salicibibacter_medium

CultureMech:002419 bacterial COMPLEX SOLID_AGAR
Original name: SALICIBIBACTER MEDIUM

Overview

External ontology
mediadive.medium:J1253 (source link unavailable) — SALICIBIBACTER MEDIUM
Applications
  • Microbial cultivation
pH
7.8
High-metal flag
True
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1253

Composition

flowchart LR
    CultureMech_002419["salicibibacter_medium"]
    ing0_KH2PO4["KH2PO4"]
    CultureMech_002419 --> ing0_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing0_KH2PO4 -.-> CHEBI_63036
    ing1_KCl["KCl"]
    CultureMech_002419 --> ing1_KCl
    CHEBI_32588["CHEBI:32588"]
    ing1_KCl -.-> CHEBI_32588
    ing2_NH4Cl["NH4Cl"]
    CultureMech_002419 --> ing2_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing2_NH4Cl -.-> CHEBI_31206
    ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_002419 --> ing3_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing4_CaSO4_x_2_H2O["CaSO4 x 2 H2O"]
    CultureMech_002419 --> ing4_CaSO4_x_2_H2O
    CHEBI_32583["CHEBI:32583"]
    ing4_CaSO4_x_2_H2O -.-> CHEBI_32583
    ing5_NaCl["NaCl"]
    CultureMech_002419 --> ing5_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing5_NaCl -.-> CHEBI_26710
    ing6_Na_glutamate["Na glutamate"]
    CultureMech_002419 --> ing6_Na_glutamate
    CHEBI_64220["CHEBI:64220"]
    ing6_Na_glutamate -.-> CHEBI_64220
    ing7_Yeast_extract["Yeast extract"]
    CultureMech_002419 --> ing7_Yeast_extract
    ing8_Casamino_acids["Casamino acids"]
    CultureMech_002419 --> ing8_Casamino_acids
    ing9_Agar["Agar"]
    CultureMech_002419 --> ing9_Agar
    CHEBI_2509["CHEBI:2509"]
    ing9_Agar -.-> CHEBI_2509
    ing10_Na2CO3["Na2CO3"]
    CultureMech_002419 --> ing10_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing10_Na2CO3 -.-> CHEBI_29377
    ing11_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_002419 --> ing11_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing11_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing12_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_002419 --> ing12_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing12_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing13_H3BO3["H3BO3"]
    CultureMech_002419 --> ing13_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing13_H3BO3 -.-> CHEBI_33118
    ing14_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_002419 --> ing14_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing14_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing15_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_002419 --> ing15_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing15_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing16_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_002419 --> ing16_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing16_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing17_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_002419 --> ing17_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing17_Na2MoO4_x_2_H2O -.-> CHEBI_75213

Ingredients (18)

# Ingredient Concentration Ontology Notes
1 KH2PO4
H2O4P.K 136.084 g/mol
166.667 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
2 KCl
Cl.K 74.551 g/mol
166.667 G_PER_L CHEBI:32588
potassium chloride
3 NH4Cl
Cl.H4N 53.492 g/mol
166.667 G_PER_L CHEBI:31206
ammonium chloride
4 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
40 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
5 CaSO4 x 2 H2O
Ca.2H2O.O4S 172.171 g/mol
28.3333 G_PER_L CHEBI:32583
calcium sulfate dihydrate
6 NaCl
Cl.Na 58.443 g/mol
23333.3 G_PER_L CHEBI:26710
sodium chloride
7 Na glutamate
C5H8NO4.Na 169.112 g/mol
166.667 G_PER_L CHEBI:64220
monosodium glutamate
8 Yeast extract 833.333 G_PER_L
9 Casamino acids 833.333 G_PER_L
10 Agar 3333.33 G_PER_L CHEBI:2509
agar
11 Na2CO3
CO3.2Na 105.988 g/mol
5 G_PER_L CHEBI:29377
sodium carbonate
12 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.1 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
13 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.03 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
14 H3BO3
H3BO3 61.833 g/mol
0.3 G_PER_L CHEBI:33118
boric acid
15 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.2 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
16 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.01 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
17 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.02 G_PER_L CHEBI:34887
nickel dichloride
18 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.03 G_PER_L CHEBI:75213
sodium molybdate dihydrate

Preparation

  1. Add components to distilled water and bring volume to 1 L. Adjust pH to 6.5 and autoclave. Aseptically add the following solution (autoclaved) to the medium:
  2. Check final pH of the medium to be 7.5-8.0.
  3. Adjust pH to 3.6.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:19.181733Z mediadive-import Imported from MediaDive Source: JCM, ID: J1253
2026-01-28T19:48:18.732379Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:19:13.158103Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002419
2026-03-17T06:30:05.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:26.235480+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "SALICIBIBACTER MEDIUM" to "salicibibacter_medium" for consistent matching
2026-06-05T07:41:45.022448+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 17 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-08T23:19:50.311476+00:00 chebi-regrounding-v1.0 Corrected CHEBI ingredient grounding (shared-id regrounding audit, G21/G22/G24) Part of the shared-id CHEBI regrounding audit (G21/G22/G24): one or more ingredient groundings on this record that had been collapsed onto a chemically non-specific or shared CHEBI id were re-grounded to the correct chemically-specific term, verified against OAK chebi.db rdfs:label. See reports/chebi_grounding_audit.md.
2026-06-10T07:47:11.224617+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.