desulfovibrio_fss_1_medium

CultureMech:002374 bacterial COMPLEX LIQUID
Original name: DESULFOVIBRIO FSS-1 MEDIUM

Overview

External ontology
mediadive.medium:J1205 (source link unavailable) — DESULFOVIBRIO FSS-1 MEDIUM
Applications
  • Microbial cultivation
pH
7.0
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1205

Composition

flowchart LR
    CultureMech_002374["desulfovibrio_fss_1_medium"]
    ing0_NH4Cl["NH4Cl"]
    CultureMech_002374 --> ing0_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing0_NH4Cl -.-> CHEBI_31206
    ing1_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_002374 --> ing1_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing2_Yeast_extract["Yeast extract"]
    CultureMech_002374 --> ing2_Yeast_extract
    ing3_Resazurin["Resazurin"]
    CultureMech_002374 --> ing3_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing3_Resazurin -.-> CHEBI_8806
    ing4_Potassium_phosphate_buffer["Potassium phosphate buffer"]
    CultureMech_002374 --> ing4_Potassium_phosphate_buffer
    ing5_Sodium_lactate["Sodium lactate"]
    CultureMech_002374 --> ing5_Sodium_lactate
    CHEBI_75228["CHEBI:75228"]
    ing5_Sodium_lactate -.-> CHEBI_75228
    ing6_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
    CultureMech_002374 --> ing6_L_Cysteine_HCl_x_H2O
    CHEBI_91248["CHEBI:91248"]
    ing6_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
    ing7_Fe_III__EDTA["Fe(III)-EDTA"]
    CultureMech_002374 --> ing7_Fe_III__EDTA
    CHEBI_30729["CHEBI:30729"]
    ing7_Fe_III__EDTA -.-> CHEBI_30729
    ing8_Thiamine_HCl["Thiamine HCl"]
    CultureMech_002374 --> ing8_Thiamine_HCl
    CHEBI_49105["CHEBI:49105"]
    ing8_Thiamine_HCl -.-> CHEBI_49105
    ing9_Myo_inositol["Myo-inositol"]
    CultureMech_002374 --> ing9_Myo_inositol
    CHEBI_17268["CHEBI:17268"]
    ing9_Myo_inositol -.-> CHEBI_17268
    ing10_Calcium_pantothenate["Calcium pantothenate"]
    CultureMech_002374 --> ing10_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing10_Calcium_pantothenate -.-> CHEBI_31345
    ing11_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_002374 --> ing11_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing11_p_Aminobenzoic_acid -.-> CHEBI_30753
    ing12_Vitamin_B12["Vitamin B12"]
    CultureMech_002374 --> ing12_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing12_Vitamin_B12 -.-> CHEBI_176843
    ing13_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
    CultureMech_002374 --> ing13_Pyridoxine_hydrochloride
    CHEBI_30961["CHEBI:30961"]
    ing13_Pyridoxine_hydrochloride -.-> CHEBI_30961
    ing14_Nicotinic_acid["Nicotinic acid"]
    CultureMech_002374 --> ing14_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing14_Nicotinic_acid -.-> CHEBI_15940
    ing15_Biotin["Biotin"]
    CultureMech_002374 --> ing15_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing15_Biotin -.-> CHEBI_15956
    ing16_Folic_acid["Folic acid"]
    CultureMech_002374 --> ing16_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing16_Folic_acid -.-> CHEBI_27470

Ingredients (17)

# Ingredient Concentration Ontology Notes
1 NH4Cl
Cl.H4N 53.492 g/mol
0.298211 G_PER_L CHEBI:31206
ammonium chloride
2 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.0994036 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
3 Yeast extract 0.0198807 G_PER_L
4 Resazurin
C12H7NO4 229.191 g/mol
0.000497018 G_PER_L CHEBI:8806
Resazurin
5 Potassium phosphate buffer 5.6 G_PER_L
6 Sodium lactate
C3H5O3.Na 112.06 g/mol
20 G_PER_L CHEBI:75228
sodium lactate
7 L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
8 G_PER_L CHEBI:91248
L-cysteine hydrochloride hydrate
8 Fe(III)-EDTA
C10H12N2O8.Fe 344.057 g/mol
5 G_PER_L CHEBI:30729
ethylenediaminetetraacetatoferrate(1-)
9 Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
0.9 G_PER_L CHEBI:49105
thiamine hydrochloride
10 Myo-inositol
C6H12O6 180.156 g/mol
0.4 G_PER_L CHEBI:17268
myo-inositol
11 Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.04 G_PER_L CHEBI:31345
Calcium pantothenate
12 p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
0.05 G_PER_L CHEBI:30753
4-aminobenzoic acid
13 Vitamin B12 0.05 G_PER_L CHEBI:176843
vitamin B12
14 Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
0.04 G_PER_L CHEBI:30961
pyridoxine hydrochloride
15 Nicotinic acid
C6H5NO2 123.111 g/mol
0.04 G_PER_L CHEBI:15940
nicotinic acid
16 Biotin
C10H16N2O3S 244.316 g/mol
0.001 G_PER_L CHEBI:15956
biotin
17 Folic acid
C19H19N7O6 441.404 g/mol
0.02 G_PER_L CHEBI:27470
folic acid

Preparation

  1. Mix components thoroughly, adjust pH to 7.0 and autoclave under a N2 gas atmosphere. After cooling, aseptically and anaerobically add the following solutions from anaerobic stocks (autoclaved or *filter-sterilized):
  2. Distribute the medium into culture vessels (e.g., 20 ml in Balch tubes) and seal with butyl rubber stoppers.
  3. Use trace minerals (see Medium No. 151) with final 0.4 g/L Na2MoO4 x 2H2O, 0.02 g/L CuSO4 x 5H2O and 0.01 g/L NiCl2 x 6H2O.
  4. Comment: The medium tubes can be stocked under a N2 gas atmosphere. In this case, prior to use, fill the head space with air and allow to stand until the O2 gradient is formed.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:19.006878Z mediadive-import Imported from MediaDive Source: JCM, ID: J1205
2026-03-13T06:19:12.804512Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002374
2026-03-17T06:30:22.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:25.842692+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "DESULFOVIBRIO FSS-1 MEDIUM" to "desulfovibrio_fss_1_medium" for consistent matching
2026-06-05T07:40:57.756763+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 15 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:56.018277+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.