Overview
- External ontology
- mediadive.medium:J11 (source link unavailable) — LACTOBACILLUS MEDIUM I
- Applications
-
- Microbial cultivation
- pH
- 6.0
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=11
Composition
flowchart LR
CultureMech_002368["lactobacillus_medium_i"]
ing0_Tryptone["Tryptone"]
CultureMech_002368 --> ing0_Tryptone
ing1_Tryptose["Tryptose"]
CultureMech_002368 --> ing1_Tryptose
ing2_Yeast_extract["Yeast extract"]
CultureMech_002368 --> ing2_Yeast_extract
ing3_Tomato_juice["Tomato juice"]
CultureMech_002368 --> ing3_Tomato_juice
ing4_Liver_extract_concentrate["Liver extract concentrate"]
CultureMech_002368 --> ing4_Liver_extract_concentrate
ing5_Tween_80["Tween 80"]
CultureMech_002368 --> ing5_Tween_80
CHEBI_53426["CHEBI:53426"]
ing5_Tween_80 -.-> CHEBI_53426
ing6_Glucose["Glucose"]
CultureMech_002368 --> ing6_Glucose
CHEBI_17234["CHEBI:17234"]
ing6_Glucose -.-> CHEBI_17234
ing7_Lactose["Lactose"]
CultureMech_002368 --> ing7_Lactose
CHEBI_17716["CHEBI:17716"]
ing7_Lactose -.-> CHEBI_17716
ing8_Agar["Agar"]
CultureMech_002368 --> ing8_Agar
CHEBI_2509["CHEBI:2509"]
ing8_Agar -.-> CHEBI_2509
Ingredients (9)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Tryptone | 16.6667 G_PER_L |
MICRO:0000182 (source link unavailable)
Tryptone
|
|
| 2 | Tryptose | 4.16667 G_PER_L |
MICRO:0000183 (source link unavailable)
Tryptose
|
|
| 3 | Yeast extract | 4.16667 G_PER_L |
FOODON:03315426
Yeast extract
|
|
| 4 | Tomato juice | 200 G_PER_L |
FOODON:03301454
Tomato juice
|
( filtered, pH 7.0) |
| 5 | Liver extract concentrate | 0.833333 G_PER_L |
MICRO:0001363 (source link unavailable)
Liver extract concentrate
|
|
| 6 |
Tween 80
(C2H4O)w.(C2H4O)x.(C2H4O)y.(C2H4O)z.C24H44O6 604.822 g/mol
|
0.0416667 G_PER_L |
CHEBI:53426
polysorbate 80
|
|
| 7 |
Glucose
C6H12O6 180.156 g/mol
|
2.5 G_PER_L |
CHEBI:17234
glucose
|
|
| 8 |
Lactose
C12H22O11 342.297 g/mol
|
1.66667 G_PER_L |
CHEBI:17716
lactose
|
|
| 9 | Agar | 12.5 G_PER_L |
CHEBI:2509
agar
|
Preparation
- Adjust pH to 6.0.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:15.576080Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J11 |
| 2026-03-13T06:19:12.754947Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002368 |
| 2026-03-17T06:30:23.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:25.786369+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "LACTOBACILLUS MEDIUM I" to "lactobacillus_medium_i" for consistent matching |
| 2026-06-05T07:41:10.705560+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 4 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-09-13T00:00:00-07:00 | repair_bacterial_score10_exact_terms_batch3.py | GROUNDED_BACTERIAL_SCORE10_EXACT_TERMS_BATCH3 | Applied local mappings for Liver extract concentrate, Tomato juice, Tryptone, Tryptose, Yeast extract. |
| 2026-10-07T14:33:27.670564+00:00 | codex | CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE | Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged. |