Overview
- External ontology
- mediadive.medium:J1247 (source link unavailable) — R2A MEDIUM WITH 25% SALT
- Applications
-
- Microbial cultivation
- pH
- 7.5
- High-metal flag
- True
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1247
Composition
flowchart LR
CultureMech_002413["r2a_medium_with_25_salt"]
ing0_Yeast_extract["Yeast extract"]
CultureMech_002413 --> ing0_Yeast_extract
ing1_Proteose_peptone_no__3["Proteose peptone no. 3"]
CultureMech_002413 --> ing1_Proteose_peptone_no__3
ing2_Casamino_acids["Casamino acids"]
CultureMech_002413 --> ing2_Casamino_acids
ing3_Glucose["Glucose"]
CultureMech_002413 --> ing3_Glucose
CHEBI_17234["CHEBI:17234"]
ing3_Glucose -.-> CHEBI_17234
ing4_Starch["Starch"]
CultureMech_002413 --> ing4_Starch
CHEBI_28017["CHEBI:28017"]
ing4_Starch -.-> CHEBI_28017
ing5_Sodium_pyruvate["Sodium pyruvate"]
CultureMech_002413 --> ing5_Sodium_pyruvate
CHEBI_50144["CHEBI:50144"]
ing5_Sodium_pyruvate -.-> CHEBI_50144
ing6_K2HPO4["K2HPO4"]
CultureMech_002413 --> ing6_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing6_K2HPO4 -.-> CHEBI_131527
ing7_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_002413 --> ing7_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing7_MgSO4_x_7_H2O -.-> CHEBI_31795
ing8_Sea_water["Sea water"]
CultureMech_002413 --> ing8_Sea_water
ing9_NaCl["NaCl"]
CultureMech_002413 --> ing9_NaCl
CHEBI_26710["CHEBI:26710"]
ing9_NaCl -.-> CHEBI_26710
ing10_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_002413 --> ing10_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing10_MgCl2_x_6_H2O -.-> CHEBI_86345
ing11_KCl["KCl"]
CultureMech_002413 --> ing11_KCl
CHEBI_32588["CHEBI:32588"]
ing11_KCl -.-> CHEBI_32588
ing12_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_002413 --> ing12_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing12_CaCl2_x_2_H2O -.-> CHEBI_86158
ing13_NaBr["NaBr"]
CultureMech_002413 --> ing13_NaBr
CHEBI_63004["CHEBI:63004"]
ing13_NaBr -.-> CHEBI_63004
ing14_NaHCO3["NaHCO3"]
CultureMech_002413 --> ing14_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing14_NaHCO3 -.-> CHEBI_32139
Ingredients (15)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Yeast extract | 0.5 G_PER_L | ||
| 2 | Proteose peptone no. 3 | 0.5 G_PER_L | ||
| 3 | Casamino acids | 0.5 G_PER_L | ||
| 4 |
Glucose
C6H12O6 180.156 g/mol
|
0.5 G_PER_L |
CHEBI:17234
glucose
|
|
| 5 | Starch | 0.5 G_PER_L |
CHEBI:28017
starch
|
|
| 6 |
Sodium pyruvate
C3H3O3.Na 110.044 g/mol
|
0.3 G_PER_L |
CHEBI:50144
sodium pyruvate
|
|
| 7 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.3 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 8 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
61.05 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
[Merged 2 duplicates: 0.05, 61.0] |
| 9 | Sea water | 833 G_PER_L | ||
| 10 |
NaCl
Cl.Na 58.443 g/mol
|
234 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 11 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
39 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 12 |
KCl
Cl.K 74.551 g/mol
|
6 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 13 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
1.3 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 14 |
NaBr
Br.Na 102.894 g/mol
|
0.7 G_PER_L |
CHEBI:63004
sodium bromide
|
|
| 15 |
NaHCO3
CHO3.Na 84.006 g/mol
|
0.2 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
Preparation
- Adjust pH to 7.5.
- 30% <I>Seawater solution:</I>
- Add components to distilled water and bring volume to 1.0 L.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:19.159514Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J1247 |
| 2026-01-28T19:48:10.982393Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T03:08:45.607803Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:19:13.100858Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002413 |
| 2026-03-17T06:29:48.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:26.171601+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "R2A MEDIUM WITH 25% SALT" to "r2a_medium_with_25_salt" for consistent matching |
| 2026-06-05T07:41:43.642054+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 12 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:50.023560+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (audit G21/G22) | Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI). |
| 2026-06-10T07:47:10.785764+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |