Overview
- External ontology
- mediadive.medium:J1291 (source link unavailable) — GS2 MEDIUM WITH CELLOBIOSE
- Applications
-
- Microbial cultivation
- pH
- 7.2
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1291
Composition
flowchart LR
CultureMech_002453["gs2_medium_with_cellobiose"]
ing0_KH2PO4["KH2PO4"]
CultureMech_002453 --> ing0_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing0_KH2PO4 -.-> CHEBI_63036
ing1_K2HPO4["K2HPO4"]
CultureMech_002453 --> ing1_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing1_K2HPO4 -.-> CHEBI_131527
ing2_Urea["Urea"]
CultureMech_002453 --> ing2_Urea
CHEBI_16199["CHEBI:16199"]
ing2_Urea -.-> CHEBI_16199
ing3_MOPS["MOPS"]
CultureMech_002453 --> ing3_MOPS
CHEBI_39074["CHEBI:39074"]
ing3_MOPS -.-> CHEBI_39074
ing4_Yeast_extract["Yeast extract"]
CultureMech_002453 --> ing4_Yeast_extract
ing5_Trisodium_citrate_x_2_H2O["Trisodium citrate x 2 H2O"]
CultureMech_002453 --> ing5_Trisodium_citrate_x_2_H2O
CHEBI_53258["CHEBI:53258"]
ing5_Trisodium_citrate_x_2_H2O -.-> CHEBI_53258
ing6_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
CultureMech_002453 --> ing6_L_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing6_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
ing7_Resazurin["Resazurin"]
CultureMech_002453 --> ing7_Resazurin
CHEBI_8806["CHEBI:8806"]
ing7_Resazurin -.-> CHEBI_8806
ing8_Cellobiose["Cellobiose"]
CultureMech_002453 --> ing8_Cellobiose
CHEBI_17057["CHEBI:17057"]
ing8_Cellobiose -.-> CHEBI_17057
ing9_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_002453 --> ing9_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing9_MgCl2_x_6_H2O -.-> CHEBI_86345
ing10_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_002453 --> ing10_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing10_CaCl2_x_2_H2O -.-> CHEBI_86158
ing11_FeSO4["FeSO4"]
CultureMech_002453 --> ing11_FeSO4
CHEBI_75832["CHEBI:75832"]
ing11_FeSO4 -.-> CHEBI_75832
Ingredients (12)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
KH2PO4
H2O4P.K 136.084 g/mol
|
1.47348 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 2 |
K2HPO4
HO4P.2K 174.174 g/mol
|
2.84872 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 3 |
Urea
CH4N2O 60.056 g/mol
|
2.06287 G_PER_L |
CHEBI:16199
urea
|
|
| 4 | MOPS | 9.82318 G_PER_L |
CHEBI:39074
MOPS
|
|
| 5 | Yeast extract | 5.89391 G_PER_L | ||
| 6 |
Trisodium citrate x 2 H2O
C6H5O7.3Na 258.069 g/mol
|
2.84872 G_PER_L |
CHEBI:53258
sodium citrate
|
|
| 7 |
L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.24558 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
|
| 8 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.000982318 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 9 |
Cellobiose
C12H22O11 342.297 g/mol
|
8 G_PER_L |
CHEBI:17057
cellobiose
|
|
| 10 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
100 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 11 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
15 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 12 |
FeSO4
Fe.O4S 151.908 g/mol
|
0.1 G_PER_L |
CHEBI:75832
iron(2+) sulfate (anhydrous)
|
Preparation
- Mix components thoroughly and adjust pH to 7.2 with NaOH. Bring to a boil, then cool down and distribute into culture vessels under a N2 gas stream, seal with butyl rubber stoppers, and autoclave. After cooling, aseptically and anaerobically add the following solution (autoclaved):
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:19.327114Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J1291 |
| 2026-01-28T19:48:27.800859Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:19:13.442185Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002453 |
| 2026-03-17T06:30:24.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:26.559564+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "GS2 MEDIUM WITH CELLOBIOSE" to "gs2_medium_with_cellobiose" for consistent matching |
| 2026-06-05T07:41:06.740250+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 11 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:59.192643+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |