bicarbonate_buffered_medium_with_acethylene

CultureMech:002412 bacterial DEFINED LIQUID
Original name: BICARBONATE-BUFFERED MEDIUM WITH ACETHYLENE

Overview

External ontology
mediadive.medium:J1246 (source link unavailable) — BICARBONATE-BUFFERED MEDIUM WITH ACETHYLENE
Applications
  • Microbial cultivation
pH
7.5
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1246

Composition

flowchart LR
    CultureMech_002412["bicarbonate_buffered_medium_with_acethylene"]
    ing0_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_002412 --> ing0_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing0_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing1_KCl["KCl"]
    CultureMech_002412 --> ing1_KCl
    CHEBI_32588["CHEBI:32588"]
    ing1_KCl -.-> CHEBI_32588
    ing2_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_002412 --> ing2_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing2_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing3_KH2PO4["KH2PO4"]
    CultureMech_002412 --> ing3_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing3_KH2PO4 -.-> CHEBI_63036
    ing4_FeSO4_x_6_H2O["FeSO4 x 6 H2O"]
    CultureMech_002412 --> ing4_FeSO4_x_6_H2O
    CHEBI_75832["CHEBI:75832"]
    ing4_FeSO4_x_6_H2O -.-> CHEBI_75832
    ing5_NaCl["NaCl"]
    CultureMech_002412 --> ing5_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing5_NaCl -.-> CHEBI_26710
    ing6_NH4Cl["NH4Cl"]
    CultureMech_002412 --> ing6_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing6_NH4Cl -.-> CHEBI_31206
    ing7_HEPES["HEPES"]
    CultureMech_002412 --> ing7_HEPES
    CHEBI_46756["CHEBI:46756"]
    ing7_HEPES -.-> CHEBI_46756
    ing8_NaHCO3["NaHCO3"]
    CultureMech_002412 --> ing8_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing8_NaHCO3 -.-> CHEBI_32139
    ing9_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
    CultureMech_002412 --> ing9_L_Cysteine_HCl_x_H2O
    CHEBI_91248["CHEBI:91248"]
    ing9_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
    ing10_HCl["HCl"]
    CultureMech_002412 --> ing10_HCl
    CHEBI_17883["CHEBI:17883"]
    ing10_HCl -.-> CHEBI_17883
    ing11_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
    CultureMech_002412 --> ing11_FeCl2_x_4_H2O
    CHEBI_86249["CHEBI:86249"]
    ing11_FeCl2_x_4_H2O -.-> CHEBI_86249
    ing12_ZnCl2["ZnCl2"]
    CultureMech_002412 --> ing12_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing12_ZnCl2 -.-> CHEBI_49976
    ing13_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_002412 --> ing13_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing13_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing14_H3BO3["H3BO3"]
    CultureMech_002412 --> ing14_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing14_H3BO3 -.-> CHEBI_33118
    ing15_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_002412 --> ing15_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing15_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing16_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_002412 --> ing16_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing16_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing17_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_002412 --> ing17_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing17_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing18_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_002412 --> ing18_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing18_Na2MoO4_x_2_H2O -.-> CHEBI_75213

Ingredients (19)

# Ingredient Concentration Ontology Notes
1 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.219085 G_PER_L CHEBI:86158
calcium chloride dihydrate
2 KCl
Cl.K 74.551 g/mol
0.194742 G_PER_L CHEBI:32588
potassium chloride
3 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
0.194742 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
4 KH2PO4
H2O4P.K 136.084 g/mol
0.0194742 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
5 FeSO4 x 6 H2O
Fe.O4S 151.908 g/mol
0.1 G_PER_L CHEBI:75832
iron(2+) sulfate (anhydrous)
6 NaCl
Cl.Na 58.443 g/mol
19.4742 G_PER_L CHEBI:26710
sodium chloride
7 NH4Cl
Cl.H4N 53.492 g/mol
0.194742 G_PER_L CHEBI:31206
ammonium chloride
8 HEPES 5.06329 G_PER_L CHEBI:46756
HEPES
9 NaHCO3
CHO3.Na 84.006 g/mol
20 G_PER_L CHEBI:32139
sodium hydrogencarbonate
10 L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
5 G_PER_L CHEBI:91248
L-cysteine hydrochloride hydrate
11 HCl
HCl 36.461 g/mol
2.5 G_PER_L CHEBI:17883
hydrogen chloride
12 FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
1.5 G_PER_L CHEBI:86249
iron dichloride tetrahydrate
13 ZnCl2
2Cl.Zn 136.296 g/mol
0.07 G_PER_L CHEBI:49976
zinc dichloride
14 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.1 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
15 H3BO3
H3BO3 61.833 g/mol
0.006 G_PER_L CHEBI:33118
boric acid
16 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.19 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
17 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.002 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
18 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.024 G_PER_L CHEBI:34887
nickel dichloride
19 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.036 G_PER_L CHEBI:75213
sodium molybdate dihydrate

Preparation

  1. Mix components thoroughly and adjust pH to 7.5. Distribute the medium in culture vessels (e.g., 10 ml in Balch-type tubes), flush with N2 and seal with butyl rubber stoppers. Autoclave. After cooling, add per liter the following solution (autoclaved or *filter-sterilized):
  2. After inoculation, add sterile pure acetylene gas, made from calcium carbide and water, to the gas phase (the first time, 0.5% volume of the gas phase: the next day, 1.0% volume: increase each day until 2.5% volume is reached).

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:19.155354Z mediadive-import Imported from MediaDive Source: JCM, ID: J1246
2026-03-13T06:19:13.092562Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002412
2026-03-17T06:29:47.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:26.162372+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "BICARBONATE-BUFFERED MEDIUM WITH ACETHYLENE" to "bicarbonate_buffered_medium_with_acethylene" for consistent matching
2026-06-05T07:40:50.983684+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 19 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:53.903132+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.