rhodopseudomonas_medium

CultureMech:002483 bacterial COMPLEX LIQUID
Original name: RHODOPSEUDOMONAS MEDIUM

Overview

External ontology
mediadive.medium:J131 (source link unavailable) — RHODOPSEUDOMONAS MEDIUM
Applications
  • Microbial cultivation
pH
7.0
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=131

Composition

flowchart LR
    CultureMech_002483["rhodopseudomonas_medium"]
    ing0_Sodium_succinate["Sodium succinate"]
    CultureMech_002483 --> ing0_Sodium_succinate
    CHEBI_63675["CHEBI:63675"]
    ing0_Sodium_succinate -.-> CHEBI_63675
    ing1_K2HPO4["K2HPO4"]
    CultureMech_002483 --> ing1_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing1_K2HPO4 -.-> CHEBI_131527
    ing2_KH2PO4["KH2PO4"]
    CultureMech_002483 --> ing2_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing2_KH2PO4 -.-> CHEBI_63036
    ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_002483 --> ing3_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing4__NH4_2SO4["(NH4)2SO4"]
    CultureMech_002483 --> ing4__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing4__NH4_2SO4 -.-> CHEBI_62946
    ing5_CaCl2["CaCl2"]
    CultureMech_002483 --> ing5_CaCl2
    CHEBI_3312["CHEBI:3312"]
    ing5_CaCl2 -.-> CHEBI_3312
    ing6_Ferric_citrate["Ferric citrate"]
    CultureMech_002483 --> ing6_Ferric_citrate
    CHEBI_144421["CHEBI:144421"]
    ing6_Ferric_citrate -.-> CHEBI_144421
    ing7_EDTA["EDTA"]
    CultureMech_002483 --> ing7_EDTA
    CHEBI_4735["CHEBI:4735"]
    ing7_EDTA -.-> CHEBI_4735
    ing8_Yeast_extract["Yeast extract"]
    CultureMech_002483 --> ing8_Yeast_extract

Ingredients (9)

# Ingredient Concentration Ontology Notes
1 Sodium succinate
C4H4O4.2Na 162.052 g/mol
2.5 G_PER_L CHEBI:63675
sodium succinate (anhydrous)
2 K2HPO4
HO4P.2K 174.174 g/mol
0.9 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
3 KH2PO4
H2O4P.K 136.084 g/mol
0.6 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
4 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.2 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
5 (NH4)2SO4
2H4N.O4S 132.141 g/mol
1.25 G_PER_L CHEBI:62946
ammonium sulfate
6 CaCl2
Ca.2Cl 110.984 g/mol
0.07 G_PER_L CHEBI:3312
calcium dichloride
7 Ferric citrate
C6H5FeO7 244.944 g/mol
0.003 G_PER_L CHEBI:144421
iron(III) citrate
8 EDTA
C10H16N2O8 292.244 g/mol
0.003 G_PER_L CHEBI:4735
ethylenediaminetetraacetic acid
9 Yeast extract 0.5 G_PER_L

Preparation

  1. Adjust pH to 7.0.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:15.783270Z mediadive-import Imported from MediaDive Source: JCM, ID: J131
2026-03-13T06:19:13.712189Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002483
2026-03-17T06:30:41.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:26.858900+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "RHODOPSEUDOMONAS MEDIUM" to "rhodopseudomonas_medium" for consistent matching
2026-06-05T07:41:44.389802+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 8 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:10.982275+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-10-07T14:33:27.670564+00:00 codex CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged.