methanothermococcus_hhb_medium

CultureMech:002361 archaea DEFINED LIQUID
Original name: METHANOTHERMOCOCCUS HHB MEDIUM

Overview

External ontology
mediadive.medium:J1194 (source link unavailable) — METHANOTHERMOCOCCUS HHB MEDIUM
Applications
  • Microbial cultivation
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1194

Composition

flowchart LR
    CultureMech_002361["methanothermococcus_hhb_medium"]
    ing0_NH4Cl["NH4Cl"]
    CultureMech_002361 --> ing0_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing0_NH4Cl -.-> CHEBI_31206
    ing1_K2HPO4["K2HPO4"]
    CultureMech_002361 --> ing1_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing1_K2HPO4 -.-> CHEBI_131527
    ing2_KCl["KCl"]
    CultureMech_002361 --> ing2_KCl
    CHEBI_32588["CHEBI:32588"]
    ing2_KCl -.-> CHEBI_32588
    ing3_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_002361 --> ing3_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing3_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing4_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_002361 --> ing4_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing4_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing5_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_002361 --> ing5_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing5_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing6_NaCl["NaCl"]
    CultureMech_002361 --> ing6_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing6_NaCl -.-> CHEBI_26710
    ing7_Fe_NH4_2_SO4_2_x_6_H2O["Fe(NH4)2(SO4)2 x 6 H2O"]
    CultureMech_002361 --> ing7_Fe_NH4_2_SO4_2_x_6_H2O
    CHEBI_76181["CHEBI:76181"]
    ing7_Fe_NH4_2_SO4_2_x_6_H2O -.-> CHEBI_76181
    ing8_Resazurin["Resazurin"]
    CultureMech_002361 --> ing8_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing8_Resazurin -.-> CHEBI_8806
    ing9_NaHCO3["NaHCO3"]
    CultureMech_002361 --> ing9_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing9_NaHCO3 -.-> CHEBI_32139
    ing10_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_002361 --> ing10_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing10_Na2S_x_9_H2O -.-> CHEBI_76209
    ing11_Biotin["Biotin"]
    CultureMech_002361 --> ing11_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing11_Biotin -.-> CHEBI_15956
    ing12_Folic_acid["Folic acid"]
    CultureMech_002361 --> ing12_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing12_Folic_acid -.-> CHEBI_27470
    ing13_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
    CultureMech_002361 --> ing13_Pyridoxine_hydrochloride
    CHEBI_30961["CHEBI:30961"]
    ing13_Pyridoxine_hydrochloride -.-> CHEBI_30961
    ing14_Thiamine_HCl["Thiamine HCl"]
    CultureMech_002361 --> ing14_Thiamine_HCl
    CHEBI_49105["CHEBI:49105"]
    ing14_Thiamine_HCl -.-> CHEBI_49105
    ing15_Riboflavin["Riboflavin"]
    CultureMech_002361 --> ing15_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing15_Riboflavin -.-> CHEBI_17015
    ing16_Nicotinic_acid["Nicotinic acid"]
    CultureMech_002361 --> ing16_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing16_Nicotinic_acid -.-> CHEBI_15940
    ing17_Calcium_pantothenate["Calcium pantothenate"]
    CultureMech_002361 --> ing17_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing17_Calcium_pantothenate -.-> CHEBI_31345
    ing18_Vitamin_B12["Vitamin B12"]
    CultureMech_002361 --> ing18_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing18_Vitamin_B12 -.-> CHEBI_176843
    ing19_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_002361 --> ing19_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing19_p_Aminobenzoic_acid -.-> CHEBI_30753
    ing20_Lipoic_acid["Lipoic acid"]
    CultureMech_002361 --> ing20_Lipoic_acid
    CHEBI_16494["CHEBI:16494"]
    ing20_Lipoic_acid -.-> CHEBI_16494
    ing21_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
    CultureMech_002361 --> ing21_FeCl2_x_4_H2O
    CHEBI_86249["CHEBI:86249"]
    ing21_FeCl2_x_4_H2O -.-> CHEBI_86249
    ing22_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_002361 --> ing22_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing22_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing23_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_002361 --> ing23_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing23_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing24_ZnCl2["ZnCl2"]
    CultureMech_002361 --> ing24_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing24_ZnCl2 -.-> CHEBI_49976
    ing25_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_002361 --> ing25_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing25_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing26_H3BO3["H3BO3"]
    CultureMech_002361 --> ing26_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing26_H3BO3 -.-> CHEBI_33118
    ing27_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_002361 --> ing27_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing27_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing28_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_002361 --> ing28_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing28_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing29_AlCl3["AlCl3"]
    CultureMech_002361 --> ing29_AlCl3
    CHEBI_30114["CHEBI:30114"]
    ing29_AlCl3 -.-> CHEBI_30114

Ingredients (30)

# Ingredient Concentration Ontology Notes
1 NH4Cl
Cl.H4N 53.492 g/mol
0.479386 G_PER_L CHEBI:31206
ammonium chloride
2 K2HPO4
HO4P.2K 174.174 g/mol
0.134228 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
3 KCl
Cl.K 74.551 g/mol
0.316395 G_PER_L CHEBI:32588
potassium chloride
4 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
4.00767 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
5 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
3.25983 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
6 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.134228 G_PER_L CHEBI:86158
calcium chloride dihydrate
7 NaCl
Cl.Na 58.443 g/mol
19.1755 G_PER_L CHEBI:26710
sodium chloride
8 Fe(NH4)2(SO4)2 x 6 H2O
Fe.6H2O.2H4N.2O4S 392.139 g/mol
0.00191755 G_PER_L CHEBI:76181
ferrous ammonium sulfate hexahydrate
9 Resazurin
C12H7NO4 229.191 g/mol
0.000958773 G_PER_L CHEBI:8806
Resazurin
10 NaHCO3
CHO3.Na 84.006 g/mol
12 G_PER_L CHEBI:32139
sodium hydrogencarbonate
11 Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
10 G_PER_L CHEBI:76209
sodium sulfide nonahydrate
12 Biotin
C10H16N2O3S 244.316 g/mol
0.002 G_PER_L CHEBI:15956
biotin
13 Folic acid
C19H19N7O6 441.404 g/mol
0.002 G_PER_L CHEBI:27470
folic acid
14 Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
0.01 G_PER_L CHEBI:30961
pyridoxine hydrochloride
15 Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
0.005 G_PER_L CHEBI:49105
thiamine hydrochloride
16 Riboflavin
C17H20N4O6 376.369 g/mol
0.005 G_PER_L CHEBI:17015
riboflavin
17 Nicotinic acid
C6H5NO2 123.111 g/mol
0.005 G_PER_L CHEBI:15940
nicotinic acid
18 Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.005 G_PER_L CHEBI:31345
Calcium pantothenate
19 Vitamin B12 0.0001 G_PER_L CHEBI:176843
vitamin B12
20 p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
0.005 G_PER_L CHEBI:30753
4-aminobenzoic acid
21 Lipoic acid
C8H14O2S2 206.332 g/mol
0.005 G_PER_L CHEBI:16494
lipoic acid
22 FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
1.99 G_PER_L CHEBI:86249
iron dichloride tetrahydrate
23 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.198 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
24 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.13 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
25 ZnCl2
2Cl.Zn 136.296 g/mol
0.136 G_PER_L CHEBI:49976
zinc dichloride
26 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.0017 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
27 H3BO3
H3BO3 61.833 g/mol
0.0062 G_PER_L CHEBI:33118
boric acid
28 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.024 G_PER_L CHEBI:75213
sodium molybdate dihydrate
29 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.013 G_PER_L CHEBI:34887
nickel dichloride
30 AlCl3
AlCl3 133.341 g/mol
0.013 G_PER_L CHEBI:30114
aluminium trichloride

Preparation

  1. Mix components thoroughly. Bring to a boil and cool down under a H2-CO2 (4:1, v/v) gas stream. Distribute the medium into culture vessels (e.g., 20 ml medium in 120 ml serum bottles) under the same gas mixture, seal with butyl rubber stoppers and autoclave. After cooling, aseptically and anaerobically add per liter the following solutions (autoclaved or *filter-sterilized):
  2. Pressurize the inoculated bottles to 200 kPa H2-CO2 (80:20, v/v).

Solutions

Selenite-tungstate solution

Dose: 1 G_PER_L

See mediadive_5543_Selenite-tungstate_solution.yaml for composition

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:18.970295Z mediadive-import Imported from MediaDive Source: JCM, ID: J1194
2026-01-28T19:48:07.339214Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-09T08:04:11.839215+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 1 solution(s) from ingredients
2026-03-13T06:19:12.709254Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002361
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name
2026-03-17T06:29:40.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:25.733229+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "METHANOTHERMOCOCCUS HHB MEDIUM" to "methanothermococcus_hhb_medium" for consistent matching
2026-05-16T09:04:22.862226+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1
2026-06-05T07:41:32.863820+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 30 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:07.464956+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-09-10T00:00:00-07:00 repair_archaeoglobus_mcr_score20.py LINKED_ARCHAEOGLOBUS_MCR_SCORE20_CHILDREN Added or refreshed reciprocal supplemented-variant link for archaea/archaeoglobus_mcr_medium.yaml.