haloarchaeal_complex_medium_ph_9_0

CultureMech:002420 archaea COMPLEX LIQUID
Original name: HALOARCHAEAL COMPLEX MEDIUM PH 9.0

Overview

External ontology
mediadive.medium:J1254 (source link unavailable) — HALOARCHAEAL COMPLEX MEDIUM PH 9.0
Applications
  • Microbial cultivation
pH
9.0
High-metal flag
True
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1254

Composition

flowchart LR
    CultureMech_002420["haloarchaeal_complex_medium_ph_9_0"]
    ing0_Yeast_extract["Yeast extract"]
    CultureMech_002420 --> ing0_Yeast_extract
    ing1_Fish_peptone["Fish peptone"]
    CultureMech_002420 --> ing1_Fish_peptone
    ing2_Sodium_formate["Sodium formate"]
    CultureMech_002420 --> ing2_Sodium_formate
    CHEBI_62965["CHEBI:62965"]
    ing2_Sodium_formate -.-> CHEBI_62965
    ing3_Sodium_acetate["Sodium acetate"]
    CultureMech_002420 --> ing3_Sodium_acetate
    CHEBI_32954["CHEBI:32954"]
    ing3_Sodium_acetate -.-> CHEBI_32954
    ing4_Sodium_lactate["Sodium lactate"]
    CultureMech_002420 --> ing4_Sodium_lactate
    CHEBI_75228["CHEBI:75228"]
    ing4_Sodium_lactate -.-> CHEBI_75228
    ing5_Sodium_pyruvate["Sodium pyruvate"]
    CultureMech_002420 --> ing5_Sodium_pyruvate
    CHEBI_50144["CHEBI:50144"]
    ing5_Sodium_pyruvate -.-> CHEBI_50144
    ing6_Sodium_succinate["Sodium succinate"]
    CultureMech_002420 --> ing6_Sodium_succinate
    CHEBI_63675["CHEBI:63675"]
    ing6_Sodium_succinate -.-> CHEBI_63675
    ing7_Sodium_malate["Sodium malate"]
    CultureMech_002420 --> ing7_Sodium_malate
    CHEBI_91260["CHEBI:91260"]
    ing7_Sodium_malate -.-> CHEBI_91260
    ing8_Sodium_fumarate["Sodium fumarate"]
    CultureMech_002420 --> ing8_Sodium_fumarate
    CHEBI_115156["CHEBI:115156"]
    ing8_Sodium_fumarate -.-> CHEBI_115156
    ing9_Sodium_citrate["Sodium citrate"]
    CultureMech_002420 --> ing9_Sodium_citrate
    CHEBI_53258["CHEBI:53258"]
    ing9_Sodium_citrate -.-> CHEBI_53258
    ing10_KCl["KCl"]
    CultureMech_002420 --> ing10_KCl
    CHEBI_32588["CHEBI:32588"]
    ing10_KCl -.-> CHEBI_32588
    ing11_K2HPO4["K2HPO4"]
    CultureMech_002420 --> ing11_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing11_K2HPO4 -.-> CHEBI_131527
    ing12_CaCl2_x_H2O["CaCl2 x H2O"]
    CultureMech_002420 --> ing12_CaCl2_x_H2O
    CHEBI_3312["CHEBI:3312"]
    ing12_CaCl2_x_H2O -.-> CHEBI_3312
    ing13_NaNO3["NaNO3"]
    CultureMech_002420 --> ing13_NaNO3
    CHEBI_63005["CHEBI:63005"]
    ing13_NaNO3 -.-> CHEBI_63005
    ing14_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_002420 --> ing14_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing14_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing15_NaCl["NaCl"]
    CultureMech_002420 --> ing15_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing15_NaCl -.-> CHEBI_26710

Ingredients (16)

# Ingredient Concentration Ontology Notes
1 Yeast extract 0.05 G_PER_L
2 Fish peptone 0.25 G_PER_L
3 Sodium formate
CHO2.Na 68.007 g/mol
0.2 G_PER_L CHEBI:62965
sodium formate
4 Sodium acetate
C2H3O2.Na 82.034 g/mol
0.2 G_PER_L CHEBI:32954
sodium acetate
5 Sodium lactate
C3H5O3.Na 112.06 g/mol
0.2 G_PER_L CHEBI:75228
sodium lactate
6 Sodium pyruvate
C3H3O3.Na 110.044 g/mol
0.2 G_PER_L CHEBI:50144
sodium pyruvate
7 Sodium succinate
C4H4O4.2Na 162.052 g/mol
0.2 G_PER_L CHEBI:63675
sodium succinate (anhydrous)
8 Sodium malate 0.2 G_PER_L CHEBI:91260
disodium malate
9 Sodium fumarate
C4H2O4.2Na 160.036 g/mol
0.2 G_PER_L CHEBI:115156
disodium fumarate
10 Sodium citrate
C6H5O7.3Na 258.069 g/mol
0.2 G_PER_L CHEBI:53258
sodium citrate
11 KCl
Cl.K 74.551 g/mol
5.4 G_PER_L CHEBI:32588
potassium chloride
12 K2HPO4
HO4P.2K 174.174 g/mol
0.2 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
13 CaCl2 x H2O
Ca.2Cl 110.984 g/mol
0.1 G_PER_L CHEBI:3312
calcium dichloride
14 NaNO3
NO3.Na 84.994 g/mol
0.5 G_PER_L CHEBI:63005
sodium nitrate
15 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
0.1 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
16 NaCl
Cl.Na 58.443 g/mol
184 G_PER_L CHEBI:26710
sodium chloride

Preparation

  1. Dissolve components in distilled water and bring volume to 1.0 L. For preparation of solid medium, add 20.0 g/L agar (if necessary, use washed agar as described in the recipe of Medium No. 574). After autoclaving, adjust pH to 9.0 by the addition of 10% (w/v) Na2CO3 solution (autoclaved).

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:19.185070Z mediadive-import Imported from MediaDive Source: JCM, ID: J1254
2026-03-13T06:19:13.166109Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002420
2026-03-17T06:30:23.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:26.244618+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "HALOARCHAEAL COMPLEX MEDIUM PH 9.0" to "haloarchaeal_complex_medium_ph_9_0" for consistent matching
2026-06-05T07:41:07.461677+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 13 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:59.429044+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.