modified_nitrate_mineral_salts_medium_2

CultureMech:002389 bacterial DEFINED LIQUID
Original name: MODIFIED NITRATE MINERAL SALTS MEDIUM-2

Overview

External ontology
mediadive.medium:J1221 (source link unavailable) — MODIFIED NITRATE MINERAL SALTS MEDIUM-2
Applications
  • Microbial cultivation
pH
6.8
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1221

Composition

flowchart LR
    CultureMech_002389["modified_nitrate_mineral_salts_medium_2"]
    ing0_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_002389 --> ing0_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing0_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing1_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_002389 --> ing1_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing1_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing2_KNO3["KNO3"]
    CultureMech_002389 --> ing2_KNO3
    CHEBI_63043["CHEBI:63043"]
    ing2_KNO3 -.-> CHEBI_63043
    ing3_HEPES["HEPES"]
    CultureMech_002389 --> ing3_HEPES
    CHEBI_46756["CHEBI:46756"]
    ing3_HEPES -.-> CHEBI_46756
    ing4_Ammonium_ferric_citrate["Ammonium ferric citrate"]
    CultureMech_002389 --> ing4_Ammonium_ferric_citrate
    CHEBI_31604["CHEBI:31604"]
    ing4_Ammonium_ferric_citrate -.-> CHEBI_31604
    ing5_KH2PO4["KH2PO4"]
    CultureMech_002389 --> ing5_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing5_KH2PO4 -.-> CHEBI_63036
    ing6_Na2HPO4_x_2_H2O["Na2HPO4 x 2 H2O"]
    CultureMech_002389 --> ing6_Na2HPO4_x_2_H2O
    CHEBI_91258["CHEBI:91258"]
    ing6_Na2HPO4_x_2_H2O -.-> CHEBI_91258
    ing7_Vitamin_B12["Vitamin B12"]
    CultureMech_002389 --> ing7_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing7_Vitamin_B12 -.-> CHEBI_176843
    ing8_L_Ascorbic_acid["L-Ascorbic acid"]
    CultureMech_002389 --> ing8_L_Ascorbic_acid
    CHEBI_29073["CHEBI:29073"]
    ing8_L_Ascorbic_acid -.-> CHEBI_29073
    ing9_Nicotinamide["Nicotinamide"]
    CultureMech_002389 --> ing9_Nicotinamide
    CHEBI_17154["CHEBI:17154"]
    ing9_Nicotinamide -.-> CHEBI_17154
    ing10_HCl["HCl"]
    CultureMech_002389 --> ing10_HCl
    CHEBI_17883["CHEBI:17883"]
    ing10_HCl -.-> CHEBI_17883
    ing11_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
    CultureMech_002389 --> ing11_FeCl2_x_4_H2O
    CHEBI_86249["CHEBI:86249"]
    ing11_FeCl2_x_4_H2O -.-> CHEBI_86249
    ing12_ZnCl2["ZnCl2"]
    CultureMech_002389 --> ing12_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing12_ZnCl2 -.-> CHEBI_49976
    ing13_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_002389 --> ing13_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing13_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing14_H3BO3["H3BO3"]
    CultureMech_002389 --> ing14_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing14_H3BO3 -.-> CHEBI_33118
    ing15_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_002389 --> ing15_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing15_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing16_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_002389 --> ing16_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing16_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing17_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_002389 --> ing17_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing17_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing18_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_002389 --> ing18_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing18_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing19_Biotin["Biotin"]
    CultureMech_002389 --> ing19_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing19_Biotin -.-> CHEBI_15956
    ing20_Folic_acid["Folic acid"]
    CultureMech_002389 --> ing20_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing20_Folic_acid -.-> CHEBI_27470
    ing21_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
    CultureMech_002389 --> ing21_Pyridoxine_hydrochloride
    CHEBI_30961["CHEBI:30961"]
    ing21_Pyridoxine_hydrochloride -.-> CHEBI_30961
    ing22_Thiamine_HCl["Thiamine HCl"]
    CultureMech_002389 --> ing22_Thiamine_HCl
    CHEBI_49105["CHEBI:49105"]
    ing22_Thiamine_HCl -.-> CHEBI_49105
    ing23_Riboflavin["Riboflavin"]
    CultureMech_002389 --> ing23_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing23_Riboflavin -.-> CHEBI_17015
    ing24_Nicotinic_acid["Nicotinic acid"]
    CultureMech_002389 --> ing24_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing24_Nicotinic_acid -.-> CHEBI_15940
    ing25_Calcium_pantothenate["Calcium pantothenate"]
    CultureMech_002389 --> ing25_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing25_Calcium_pantothenate -.-> CHEBI_31345
    ing26_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_002389 --> ing26_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing26_p_Aminobenzoic_acid -.-> CHEBI_30753
    ing27_Lipoic_acid["Lipoic acid"]
    CultureMech_002389 --> ing27_Lipoic_acid
    CHEBI_16494["CHEBI:16494"]
    ing27_Lipoic_acid -.-> CHEBI_16494

Ingredients (28)

# Ingredient Concentration Ontology Notes
1 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.984252 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
2 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.19685 G_PER_L CHEBI:86158
calcium chloride dihydrate
3 KNO3
K.NO3 101.102 g/mol
0.246063 G_PER_L CHEBI:63043
potassium nitrate
4 HEPES 2 G_PER_L CHEBI:46756
HEPES
5 Ammonium ferric citrate 0.00393701 G_PER_L CHEBI:31604
ferric ammonium citrate
6 KH2PO4
H2O4P.K 136.084 g/mol
14 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
7 Na2HPO4 x 2 H2O
2H2O.HO4P.2Na 177.988 g/mol
36 G_PER_L CHEBI:91258
disodium hydrogenphosphate dihydrate
8 Vitamin B12 0.0201 G_PER_L CHEBI:176843
vitamin B12
 [Merged 2 duplicates: 0.02, 0.0001]
9 L-Ascorbic acid
C6H8O6 176.124 g/mol
0.005 G_PER_L CHEBI:29073
L-ascorbic acid
10 Nicotinamide
C6H6N2O 122.127 g/mol
0.005 G_PER_L CHEBI:17154
nicotinamide
11 HCl
HCl 36.461 g/mol
2.5 G_PER_L CHEBI:17883
hydrogen chloride
12 FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
1.5 G_PER_L CHEBI:86249
iron dichloride tetrahydrate
13 ZnCl2
2Cl.Zn 136.296 g/mol
0.07 G_PER_L CHEBI:49976
zinc dichloride
14 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.1 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
15 H3BO3
H3BO3 61.833 g/mol
0.006 G_PER_L CHEBI:33118
boric acid
16 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.19 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
17 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.002 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
18 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.024 G_PER_L CHEBI:34887
nickel dichloride
19 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.036 G_PER_L CHEBI:75213
sodium molybdate dihydrate
20 Biotin
C10H16N2O3S 244.316 g/mol
0.002 G_PER_L CHEBI:15956
biotin
21 Folic acid
C19H19N7O6 441.404 g/mol
0.002 G_PER_L CHEBI:27470
folic acid
22 Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
0.01 G_PER_L CHEBI:30961
pyridoxine hydrochloride
23 Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
0.005 G_PER_L CHEBI:49105
thiamine hydrochloride
24 Riboflavin
C17H20N4O6 376.369 g/mol
0.005 G_PER_L CHEBI:17015
riboflavin
25 Nicotinic acid
C6H5NO2 123.111 g/mol
0.005 G_PER_L CHEBI:15940
nicotinic acid
26 Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.005 G_PER_L CHEBI:31345
Calcium pantothenate
27 p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
0.005 G_PER_L CHEBI:30753
4-aminobenzoic acid
28 Lipoic acid
C8H14O2S2 206.332 g/mol
0.005 G_PER_L CHEBI:16494
lipoic acid

Preparation

  1. Mix components thoroughly and autoclave. After cooling, add the following solutions (autoclaved) to the medium:
  2. Adjust pH to 6.8, if necessary. Cultivate with methane or methanol as a substrate as described below:
  3. Methane: Aseptically distribute the medium into sterile serum bottles (leave &; 80% gas phase by volume) and seal with rubber stoppers. Replace the gas phase with an air-methane gas mixture (75:25, v/v). Cultivate with shaking.
  4. Methanol: Aseptically distribute the medium into culture vessels (e.g., 20 ml in 60 ml serum bottles), supplement with 4.0 ml per liter filter-sterilized methanol and seal with rubber stoppers.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:19.072988Z mediadive-import Imported from MediaDive Source: JCM, ID: J1221
2026-03-13T03:09:14.102215Z data-quality-cleanup-v1.0 Data quality fixes Merged 1 duplicate ingredient(s)
2026-03-13T06:19:12.939977Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002389
2026-03-17T06:30:21.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:25.992325+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "MODIFIED NITRATE MINERAL SALTS MEDIUM-2" to "modified_nitrate_mineral_salts_medium_2" for consistent matching
2026-06-05T07:41:37.500537+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 27 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:37:35.638947+00:00 primary-term-regrounding-v1.0 Re-grounded wrong primary term id (OAK-verified) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-06-10T07:47:08.873537+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.