEBI:76209
sodium sulfide nonahydrate
17 HCl
HCl 36.461 g/mol
3.16832 G_PER_L CHEBI:17883
hydrogen chloride
18 FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
1.9802 G_PER_L CHEBI:86249
iron dichloride tetrahydrate
19 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.247525 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
20 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.0990099 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
21 ZnCl2
2Cl.Zn 136.296 g/mol
0.0693069 G_PER_L CHEBI:49976
zinc dichloride
22 H3BO3
H3BO3 61.833 g/mol
0.00594059 G_PER_L CHEBI:33118
boric acid
23 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.039604 G_PER_L CHEBI:75213
sodium molybdate dihydrate
24 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.0693069 G_PER_L CHEBI:34887
nickel dichloride
25 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.0019802 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
26 AlCl3
AlCl3 133.341 g/mol
0.0247525 G_PER_L CHEBI:30114
aluminium trichloride
27 Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
0.00594059 G_PER_L CHEBI:63939
sodium tungstate dihydrate
28 Biotin
C10H16N2O3S 244.316 g/mol
0.002 G_PER_L CHEBI:15956
biotin
29 Folic acid
C19H19N7O6 441.404 g/mol
0.002 G_PER_L CHEBI:27470
folic acid
30 Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
0.01 G_PER_L CHEBI:30961
pyridoxine hydrochloride
31 Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
0.005 G_PER_L CHEBI:49105
thiamine hydrochloride
32 Riboflavin
C17H20N4O6 376.369 g/mol
0.005 G_PER_L CHEBI:17015
riboflavin
33 Nicotinic acid
C6H5NO2 123.111 g/mol
0.005 G_PER_L CHEBI:15940
nicotinic acid
34 Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.005 G_PER_L CHEBI:31345
Calcium pantothenate
35 Vitamin B12 0.0001 G_PER_L CHEBI:176843
vitamin B12
36 p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
0.005 G_PER_L CHEBI:30753
4-aminobenzoic acid
37 Lipoic acid
C8H14O2S2 206.332 g/mol
0.005 G_PER_L CHEBI:16494
lipoic acid

Preparation

  1. Mix components thoroughly, adjust pH to 7.5 with KOH and autoclave under a N2-CO2 (4:1, v/v) gas mixture. After cooling, add the following solutions (filter-sterilized):
  2. Aseptically and anaerobically distribute the medium into culture vessels (e.g., 20 ml medium in 60 ml serum bottles) under a N2-CO2 (4:1, v/v) gas mixture and seal with butyl rubber stoppers. Then, add per liter the following solution (autoclaved and stored under N2):
  3. Readjust pH to 7.5, if necessary.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:18.902214Z mediadive-import Imported from MediaDive Source: JCM, ID: J1175
2026-01-28T19:48:12.434352Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:19:12.570642Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002346
2026-03-17T06:29:51.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:25.566835+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "CAENICOLA MOBILE MEDIUM" to "caenicola_mobile_medium" for consistent matching
2026-06-05T07:40:51.962952+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 35 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-08T23:19:36.088946+00:00 chebi-regrounding-v1.0 Corrected CHEBI ingredient grounding (shared-id regrounding audit, G21/G22/G24) Part of the shared-id CHEBI regrounding audit (G21/G22/G24): one or more ingredient groundings on this record that had been collapsed onto a chemically non-specific or shared CHEBI id were re-grounded to the correct chemically-specific term, verified against OAK chebi.db rdfs:label. See reports/chebi_grounding_audit.md.
2026-06-10T07:46:54.078785+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.