lsr1_medium

CultureMech:002426 bacterial DEFINED LIQUID
Original name: LSR1 MEDIUM

Overview

External ontology
mediadive.medium:J1260 (source link unavailable) — LSR1 MEDIUM
Applications
  • Microbial cultivation
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1260

Composition

flowchart LR
    CultureMech_002426["lsr1_medium"]
    ing0_KH2PO4["KH2PO4"]
    CultureMech_002426 --> ing0_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing0_KH2PO4 -.-> CHEBI_63036
    ing1_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_002426 --> ing1_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing1_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing2_NH4Cl["NH4Cl"]
    CultureMech_002426 --> ing2_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing2_NH4Cl -.-> CHEBI_31206
    ing3_K2SO4["K2SO4"]
    CultureMech_002426 --> ing3_K2SO4
    CHEBI_32036["CHEBI:32036"]
    ing3_K2SO4 -.-> CHEBI_32036
    ing4_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_002426 --> ing4_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing4_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing5_FeCl3_x_6_H2O["FeCl3 x 6 H2O"]
    CultureMech_002426 --> ing5_FeCl3_x_6_H2O
    CHEBI_86254["CHEBI:86254"]
    ing5_FeCl3_x_6_H2O -.-> CHEBI_86254
    ing6_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_002426 --> ing6_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing6_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing7_Sodium_Thiosulfate["Sodium Thiosulfate"]
    CultureMech_002426 --> ing7_Sodium_Thiosulfate
    CHEBI_132112["CHEBI:132112"]
    ing7_Sodium_Thiosulfate -.-> CHEBI_132112
    ing8_NaHCO3["NaHCO3"]
    CultureMech_002426 --> ing8_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing8_NaHCO3 -.-> CHEBI_32139
    ing9_Biotin["Biotin"]
    CultureMech_002426 --> ing9_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing9_Biotin -.-> CHEBI_15956
    ing10_Folic_acid["Folic acid"]
    CultureMech_002426 --> ing10_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing10_Folic_acid -.-> CHEBI_27470
    ing11_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
    CultureMech_002426 --> ing11_Pyridoxine_hydrochloride
    CHEBI_30961["CHEBI:30961"]
    ing11_Pyridoxine_hydrochloride -.-> CHEBI_30961
    ing12_Thiamine_HCl["Thiamine HCl"]
    CultureMech_002426 --> ing12_Thiamine_HCl
    CHEBI_49105["CHEBI:49105"]
    ing12_Thiamine_HCl -.-> CHEBI_49105
    ing13_Riboflavin["Riboflavin"]
    CultureMech_002426 --> ing13_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing13_Riboflavin -.-> CHEBI_17015
    ing14_Nicotinic_acid["Nicotinic acid"]
    CultureMech_002426 --> ing14_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing14_Nicotinic_acid -.-> CHEBI_15940
    ing15_Calcium_pantothenate["Calcium pantothenate"]
    CultureMech_002426 --> ing15_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing15_Calcium_pantothenate -.-> CHEBI_31345
    ing16_Vitamin_B12["Vitamin B12"]
    CultureMech_002426 --> ing16_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing16_Vitamin_B12 -.-> CHEBI_176843
    ing17_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_002426 --> ing17_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing17_p_Aminobenzoic_acid -.-> CHEBI_30753
    ing18_Lipoic_acid["Lipoic acid"]
    CultureMech_002426 --> ing18_Lipoic_acid
    CHEBI_16494["CHEBI:16494"]
    ing18_Lipoic_acid -.-> CHEBI_16494

Ingredients (19)

# Ingredient Concentration Ontology Notes
1 KH2PO4
H2O4P.K 136.084 g/mol
0.390244 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
2 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
0.117073 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
3 NH4Cl
Cl.H4N 53.492 g/mol
0.097561 G_PER_L CHEBI:31206
ammonium chloride
4 K2SO4
2K.O4S 174.259 g/mol
0.00390244 G_PER_L CHEBI:32036
potassium sulfate
5 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.00292683 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
6 FeCl3 x 6 H2O
Cl3Fe.6H2O 270.294 g/mol
0.00487805 G_PER_L CHEBI:86254
iron trichloride hexahydrate
7 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.00487805 G_PER_L CHEBI:75213
sodium molybdate dihydrate
8 Sodium Thiosulfate
2Na.O3S2 158.111 g/mol
10 G_PER_L CHEBI:132112
sodium thiosulfate
9 NaHCO3
CHO3.Na 84.006 g/mol
5 G_PER_L CHEBI:32139
sodium hydrogencarbonate
10 Biotin
C10H16N2O3S 244.316 g/mol
0.002 G_PER_L CHEBI:15956
biotin
11 Folic acid
C19H19N7O6 441.404 g/mol
0.002 G_PER_L CHEBI:27470
folic acid
12 Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
0.01 G_PER_L CHEBI:30961
pyridoxine hydrochloride
13 Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
0.005 G_PER_L CHEBI:49105
thiamine hydrochloride
14 Riboflavin
C17H20N4O6 376.369 g/mol
0.005 G_PER_L CHEBI:17015
riboflavin
15 Nicotinic acid
C6H5NO2 123.111 g/mol
0.005 G_PER_L CHEBI:15940
nicotinic acid
16 Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.005 G_PER_L CHEBI:31345
Calcium pantothenate
17 Vitamin B12 0.0001 G_PER_L CHEBI:176843
vitamin B12
18 p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
0.005 G_PER_L CHEBI:30753
4-aminobenzoic acid
19 Lipoic acid
C8H14O2S2 206.332 g/mol
0.005 G_PER_L CHEBI:16494
lipoic acid

Preparation

  1. Mix components thoroughly and autoclave under a microaerophilic atmosphere (1.0 - 3.0% O2 in N2). Aseptically add per liter the following solutions (filter-sterilized):

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:19.211032Z mediadive-import Imported from MediaDive Source: JCM, ID: J1260
2026-03-13T06:19:13.231178Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002426
2026-03-17T06:29:53.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:26.321029+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "LSR1 MEDIUM" to "lsr1_medium" for consistent matching
2026-06-05T07:41:11.459319+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 19 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:00.593262+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.