r2a_agar_with_fumarate

CultureMech:002402 bacterial COMPLEX SOLID_AGAR
Original name: R2A AGAR WITH FUMARATE

Overview

External ontology
mediadive.medium:J1235 (source link unavailable) — R2A AGAR WITH FUMARATE
Applications
  • Microbial cultivation
Notes
JCM Medium 1235 lists 18.2 g/L R2A agar (BD-Difco), 3.2 g/L disodium fumarate, and 1.0 L distilled water; its comment describes replacing the gas phase with N2 or using AnaeroPack(TM) for anaerobic cultivation.

Composition

flowchart LR
    CultureMech_002402["r2a_agar_with_fumarate"]
    ing0_Disodium_fumarate["Disodium fumarate"]
    CultureMech_002402 --> ing0_Disodium_fumarate
    CHEBI_115156["CHEBI:115156"]
    ing0_Disodium_fumarate -.-> CHEBI_115156

Ingredients (1)

# Ingredient Concentration Ontology Notes
1 Disodium fumarate
C4H2O4.2Na 160.036 g/mol
3.2 G_PER_L CHEBI:115156
disodium fumarate
 Adds 3.2 g/L disodium fumarate to R2A Agar.

Preparation

  1. Adds 3.2 g/L disodium fumarate to R2A Agar.
  2. Autoclave at 121 C for 15 min.
  3. For anaerobic cultivation, replace the gas phase with N2 or use AnaeroPack(TM).

Solutions

R2A Agar

Dose: 1000 ML_PER_L

CultureMech:002706

Adds 3.2 g/L disodium fumarate to R2A Agar.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:19.119198Z mediadive-import Imported from MediaDive Source: JCM, ID: J1235
2026-03-13T06:19:13.020936Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002402
2026-03-17T06:30:40.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:26.082198+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "R2A AGAR WITH FUMARATE" to "r2a_agar_with_fumarate" for consistent matching
2026-06-05T07:41:43.590508+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 2 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-09-08T00:00:00-07:00 repair_jcm_r2a_score20.py RESOLVED_JCM_R2A_SCORE20 Adds 3.2 g/L disodium fumarate to R2A Agar.; For anaerobic cultivation, replace the gas phase with N2 or use AnaeroPack(TM).