Overview
- External ontology
- mediadive.medium:J1233 (source link unavailable) — CM+YE MEDIUM (pH 9)
- Applications
-
- Microbial cultivation
- pH
- 9.0
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1233
Composition
flowchart LR
CultureMech_002400["cm_ye_medium_ph_9"]
ing0_Casamino_acids["Casamino acids"]
CultureMech_002400 --> ing0_Casamino_acids
ing1_Yeast_extract["Yeast extract"]
CultureMech_002400 --> ing1_Yeast_extract
ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_002400 --> ing2_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
ing3_Trisodium_citrate_x_2_H2O["Trisodium citrate x 2 H2O"]
CultureMech_002400 --> ing3_Trisodium_citrate_x_2_H2O
CHEBI_53258["CHEBI:53258"]
ing3_Trisodium_citrate_x_2_H2O -.-> CHEBI_53258
ing4_KCl["KCl"]
CultureMech_002400 --> ing4_KCl
CHEBI_32588["CHEBI:32588"]
ing4_KCl -.-> CHEBI_32588
ing5_NaCl["NaCl"]
CultureMech_002400 --> ing5_NaCl
CHEBI_26710["CHEBI:26710"]
ing5_NaCl -.-> CHEBI_26710
ing6_Agar["Agar"]
CultureMech_002400 --> ing6_Agar
CHEBI_2509["CHEBI:2509"]
ing6_Agar -.-> CHEBI_2509
ing7_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_002400 --> ing7_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing7_FeSO4_x_7_H2O -.-> CHEBI_75836
Ingredients (8)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Casamino acids | 7.49251 G_PER_L | ( vitamin assay) | Copied from referenced JCM Medium 59 | |
| 2 | Yeast extract | 9.99001 G_PER_L | Copied from referenced JCM Medium 59 | |
| 3 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
19.98 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Copied from referenced JCM Medium 59 |
| 4 |
Trisodium citrate x 2 H2O
C6H5O7.3Na 258.069 g/mol
|
2.997 G_PER_L |
CHEBI:53258
sodium citrate
|
Copied from referenced JCM Medium 59 |
| 5 |
KCl
Cl.K 74.551 g/mol
|
1.998 G_PER_L |
CHEBI:32588
potassium chloride
|
Copied from referenced JCM Medium 59 |
| 6 |
NaCl
Cl.Na 58.443 g/mol
|
199.8 G_PER_L |
CHEBI:26710
sodium chloride
|
Copied from referenced JCM Medium 59 |
| 7 | Agar | 14.985 G_PER_L |
CHEBI:2509
agar
|
Copied from referenced JCM Medium 59 |
| 8 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
49.8 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
Copied from referenced JCM Medium 59 |
Preparation
- Prepare CM+YE medium (see Medium No. 59). After autoclaving, adjust pH to 9.0 with sterilized 10% Na2CO3 solution.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:19.115906Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J1233 |
| 2026-02-03T11:26:53.525029 | quality-tagger-v1.0 | Added data quality flag | Marked as incomplete_composition due to placeholder ingredients |
| 2026-03-13T06:19:13.015911Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002400 |
| 2026-03-17T19:45:26.076420+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "CM+YE MEDIUM (pH 9)" to "cm_ye_medium_ph_9" for consistent matching |
| 2026-04-04T21:48:41.192458 | resolve_option_c_references | RESOLVED_REFERENCE | Resolved reference by copying from cm_ye_medium (CultureMech:002947) |
| 2026-04-04T21:53:21.893906 | resolve_option_c_references | RESOLVED_REFERENCE | Resolved reference by copying from cm_ye_medium (CultureMech:002947) |
| 2026-05-16T08:57:26.643666+00:00 | migrate_data_quality_flags.py | MIGRATED_DATA_QUALITY_FLAGS | dict(2 keys) -> list(1 entries) |
| 2026-06-05T07:40:54.552437+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:36.416604+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (audit G21/G22) | Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI). |