methanol_utilizing_bacteria_medium_e

CultureMech:002446 bacterial DEFINED LIQUID
Original name: METHANOL-UTILIZING BACTERIA MEDIUM E

Overview

External ontology
mediadive.medium:J127 (source link unavailable) — METHANOL-UTILIZING BACTERIA MEDIUM E
Applications
  • Microbial cultivation
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=127

Composition

flowchart LR
    CultureMech_002446["methanol_utilizing_bacteria_medium_e"]
    ing0_NaCl["NaCl"]
    CultureMech_002446 --> ing0_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing0_NaCl -.-> CHEBI_26710
    ing1_Vitamin_B12["Vitamin B12"]
    CultureMech_002446 --> ing1_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing1_Vitamin_B12 -.-> CHEBI_176843
    ing2_Methanol["Methanol"]
    CultureMech_002446 --> ing2_Methanol
    CHEBI_17790["CHEBI:17790"]
    ing2_Methanol -.-> CHEBI_17790
    ing3__NH4_2SO4["(NH4)2SO4"]
    CultureMech_002446 --> ing3__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing3__NH4_2SO4 -.-> CHEBI_62946
    ing4_KH2PO4["KH2PO4"]
    CultureMech_002446 --> ing4_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing4_KH2PO4 -.-> CHEBI_63036
    ing5_Na2HPO4["Na2HPO4"]
    CultureMech_002446 --> ing5_Na2HPO4
    CHEBI_34683["CHEBI:34683"]
    ing5_Na2HPO4 -.-> CHEBI_34683
    ing6_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_002446 --> ing6_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing6_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing7_Ferric_citrate["Ferric citrate"]
    CultureMech_002446 --> ing7_Ferric_citrate
    CHEBI_144421["CHEBI:144421"]
    ing7_Ferric_citrate -.-> CHEBI_144421
    ing8_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_002446 --> ing8_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing8_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing9_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_002446 --> ing9_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing9_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing10_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_002446 --> ing10_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing10_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing11_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_002446 --> ing11_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing11_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing12_Thiamine_HCl["Thiamine HCl"]
    CultureMech_002446 --> ing12_Thiamine_HCl
    CHEBI_49105["CHEBI:49105"]
    ing12_Thiamine_HCl -.-> CHEBI_49105

Ingredients (13)

# Ingredient Concentration Ontology Notes
1 NaCl
Cl.Na 58.443 g/mol
30 G_PER_L CHEBI:26710
sodium chloride
2 Vitamin B12 10 G_PER_L CHEBI:176843
vitamin B12
3 Methanol
CH4O 32.042 g/mol
10 G_PER_L CHEBI:17790
methanol
4 (NH4)2SO4
2H4N.O4S 132.141 g/mol
2.9703 G_PER_L CHEBI:62946
ammonium sulfate
5 KH2PO4
H2O4P.K 136.084 g/mol
1.38614 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
6 Na2HPO4
HO4P.2Na 141.958 g/mol
2.9703 G_PER_L CHEBI:34683
disodium hydrogenphosphate
7 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.19802 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
8 Ferric citrate
C6H5FeO7 244.944 g/mol
0.029703 G_PER_L CHEBI:144421
iron(III) citrate
9 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.029703 G_PER_L CHEBI:86158
calcium chloride dihydrate
10 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.00495049 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
11 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.00495049 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
12 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
0.00049505 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
13 Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
0.00039604 G_PER_L CHEBI:49105
thiamine hydrochloride

Preparation

  1. Thiamine x HCl can be replaced by 0.2 g of yeast extract.
  2. Adjust pH to 7.1.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:15.774427Z mediadive-import Imported from MediaDive Source: JCM, ID: J127
2026-03-13T06:19:13.383251Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002446
2026-03-17T06:30:24.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:26.494253+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "METHANOL-UTILIZING BACTERIA MEDIUM E" to "methanol_utilizing_bacteria_medium_e" for consistent matching
2026-06-05T07:41:31.769280+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 13 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:07.072888+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.