gam_broth_with_sodium_pyruvate

CultureMech:002478 bacterial COMPLEX LIQUID
Original name: GAM BROTH WITH SODIUM PYRUVATE

Overview

External ontology
mediadive.medium:J1314 (source link unavailable) — GAM BROTH WITH SODIUM PYRUVATE
Applications
  • Microbial cultivation
pH
7.0
Notes
JCM Medium 1314 lists 59.0 g GAM broth from Nissui, 10.0 g sodium pyruvate, and 1.0 L distilled water; it adjusts pH to 7.0 and uses JCM's default autoclaving at 121 C for 15 min.

Composition

flowchart LR
    CultureMech_002478["gam_broth_with_sodium_pyruvate"]
    ing0_GAM_broth__Nissui_["GAM broth (Nissui)"]
    CultureMech_002478 --> ing0_GAM_broth__Nissui_
    ing1_Sodium_pyruvate["Sodium pyruvate"]
    CultureMech_002478 --> ing1_Sodium_pyruvate
    CHEBI_50144["CHEBI:50144"]
    ing1_Sodium_pyruvate -.-> CHEBI_50144
    ing2_Distilled_water["Distilled water"]
    CultureMech_002478 --> ing2_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing2_Distilled_water -.-> CHEBI_15377

Ingredients (3)

# Ingredient Concentration Ontology Notes
1 GAM broth (Nissui) 59.0 G_PER_L JCM Medium 1314 lists 59.0 g/L GAM broth from Nissui without disclosing the product composition.
2 Sodium pyruvate
C3H3O3.Na 110.044 g/mol
10.0 G_PER_L CHEBI:50144
sodium pyruvate
 JCM Medium 1314 lists 10.0 g/L sodium pyruvate.
3 Distilled water
H2O 18.015 g/mol
1000.0 ML_PER_L CHEBI:15377
water
 JCM Medium 1314 lists 1.0 L distilled water.

Preparation

  1. Mix 59.0 g GAM broth from Nissui and 10.0 g sodium pyruvate with 1.0 L distilled water.
  2. Adjust pH to 7.0.
  3. Sterilize by autoclaving at 121 C for 15 min.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:19.413231Z mediadive-import Imported from MediaDive Source: JCM, ID: J1314
2026-03-13T06:19:13.665393Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002478
2026-03-17T06:29:52.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:26.811039+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "GAM BROTH WITH SODIUM PYRUVATE" to "gam_broth_with_sodium_pyruvate" for consistent matching
2026-06-05T07:41:05.939461+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 1 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-09-12T00:00:00-07:00 repair_jcm_1314_gam_pyruvate_score15.py RESOLVED_JCM_1314_GAM_PYRUVATE_SCORE15 JCM Medium 1314 lists 59.0 g GAM broth from Nissui, 10.0 g sodium pyruvate, and 1.0 L distilled water; it adjusts pH to 7.0 and uses JCM's default autoclaving at 121 C for 15 min.