mmj_thi_medium

CultureMech:002459 bacterial DEFINED LIQUID
Original name: MMJ/THI MEDIUM

Overview

External ontology
mediadive.medium:J1297 (source link unavailable) — MMJ/THI MEDIUM
Applications
  • Microbial cultivation
pH
7.0
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1297

Composition

flowchart LR
    CultureMech_002459["mmj_thi_medium"]
    ing0_NaCl["NaCl"]
    CultureMech_002459 --> ing0_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing0_NaCl -.-> CHEBI_26710
    ing1_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_002459 --> ing1_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing1_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_002459 --> ing2_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing3_KCl["KCl"]
    CultureMech_002459 --> ing3_KCl
    CHEBI_32588["CHEBI:32588"]
    ing3_KCl -.-> CHEBI_32588
    ing4_NH4Cl["NH4Cl"]
    CultureMech_002459 --> ing4_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing4_NH4Cl -.-> CHEBI_31206
    ing5_K2HPO4["K2HPO4"]
    CultureMech_002459 --> ing5_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing5_K2HPO4 -.-> CHEBI_131527
    ing6_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_002459 --> ing6_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing6_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing7_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_002459 --> ing7_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing7_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing8_Na2SeO3["Na2SeO3"]
    CultureMech_002459 --> ing8_Na2SeO3
    CHEBI_48843["CHEBI:48843"]
    ing8_Na2SeO3 -.-> CHEBI_48843
    ing9_Fe_NH4_2_SO4_2_x_6_H2O["Fe(NH4)2(SO4)2 x 6 H2O"]
    CultureMech_002459 --> ing9_Fe_NH4_2_SO4_2_x_6_H2O
    CHEBI_76181["CHEBI:76181"]
    ing9_Fe_NH4_2_SO4_2_x_6_H2O -.-> CHEBI_76181
    ing10_PIPES["PIPES"]
    CultureMech_002459 --> ing10_PIPES
    CHEBI_39033["CHEBI:39033"]
    ing10_PIPES -.-> CHEBI_39033
    ing11_Na2S2O3_x_5_H2O["Na2S2O3 x 5 H2O"]
    CultureMech_002459 --> ing11_Na2S2O3_x_5_H2O
    CHEBI_32150["CHEBI:32150"]
    ing11_Na2S2O3_x_5_H2O -.-> CHEBI_32150
    ing12_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
    CultureMech_002459 --> ing12_Nitrilotriacetic_acid
    CHEBI_44557["CHEBI:44557"]
    ing12_Nitrilotriacetic_acid -.-> CHEBI_44557
    ing13_MnSO4_x_n_H2O["MnSO4 x n H2O"]
    CultureMech_002459 --> ing13_MnSO4_x_n_H2O
    CHEBI_86360["CHEBI:86360"]
    ing13_MnSO4_x_n_H2O -.-> CHEBI_86360
    ing14_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_002459 --> ing14_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing14_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing15_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
    CultureMech_002459 --> ing15_CoSO4_x_7_H2O
    CHEBI_91244["CHEBI:91244"]
    ing15_CoSO4_x_7_H2O -.-> CHEBI_91244
    ing16_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_002459 --> ing16_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing16_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing17_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_002459 --> ing17_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing17_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing18_AlK_SO4_2["AlK(SO4)2"]
    CultureMech_002459 --> ing18_AlK_SO4_2
    CHEBI_86463["CHEBI:86463"]
    ing18_AlK_SO4_2 -.-> CHEBI_86463
    ing19_H3BO3["H3BO3"]
    CultureMech_002459 --> ing19_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing19_H3BO3 -.-> CHEBI_33118
    ing20_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_002459 --> ing20_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing20_Na2MoO4_x_2_H2O -.-> CHEBI_75213

Ingredients (21)

# Ingredient Concentration Ontology Notes
1 NaCl
Cl.Na 58.443 g/mol
30.6736 G_PER_L CHEBI:26710
sodium chloride
 [Merged 2 duplicates: 29.6736, 1.0]
2 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
4.1543 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
3 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
6.36301 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
 [Merged 2 duplicates: 3.36301, 3.0]
4 KCl
Cl.K 74.551 g/mol
0.326409 G_PER_L CHEBI:32588
potassium chloride
5 NH4Cl
Cl.H4N 53.492 g/mol
0.24728 G_PER_L CHEBI:31206
ammonium chloride
6 K2HPO4
HO4P.2K 174.174 g/mol
0.138477 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
7 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.238477 G_PER_L CHEBI:86158
calcium chloride dihydrate
 [Merged 2 duplicates: 0.138477, 0.1]
8 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.00049456 G_PER_L CHEBI:34887
nickel dichloride
9 Na2SeO3
2Na.O3Se 172.937 g/mol
0.000296736 G_PER_L CHEBI:48843
disodium selenite
10 Fe(NH4)2(SO4)2 x 6 H2O
Fe.6H2O.2H4N.2O4S 392.139 g/mol
0.0098912 G_PER_L CHEBI:76181
ferrous ammonium sulfate hexahydrate
11 PIPES 5.93472 G_PER_L CHEBI:39033
PIPES
12 Na2S2O3 x 5 H2O
5H2O.2Na.O3S2 248.186 g/mol
4.9456 G_PER_L CHEBI:32150
sodium thiosulfate pentahydrate
13 Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
1.5 G_PER_L CHEBI:44557
nitrilotriacetic acid
14 MnSO4 x n H2O
Mn.O4S 151.001 g/mol
0.5 G_PER_L CHEBI:86360
manganese(II) sulfate
15 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.1 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
16 CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
0.1 G_PER_L CHEBI:91244
cobalt(2+) sulfate heptahydrate
17 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.1 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
18 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
0.01 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
19 AlK(SO4)2
Al.K.2O4S 258.206 g/mol
0.01 G_PER_L CHEBI:86463
potassium aluminium sulfate
20 H3BO3
H3BO3 61.833 g/mol
0.01 G_PER_L CHEBI:33118
boric acid
21 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.01 G_PER_L CHEBI:75213
sodium molybdate dihydrate

Preparation

  1. Adjust pH to 7.0.
  2. Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0.

Solutions

Vitamin solution

Dose: 1 G_PER_L

See mediadive_6241_Vitamin_solution.yaml for composition

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:19.348672Z mediadive-import Imported from MediaDive Source: JCM, ID: J1297
2026-01-28T19:48:09.542309Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-09T08:04:15.440758+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 1 solution(s) from ingredients
2026-03-13T03:08:42.656940Z data-quality-cleanup-v1.0 Data quality fixes Merged 3 duplicate ingredient(s)
2026-03-13T06:19:13.498339Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002459
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name
2026-03-17T06:29:45.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:26.621452+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "MMJ/THI MEDIUM" to "mmj_thi_medium" for consistent matching
2026-05-16T09:04:24.572042+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1
2026-06-05T07:41:35.531387+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 20 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:08.298409+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.