Overview
- External ontology
- mediadive.medium:J1215 (source link unavailable) — MODIFIED HALOALKALIPHILE MEDIUM
- Applications
-
- Microbial cultivation
- High-metal flag
- True
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1215
Composition
flowchart LR
CultureMech_002384["modified_haloalkaliphile_medium"]
ing0_Yeast_extract["Yeast extract"]
CultureMech_002384 --> ing0_Yeast_extract
ing1_Casamino_acids["Casamino acids"]
CultureMech_002384 --> ing1_Casamino_acids
ing2_Na_glutamate["Na glutamate"]
CultureMech_002384 --> ing2_Na_glutamate
CHEBI_64220["CHEBI:64220"]
ing2_Na_glutamate -.-> CHEBI_64220
ing3_Trisodium_citrate_x_2_H2O["Trisodium citrate x 2 H2O"]
CultureMech_002384 --> ing3_Trisodium_citrate_x_2_H2O
CHEBI_53258["CHEBI:53258"]
ing3_Trisodium_citrate_x_2_H2O -.-> CHEBI_53258
ing4_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_002384 --> ing4_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing4_MgSO4_x_7_H2O -.-> CHEBI_31795
ing5_CaSO4_x_2_H2O["CaSO4 x 2 H2O"]
CultureMech_002384 --> ing5_CaSO4_x_2_H2O
CHEBI_32583["CHEBI:32583"]
ing5_CaSO4_x_2_H2O -.-> CHEBI_32583
ing6_KCl["KCl"]
CultureMech_002384 --> ing6_KCl
CHEBI_32588["CHEBI:32588"]
ing6_KCl -.-> CHEBI_32588
ing7_NaCl["NaCl"]
CultureMech_002384 --> ing7_NaCl
CHEBI_26710["CHEBI:26710"]
ing7_NaCl -.-> CHEBI_26710
ing8_Na2CO3["Na2CO3"]
CultureMech_002384 --> ing8_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing8_Na2CO3 -.-> CHEBI_29377
Ingredients (9)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Yeast extract | 2 G_PER_L | ||
| 2 | Casamino acids | 2 G_PER_L | ||
| 3 |
Na glutamate
C5H8NO4.Na 169.112 g/mol
|
1 G_PER_L |
CHEBI:64220
monosodium glutamate
|
|
| 4 |
Trisodium citrate x 2 H2O
C6H5O7.3Na 258.069 g/mol
|
3 G_PER_L |
CHEBI:53258
sodium citrate
|
|
| 5 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.1 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 6 |
CaSO4 x 2 H2O
Ca.2H2O.O4S 172.171 g/mol
|
0.1 G_PER_L |
CHEBI:32583
calcium sulfate dihydrate
|
|
| 7 |
KCl
Cl.K 74.551 g/mol
|
1 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 8 |
NaCl
Cl.Na 58.443 g/mol
|
100.0 G_PER_L |
CHEBI:26710
sodium chloride
|
[Collapsed 2 identical duplicates: 100.0, 100.0] |
| 9 |
Na2CO3
CO3.2Na 105.988 g/mol
|
80 G_PER_L |
CHEBI:29377
sodium carbonate
|
Preparation
- Add components to distilled water and bring volume to 500 ml.
- Add components to distilled water and bring volume to 500 ml.
- Autoclave solutions A and B separately and combine them after cooling. For preparation of solid medium, add 18.0 g agar to Solution A (500 ml), combine them after cooling to 60-70C, mix well and quickly distribute into sterile petri dishes or test tubes.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:19.052897Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J1215 |
| 2026-01-28T19:48:14.765481Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T03:08:52.954982Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:19:12.903816Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002384 |
| 2026-03-17T06:29:57.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:25.951739+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MODIFIED HALOALKALIPHILE MEDIUM" to "modified_haloalkaliphile_medium" for consistent matching |
| 2026-06-05T07:41:36.644825+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 8 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:48.784417+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (audit G21/G22) | Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI). |
| 2026-06-10T07:47:08.649740+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-09-02T07:38:51.732283+00:00 | repair_merged_duplicates.py | REPAIRED_SUMMED_DUPLICATE_MERGE | Collapsed 1 ingredient row(s) whose concentration was the SUM of identical duplicate values written by cleanup_media_quality.py. Every merged part was the same value, so the pre-merge value is unambiguous and no source lookup was needed. Rows whose merged parts differ were left untouched. |