briggs_liver_broth_ph_5_0

CultureMech:002406 bacterial COMPLEX LIQUID
Original name: BRIGGS LIVER BROTH (pH 5.0)

Overview

External ontology
mediadive.medium:J123 (source link unavailable) — BRIGGS LIVER BROTH (pH 5.0)
Applications
  • Microbial cultivation
pH
7.0
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=123

Composition

flowchart LR
    CultureMech_002406["briggs_liver_broth_ph_5_0"]
    ing0_Tomato_juice["Tomato juice"]
    CultureMech_002406 --> ing0_Tomato_juice
    ing1_Neopeptone["Neopeptone"]
    CultureMech_002406 --> ing1_Neopeptone
    ing2_Yeast_extract["Yeast extract"]
    CultureMech_002406 --> ing2_Yeast_extract
    ing3_Liver_extract["Liver extract"]
    CultureMech_002406 --> ing3_Liver_extract
    ing4_Glucose["Glucose"]
    CultureMech_002406 --> ing4_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing4_Glucose -.-> CHEBI_17234
    ing5_Starch["Starch"]
    CultureMech_002406 --> ing5_Starch
    CHEBI_28017["CHEBI:28017"]
    ing5_Starch -.-> CHEBI_28017
    ing6_NaCl["NaCl"]
    CultureMech_002406 --> ing6_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing6_NaCl -.-> CHEBI_26710
    ing7_Tween_80["Tween 80"]
    CultureMech_002406 --> ing7_Tween_80
    CHEBI_53426["CHEBI:53426"]
    ing7_Tween_80 -.-> CHEBI_53426
    ing8_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
    CultureMech_002406 --> ing8_L_Cysteine_HCl_x_H2O
    CHEBI_91248["CHEBI:91248"]
    ing8_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248

Ingredients (9)

# Ingredient Concentration Ontology Notes
1 Tomato juice 400 G_PER_L
2 Neopeptone 15 G_PER_L
3 Yeast extract 6 G_PER_L
4 Liver extract 75 G_PER_L
5 Glucose
C6H12O6 180.156 g/mol
20 G_PER_L CHEBI:17234
glucose
6 Starch 0.5 G_PER_L CHEBI:28017
starch
7 NaCl
Cl.Na 58.443 g/mol
5 G_PER_L CHEBI:26710
sodium chloride
8 Tween 80
(C2H4O)w.(C2H4O)x.(C2H4O)y.(C2H4O)z.C24H44O6 604.822 g/mol
1 G_PER_L CHEBI:53426
polysorbate 80
9 L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
0.2 G_PER_L CHEBI:91248
L-cysteine hydrochloride hydrate

Preparation

  1. Adjust pH to 5.0.
  2. Dilute Tomato juice (Del Monte) with an equal volume of distilled water, adjust pH to 7.0 with 10% NaOH and filter with filter paper.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:15.767281Z mediadive-import Imported from MediaDive Source: JCM, ID: J123
2026-03-13T06:19:13.045915Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002406
2026-03-17T06:30:13.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:26.110132+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "BRIGGS LIVER BROTH (pH 5.0)" to "briggs_liver_broth_ph_5_0" for consistent matching
2026-06-05T07:40:51.551454+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-10-07T14:33:27.670564+00:00 codex CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged.