Overview
- External ontology
- mediadive.medium:J1274 (source link unavailable) — BM7CO MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.0
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1274
Composition
flowchart LR
CultureMech_002440["bm7co_medium"]
ing0_K2HPO4["K2HPO4"]
CultureMech_002440 --> ing0_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing0_K2HPO4 -.-> CHEBI_131527
ing1_KH2PO4["KH2PO4"]
CultureMech_002440 --> ing1_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing1_KH2PO4 -.-> CHEBI_63036
ing2_Urea["Urea"]
CultureMech_002440 --> ing2_Urea
CHEBI_16199["CHEBI:16199"]
ing2_Urea -.-> CHEBI_16199
ing3_Yeast_extract["Yeast extract"]
CultureMech_002440 --> ing3_Yeast_extract
ing4_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_002440 --> ing4_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing4_CaCl2_x_2_H2O -.-> CHEBI_86158
ing5_Na2CO3["Na2CO3"]
CultureMech_002440 --> ing5_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing5_Na2CO3 -.-> CHEBI_29377
ing6_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
CultureMech_002440 --> ing6_L_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing6_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
ing7_Resazurin["Resazurin"]
CultureMech_002440 --> ing7_Resazurin
CHEBI_8806["CHEBI:8806"]
ing7_Resazurin -.-> CHEBI_8806
ing8_Colloidal_chitin["Colloidal chitin"]
CultureMech_002440 --> ing8_Colloidal_chitin
ing9_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_002440 --> ing9_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing9_MgCl2_x_6_H2O -.-> CHEBI_86345
ing10_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_002440 --> ing10_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing10_FeSO4_x_7_H2O -.-> CHEBI_75836
Ingredients (11)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
K2HPO4
HO4P.2K 174.174 g/mol
|
2.9 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 2 |
KH2PO4
H2O4P.K 136.084 g/mol
|
1.5 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 3 |
Urea
CH4N2O 60.056 g/mol
|
2.1 G_PER_L |
CHEBI:16199
urea
|
|
| 4 | Yeast extract | 6 G_PER_L | ||
| 5 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
37.51 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
[Merged 2 duplicates: 0.01, 37.5] |
| 6 |
Na2CO3
CO3.2Na 105.988 g/mol
|
4 G_PER_L |
CHEBI:29377
sodium carbonate
|
|
| 7 |
L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.5 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
|
| 8 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.0005 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 9 | Colloidal chitin | 100 G_PER_L | ||
| 10 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
25 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 11 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.312 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
Preparation
- Mix components and adjust pH to 7.0. Distribute the medium in culture vessels (e.g., 9.0 ml in Balch tubes), replace the gas phase with N2 and seal with butyl rubber stoppers, and autoclave. After cooling, aseptically and anaerobically add per 900 ml the following solution (autoclaved under a N2 atmosphere):
- Colloidal chitin solution can be replaced by 10% (w/v) chitin or 0.2 M cellobiose solution.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:19.254239Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J1274 |
| 2026-03-13T03:08:52.649238Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:19:13.327843Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002440 |
| 2026-03-17T06:29:56.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:26.432540+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "BM7CO MEDIUM" to "bm7co_medium" for consistent matching |
| 2026-06-05T07:40:51.181836+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 9 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:53.929949+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |