Overview
- External ontology
- mediadive.medium:J1231 (source link unavailable) — HLH MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 8.0
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1231
Composition
flowchart LR
CultureMech_002398["hlh_medium"]
ing0_Na2CO3["Na2CO3"]
CultureMech_002398 --> ing0_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing0_Na2CO3 -.-> CHEBI_29377
ing1_K2HPO4["K2HPO4"]
CultureMech_002398 --> ing1_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing1_K2HPO4 -.-> CHEBI_131527
ing2_TES["TES"]
CultureMech_002398 --> ing2_TES
CHEBI_39035["CHEBI:39035"]
ing2_TES -.-> CHEBI_39035
ing3_NH4Cl["NH4Cl"]
CultureMech_002398 --> ing3_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing3_NH4Cl -.-> CHEBI_31206
ing4_Ammonium_ferric_citrate["Ammonium ferric citrate"]
CultureMech_002398 --> ing4_Ammonium_ferric_citrate
CHEBI_31604["CHEBI:31604"]
ing4_Ammonium_ferric_citrate -.-> CHEBI_31604
ing5_NaHCO3["NaHCO3"]
CultureMech_002398 --> ing5_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing5_NaHCO3 -.-> CHEBI_32139
ing6_Yeast_extract["Yeast extract"]
CultureMech_002398 --> ing6_Yeast_extract
ing7_NaNO3["NaNO3"]
CultureMech_002398 --> ing7_NaNO3
CHEBI_63005["CHEBI:63005"]
ing7_NaNO3 -.-> CHEBI_63005
ing8_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_002398 --> ing8_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing8_MgSO4_x_7_H2O -.-> CHEBI_31795
ing9_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_002398 --> ing9_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing9_CaCl2_x_2_H2O -.-> CHEBI_86158
ing10_Na2_EDTA["Na2-EDTA"]
CultureMech_002398 --> ing10_Na2_EDTA
CHEBI_64734["CHEBI:64734"]
ing10_Na2_EDTA -.-> CHEBI_64734
ing11_H3BO3["H3BO3"]
CultureMech_002398 --> ing11_H3BO3
CHEBI_33118["CHEBI:33118"]
ing11_H3BO3 -.-> CHEBI_33118
ing12_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_002398 --> ing12_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing12_MnCl2_x_4_H2O -.-> CHEBI_86368
ing13_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_002398 --> ing13_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing13_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing14_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_002398 --> ing14_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing14_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing15_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_002398 --> ing15_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing15_CuSO4_x_5_H2O -.-> CHEBI_31440
ing16_Co_NO3_2_x_6_H2O["Co(NO3)2 x 6 H2O"]
CultureMech_002398 --> ing16_Co_NO3_2_x_6_H2O
CHEBI_86214["CHEBI:86214"]
ing16_Co_NO3_2_x_6_H2O -.-> CHEBI_86214
Ingredients (17)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Na2CO3
CO3.2Na 105.988 g/mol
|
0.0297199 G_PER_L |
CHEBI:29377
sodium carbonate
|
|
| 2 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.0371498 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 3 | TES | 3.04507 G_PER_L |
CHEBI:39035
TES
|
|
| 4 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.365408 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 5 | Ammonium ferric citrate | 1 G_PER_L |
CHEBI:31604
ferric ammonium citrate
|
|
| 6 |
NaHCO3
CHO3.Na 84.006 g/mol
|
10 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 7 | Yeast extract | 10 G_PER_L | ||
| 8 |
NaNO3
NO3.Na 84.994 g/mol
|
136 G_PER_L |
CHEBI:63005
sodium nitrate
|
|
| 9 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
6.81818 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 10 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
3.27273 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 11 |
Na2-EDTA
C10H14N2O8.2Na 336.208 g/mol
|
0.0909091 G_PER_L |
CHEBI:64734
EDTA disodium salt (anhydrous)
|
|
| 12 |
H3BO3
H3BO3 61.833 g/mol
|
2.9 G_PER_L |
CHEBI:33118
boric acid
|
|
| 13 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
1.8 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 14 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.222 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 15 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.39 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 16 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.079 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 17 |
Co(NO3)2 x 6 H2O
Co.6H2O.2NO3 291.031 g/mol
|
0.0494 G_PER_L |
CHEBI:86214
cobalt dinitrate hexahydrate
|
Preparation
- Adjust pH to 8.0.
- After autoclaving, add per liter the following solutions (autoclaved or *filter-sterilized):
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:19.109469Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J1231 |
| 2026-03-13T06:19:13.008651Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002398 |
| 2026-03-17T06:30:39.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:26.068535+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "HLH MEDIUM" to "hlh_medium" for consistent matching |
| 2026-06-05T07:41:08.591118+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 15 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:59.788496+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |