Overview
- External ontology
- mediadive.medium:J1265 (source link unavailable) — GBS SALTS MEDIUM FOR THERMANAEROMONAS INYOENSIS
- Applications
-
- Microbial cultivation
- pH
- 7.0
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1265
Composition
flowchart LR
CultureMech_002431["gbs_salts_medium_for_thermanaeromonas_inyoensis"]
ing0_NaH2PO4["NaH2PO4"]
CultureMech_002431 --> ing0_NaH2PO4
CHEBI_37585["CHEBI:37585"]
ing0_NaH2PO4 -.-> CHEBI_37585
ing1_NaCl["NaCl"]
CultureMech_002431 --> ing1_NaCl
CHEBI_26710["CHEBI:26710"]
ing1_NaCl -.-> CHEBI_26710
ing2_NH4Cl["NH4Cl"]
CultureMech_002431 --> ing2_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing2_NH4Cl -.-> CHEBI_31206
ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_002431 --> ing3_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
ing4_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_002431 --> ing4_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing4_CaCl2_x_2_H2O -.-> CHEBI_86158
ing5_KCl["KCl"]
CultureMech_002431 --> ing5_KCl
CHEBI_32588["CHEBI:32588"]
ing5_KCl -.-> CHEBI_32588
ing6_Na2SO4["Na2SO4"]
CultureMech_002431 --> ing6_Na2SO4
CHEBI_32149["CHEBI:32149"]
ing6_Na2SO4 -.-> CHEBI_32149
ing7_Sodium_acetate["Sodium acetate"]
CultureMech_002431 --> ing7_Sodium_acetate
CHEBI_32954["CHEBI:32954"]
ing7_Sodium_acetate -.-> CHEBI_32954
ing8_Sodium_Thiosulfate["Sodium Thiosulfate"]
CultureMech_002431 --> ing8_Sodium_Thiosulfate
CHEBI_132112["CHEBI:132112"]
ing8_Sodium_Thiosulfate -.-> CHEBI_132112
ing9_Sodium_lactate["Sodium lactate"]
CultureMech_002431 --> ing9_Sodium_lactate
CHEBI_75228["CHEBI:75228"]
ing9_Sodium_lactate -.-> CHEBI_75228
ing10_NaHCO3["NaHCO3"]
CultureMech_002431 --> ing10_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing10_NaHCO3 -.-> CHEBI_32139
ing11_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_002431 --> ing11_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing11_Na2S_x_9_H2O -.-> CHEBI_76209
ing12_EDTA["EDTA"]
CultureMech_002431 --> ing12_EDTA
CHEBI_4735["CHEBI:4735"]
ing12_EDTA -.-> CHEBI_4735
ing13_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_002431 --> ing13_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing13_FeSO4_x_7_H2O -.-> CHEBI_75836
ing14_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_002431 --> ing14_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing14_MnCl2_x_4_H2O -.-> CHEBI_86368
ing15_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_002431 --> ing15_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing15_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing16_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_002431 --> ing16_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing16_CoCl2_x_6_H2O -.-> CHEBI_53503
ing17_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_002431 --> ing17_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing17_CuCl2_x_2_H2O -.-> CHEBI_86318
ing18_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_002431 --> ing18_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing18_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing19_H3BO3["H3BO3"]
CultureMech_002431 --> ing19_H3BO3
CHEBI_33118["CHEBI:33118"]
ing19_H3BO3 -.-> CHEBI_33118
ing20_Biotin["Biotin"]
CultureMech_002431 --> ing20_Biotin
CHEBI_15956["CHEBI:15956"]
ing20_Biotin -.-> CHEBI_15956
ing21_Folic_acid["Folic acid"]
CultureMech_002431 --> ing21_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing21_Folic_acid -.-> CHEBI_27470
ing22_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_002431 --> ing22_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing22_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing23_Thiamine_HCl["Thiamine HCl"]
CultureMech_002431 --> ing23_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing23_Thiamine_HCl -.-> CHEBI_49105
ing24_Riboflavin["Riboflavin"]
CultureMech_002431 --> ing24_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing24_Riboflavin -.-> CHEBI_17015
ing25_Nicotinic_acid["Nicotinic acid"]
CultureMech_002431 --> ing25_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing25_Nicotinic_acid -.-> CHEBI_15940
ing26_Calcium_pantothenate["Calcium pantothenate"]
CultureMech_002431 --> ing26_Calcium_pantothenate
CHEBI_31345["CHEBI:31345"]
ing26_Calcium_pantothenate -.-> CHEBI_31345
ing27_Vitamin_B12["Vitamin B12"]
CultureMech_002431 --> ing27_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing27_Vitamin_B12 -.-> CHEBI_176843
ing28_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_002431 --> ing28_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing28_p_Aminobenzoic_acid -.-> CHEBI_30753
ing29_Lipoic_acid["Lipoic acid"]
CultureMech_002431 --> ing29_Lipoic_acid
CHEBI_16494["CHEBI:16494"]
ing29_Lipoic_acid -.-> CHEBI_16494
Ingredients (30)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaH2PO4
H2O4P.Na 119.976 g/mol
|
1.34241 G_PER_L |
CHEBI:37585
sodium dihydrogenphosphate
|
|
| 2 |
NaCl
Cl.Na 58.443 g/mol
|
2.91829 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 3 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.104086 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 4 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.11965 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 5 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.0145914 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 6 |
KCl
Cl.K 74.551 g/mol
|
0.145914 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 7 |
Na2SO4
2Na.O4S 142.043 g/mol
|
0.291829 G_PER_L |
CHEBI:32149
sodium sulfate
|
|
| 8 |
Sodium acetate
C2H3O2.Na 82.034 g/mol
|
2 G_PER_L |
CHEBI:32954
sodium acetate
|
|
| 9 |
Sodium Thiosulfate
2Na.O3S2 158.111 g/mol
|
2 G_PER_L |
CHEBI:132112
sodium thiosulfate
|
|
| 10 |
Sodium lactate
C3H5O3.Na 112.06 g/mol
|
5 G_PER_L |
CHEBI:75228
sodium lactate
|
|
| 11 |
NaHCO3
CHO3.Na 84.006 g/mol
|
2 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 12 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
2 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
|
| 13 |
EDTA
C10H16N2O8 292.244 g/mol
|
3.7 G_PER_L |
CHEBI:4735
ethylenediaminetetraacetic acid
|
|
| 14 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
1.1 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 15 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.0984 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 16 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.0288 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 17 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.0238 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 18 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.0172 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 19 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.0242 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 20 |
H3BO3
H3BO3 61.833 g/mol
|
0.031 G_PER_L |
CHEBI:33118
boric acid
|
|
| 21 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.002 G_PER_L |
CHEBI:15956
biotin
|
|
| 22 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.002 G_PER_L |
CHEBI:27470
folic acid
|
|
| 23 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.01 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 24 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.005 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 25 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.005 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 26 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.005 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 27 |
Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.005 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 28 | Vitamin B12 | 0.0001 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 29 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.005 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 30 |
Lipoic acid
C8H14O2S2 206.332 g/mol
|
0.005 G_PER_L |
CHEBI:16494
lipoic acid
|
Preparation
- Mix components thoroughly, adjust pH to 7.0, distribute the medium into culture vessels (e.g., 5.0 ml in 25 ml serum bottles/Balch tubes) under a stream of N2, seal with butyl rubber stoppers and autoclave. After cooling, aseptically and anaerobically add per liter the following solutions (autoclaved or *filter-sterilized and stored anaerobically):
- Adjust pH to 6.0 with KOH.
- Comment: Consistent growth in serum tubes requires pretreatment of butyl rubber stoppers by boiling in 1% sodium sulfide solution (1 hr) followed by boiling in distilled H2O (1 hr, twice).
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:19.224414Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J1265 |
| 2026-03-13T06:19:13.262237Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002431 |
| 2026-03-17T06:30:53.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:26.357624+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "GBS SALTS MEDIUM FOR THERMANAEROMONAS INYOENSIS" to "gbs_salts_medium_for_thermanaeromonas_inyoensis" for consistent matching |
| 2026-06-05T07:41:05.981493+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 30 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:58.967495+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |