Overview
- External ontology
- mediadive.medium:J1213 (source link unavailable) — PYG MEDIUM (J), MODIFIED
- Applications
-
- Microbial cultivation
- pH
- 7.2
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1213
Composition
flowchart LR
CultureMech_002382["pyg_medium_j_modified"]
ing0_Trypticase_peptone["Trypticase peptone"]
CultureMech_002382 --> ing0_Trypticase_peptone
ing1_Peptone["Peptone"]
CultureMech_002382 --> ing1_Peptone
ing2_Yeast_extract["Yeast extract"]
CultureMech_002382 --> ing2_Yeast_extract
ing3_Beef_extract["Beef extract"]
CultureMech_002382 --> ing3_Beef_extract
ing4_Glucose["Glucose"]
CultureMech_002382 --> ing4_Glucose
CHEBI_17234["CHEBI:17234"]
ing4_Glucose -.-> CHEBI_17234
ing5_KH2PO4["KH2PO4"]
CultureMech_002382 --> ing5_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing5_KH2PO4 -.-> CHEBI_63036
ing6_Tween_80["Tween 80"]
CultureMech_002382 --> ing6_Tween_80
CHEBI_53426["CHEBI:53426"]
ing6_Tween_80 -.-> CHEBI_53426
ing7_Resazurin["Resazurin"]
CultureMech_002382 --> ing7_Resazurin
CHEBI_8806["CHEBI:8806"]
ing7_Resazurin -.-> CHEBI_8806
ing8_Rumen_fluid["Rumen fluid"]
CultureMech_002382 --> ing8_Rumen_fluid
ing9_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
CultureMech_002382 --> ing9_L_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing9_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
ing10_Vitamin_K1["Vitamin K1"]
CultureMech_002382 --> ing10_Vitamin_K1
CHEBI_18067["CHEBI:18067"]
ing10_Vitamin_K1 -.-> CHEBI_18067
ing11_Ethanol["Ethanol"]
CultureMech_002382 --> ing11_Ethanol
CHEBI_16236["CHEBI:16236"]
ing11_Ethanol -.-> CHEBI_16236
ing12_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_002382 --> ing12_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing12_CaCl2_x_2_H2O -.-> CHEBI_86158
ing13_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_002382 --> ing13_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing13_MgSO4_x_7_H2O -.-> CHEBI_31795
ing14_K2HPO4["K2HPO4"]
CultureMech_002382 --> ing14_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing14_K2HPO4 -.-> CHEBI_131527
ing15_NaHCO3["NaHCO3"]
CultureMech_002382 --> ing15_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing15_NaHCO3 -.-> CHEBI_32139
ing16_NaCl["NaCl"]
CultureMech_002382 --> ing16_NaCl
CHEBI_26710["CHEBI:26710"]
ing16_NaCl -.-> CHEBI_26710
Ingredients (17)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Trypticase peptone | 4.99501 G_PER_L | ||
| 2 | Peptone | 4.99501 G_PER_L | ||
| 3 | Yeast extract | 9.99001 G_PER_L | ||
| 4 | Beef extract | 4.99501 G_PER_L | ||
| 5 |
Glucose
C6H12O6 180.156 g/mol
|
4.99501 G_PER_L |
CHEBI:17234
glucose
|
|
| 6 |
KH2PO4
H2O4P.K 136.084 g/mol
|
2.998 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
[Merged 2 duplicates: 1.998, 1.0] |
| 7 |
Tween 80
(C2H4O)w.(C2H4O)x.(C2H4O)y.(C2H4O)z.C24H44O6 604.822 g/mol
|
1 G_PER_L |
CHEBI:53426
polysorbate 80
|
|
| 8 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.000999001 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 9 | Rumen fluid | 50 G_PER_L | ( clarified) | |
| 10 |
L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
10 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
|
| 11 |
Vitamin K1
C31H46O2 450.707 g/mol
|
0.1 G_PER_L |
CHEBI:18067
phylloquinone
|
|
| 12 |
Ethanol
C2H6O 46.069 g/mol
|
950 G_PER_L |
CHEBI:16236
ethanol
|
|
| 13 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.25 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 14 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.5 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 15 |
K2HPO4
HO4P.2K 174.174 g/mol
|
1 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 16 |
NaHCO3
CHO3.Na 84.006 g/mol
|
10 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 17 |
NaCl
Cl.Na 58.443 g/mol
|
2 G_PER_L |
CHEBI:26710
sodium chloride
|
Preparation
- Mix components thoroughly, bring to a boil and cool down under a stream of O2-free CO2. While gassing, add the following solutions and adjust pH to 7.2:
- Distribute the medium into culture vessels (e.g., 10 ml in Balch type tubes) under N2, seal with butyl rubber stoppers and autoclave.
- Dissolve 0.1 ml of vitamin K1 in 20 ml 95% ethanol and filter sterilize. Store refrigerated in a brown bottle.
- Dissolve 50 mg hemin first in 1 ml of 1 N NaOH. Then adjust volume to 100 ml.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:19.043282Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J1213 |
| 2026-03-13T03:09:16.289770Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:19:12.882035Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002382 |
| 2026-03-17T06:30:24.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:25.927815+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "PYG MEDIUM (J), MODIFIED" to "pyg_medium_j_modified" for consistent matching |
| 2026-06-05T07:41:43.090203+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 12 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:10.597386+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |