mineral_medium_brunner_supplemented_with_toluene

CultureMech:002366 bacterial DEFINED SOLID_AGAR
Original name: MINERAL MEDIUM (BRUNNER) SUPPLEMENTED WITH TOLUENE

Overview

External ontology
mediadive.medium:J1199 (source link unavailable) — MINERAL MEDIUM (BRUNNER) SUPPLEMENTED WITH TOLUENE
Applications
  • Microbial cultivation
pH
6.9
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1199

Composition

flowchart LR
    CultureMech_002366["mineral_medium_brunner_supplemented_with_toluene"]
    ing0__NH4_2SO4["(NH4)2SO4"]
    CultureMech_002366 --> ing0__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing0__NH4_2SO4 -.-> CHEBI_62946
    ing1_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_002366 --> ing1_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing2_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_002366 --> ing2_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing2_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing3_Agar["Agar"]
    CultureMech_002366 --> ing3_Agar
    CHEBI_2509["CHEBI:2509"]
    ing3_Agar -.-> CHEBI_2509
    ing4_Na2HPO4_x_2_H2O["Na2HPO4 x 2 H2O"]
    CultureMech_002366 --> ing4_Na2HPO4_x_2_H2O
    CHEBI_91258["CHEBI:91258"]
    ing4_Na2HPO4_x_2_H2O -.-> CHEBI_91258
    ing5_KH2PO4["KH2PO4"]
    CultureMech_002366 --> ing5_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing5_KH2PO4 -.-> CHEBI_63036
    ing6_EDTA["EDTA"]
    CultureMech_002366 --> ing6_EDTA
    CHEBI_4735["CHEBI:4735"]
    ing6_EDTA -.-> CHEBI_4735
    ing7_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_002366 --> ing7_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing7_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing8_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_002366 --> ing8_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing8_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing9_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_002366 --> ing9_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing9_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing10_H3BO3["H3BO3"]
    CultureMech_002366 --> ing10_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing10_H3BO3 -.-> CHEBI_33118
    ing11_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_002366 --> ing11_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing11_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing12_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_002366 --> ing12_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing12_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing13_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_002366 --> ing13_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing13_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing14_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_002366 --> ing14_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing14_Na2MoO4_x_2_H2O -.-> CHEBI_75213

Ingredients (15)

# Ingredient Concentration Ontology Notes
1 (NH4)2SO4
2H4N.O4S 132.141 g/mol
0.248756 G_PER_L CHEBI:62946
ammonium sulfate
2 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.0995025 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
3 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.0248756 G_PER_L CHEBI:86158
calcium chloride dihydrate
4 Agar 7.46269 G_PER_L CHEBI:2509
agar
5 Na2HPO4 x 2 H2O
2H2O.HO4P.2Na 177.988 g/mol
1.21393 G_PER_L CHEBI:91258
disodium hydrogenphosphate dihydrate
6 KH2PO4
H2O4P.K 136.084 g/mol
0.756219 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
7 EDTA
C10H16N2O8 292.244 g/mol
0.5 G_PER_L CHEBI:4735
ethylenediaminetetraacetic acid
8 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.2 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
9 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.1 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
 [Collapsed 2 identical duplicates: 0.1, 0.1]
10 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.03 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
 [Collapsed 2 identical duplicates: 0.03, 0.03]
11 H3BO3
H3BO3 61.833 g/mol
0.3 G_PER_L CHEBI:33118
boric acid
 [Collapsed 2 identical duplicates: 0.3, 0.3]
12 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.2 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
 [Collapsed 2 identical duplicates: 0.2, 0.2]
13 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.01 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
 [Collapsed 2 identical duplicates: 0.01, 0.01]
14 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.02 G_PER_L CHEBI:34887
nickel dichloride
 [Collapsed 2 identical duplicates: 0.02, 0.02]
15 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.03 G_PER_L CHEBI:75213
sodium molybdate dihydrate
 [Collapsed 2 identical duplicates: 0.03, 0.03]

Preparation

  1. Adjust pH to 6.9. After autoclaving, aseptically add the phosphates solution to the medium. After inoculation, put several drops of toluene inside of lid of petri dishes.
  2. Phosphates solution:
  3. Adjust pH to 6.9.
  4. Adjust pH to 3.6.

Solutions

Phosphates solution

Dose: 100 G_PER_L

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:18.985165Z mediadive-import Imported from MediaDive Source: JCM, ID: J1199
2026-03-09T08:04:17.545927+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 1 solution(s) from ingredients
2026-03-13T03:08:45.123041Z data-quality-cleanup-v1.0 Data quality fixes Merged 7 duplicate ingredient(s)
2026-03-13T06:19:12.746469Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002366
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name
2026-03-17T06:29:47.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:25.775933+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "MINERAL MEDIUM (BRUNNER) SUPPLEMENTED WITH TOLUENE" to "mineral_medium_brunner_supplemented_with_toluene" for consistent matching
2026-05-16T09:04:23.659556+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1
2026-06-05T07:41:34.098920+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 15 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:07.862783+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-09-02T07:38:49.770224+00:00 repair_merged_duplicates.py REPAIRED_SUMMED_DUPLICATE_MERGE Collapsed 7 ingredient row(s) whose concentration was the SUM of identical duplicate values written by cleanup_media_quality.py. Every merged part was the same value, so the pre-merge value is unambiguous and no source lookup was needed. Rows whose merged parts differ were left untouched.