Overview
- External ontology
- mediadive.medium:J1212 (source link unavailable) — MODIFIED MAKKAR'S MEDIUM C
- Applications
-
- Microbial cultivation
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1212
Composition
flowchart LR
CultureMech_002381["modified_makkars_medium_c"]
ing0_Rumen_fluid["Rumen fluid"]
CultureMech_002381 --> ing0_Rumen_fluid
ing1_Yeast_extract["Yeast extract"]
CultureMech_002381 --> ing1_Yeast_extract
ing2_Casitone["Casitone"]
CultureMech_002381 --> ing2_Casitone
ing3_NaHCO3["NaHCO3"]
CultureMech_002381 --> ing3_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing3_NaHCO3 -.-> CHEBI_32139
ing4_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
CultureMech_002381 --> ing4_L_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing4_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
ing5_Resazurin["Resazurin"]
CultureMech_002381 --> ing5_Resazurin
CHEBI_8806["CHEBI:8806"]
ing5_Resazurin -.-> CHEBI_8806
ing6_Cellobiose["Cellobiose"]
CultureMech_002381 --> ing6_Cellobiose
CHEBI_17057["CHEBI:17057"]
ing6_Cellobiose -.-> CHEBI_17057
ing7_K2HPO4["K2HPO4"]
CultureMech_002381 --> ing7_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing7_K2HPO4 -.-> CHEBI_131527
ing8_KH2PO4["KH2PO4"]
CultureMech_002381 --> ing8_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing8_KH2PO4 -.-> CHEBI_63036
ing9__NH4_2SO4["(NH4)2SO4"]
CultureMech_002381 --> ing9__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing9__NH4_2SO4 -.-> CHEBI_62946
ing10_NaCl["NaCl"]
CultureMech_002381 --> ing10_NaCl
CHEBI_26710["CHEBI:26710"]
ing10_NaCl -.-> CHEBI_26710
ing11_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_002381 --> ing11_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing11_MgSO4_x_7_H2O -.-> CHEBI_31795
ing12_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_002381 --> ing12_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing12_CaCl2_x_2_H2O -.-> CHEBI_86158
Ingredients (13)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Rumen fluid | 50 G_PER_L | ( clarified) | |
| 2 | Yeast extract | 2.45098 G_PER_L | ||
| 3 | Casitone | 9.80392 G_PER_L | ||
| 4 |
NaHCO3
CHO3.Na 84.006 g/mol
|
5.88235 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 5 |
L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.980392 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
|
| 6 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.000980392 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 7 |
Cellobiose
C12H22O11 342.297 g/mol
|
20 G_PER_L |
CHEBI:17057
cellobiose
|
|
| 8 |
K2HPO4
HO4P.2K 174.174 g/mol
|
3 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 9 |
KH2PO4
H2O4P.K 136.084 g/mol
|
3 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 10 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
6 G_PER_L |
CHEBI:62946
ammonium sulfate
|
|
| 11 |
NaCl
Cl.Na 58.443 g/mol
|
6 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 12 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.6 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 13 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.6 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Preparation
- Mix components, except L-cysteine x HCl x H2O, and heat the medium to 60C with stirring. Bubble the medium with O2-free CO2 vigorously, then add L-cysteine x HCl x H2O to reduce the medium. Dispense the medium into culture vessels (e.g., 10 ml in Balch type tubes) under a stream of CO2, seal with butyl rubber stoppers and autoclave. Aseptically and anaerobically add per liter the following solution (autoclaved and stored under a N2 atmosphere):
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:19.039543Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J1212 |
| 2026-03-13T06:19:12.874288Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002381 |
| 2026-03-17T06:29:46.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:25.919529+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MODIFIED MAKKAR'S MEDIUM C" to "modified_makkars_medium_c" for consistent matching |
| 2026-06-05T07:41:37.018683+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 10 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:08.700593+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |