jcm_medium_no_1324

CultureMech:002488 bacterial DEFINED LIQUID
Original name: JCM MEDIUM No. 1324

Overview

External ontology
mediadive.medium:J1324 (source link unavailable) — JCM MEDIUM No. 1324
Applications
  • Microbial cultivation
pH
6.8
Notes
JCM Medium 1324 was repaired from its imported Main sol. 1324 fragment: defined salts in 1 L distilled water, 0.5% sterile methanol added after sterilization, and pH 6.8.

Composition

flowchart LR
    CultureMech_002488["jcm_medium_no_1324"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_002488 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_NH4Cl["NH4Cl"]
    CultureMech_002488 --> ing1_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing1_NH4Cl -.-> CHEBI_31206
    ing2_K2HPO4["K2HPO4"]
    CultureMech_002488 --> ing2_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing2_K2HPO4 -.-> CHEBI_131527
    ing3_KH2PO4["KH2PO4"]
    CultureMech_002488 --> ing3_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing3_KH2PO4 -.-> CHEBI_63036
    ing4_MgSO4_x_6_H2O["MgSO4 x 6 H2O"]
    CultureMech_002488 --> ing4_MgSO4_x_6_H2O
    CHEBI_32599["CHEBI:32599"]
    ing4_MgSO4_x_6_H2O -.-> CHEBI_32599
    ing5_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_002488 --> ing5_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing5_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing6_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_002488 --> ing6_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing6_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing7_Methanol["Methanol"]
    CultureMech_002488 --> ing7_Methanol
    CHEBI_17790["CHEBI:17790"]
    ing7_Methanol -.-> CHEBI_17790

Ingredients (8)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
1.0 L CHEBI:15377
water
 JCM Medium 1324 / Main sol. 1324 lists 1.0 L Distilled water.
2 NH4Cl
Cl.H4N 53.492 g/mol
0.5 G_PER_L CHEBI:31206
ammonium chloride
 JCM Medium 1324 / Main sol. 1324 lists 0.5 g/L NH4Cl.
3 K2HPO4
HO4P.2K 174.174 g/mol
0.7 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
 JCM Medium 1324 / Main sol. 1324 lists 0.7 g/L K2HPO4.
4 KH2PO4
H2O4P.K 136.084 g/mol
0.54 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
 JCM Medium 1324 / Main sol. 1324 lists 0.54 g/L KH2PO4.
5 MgSO4 x 6 H2O
Mg.O4S 120.368 g/mol
1 G_PER_L CHEBI:32599
magnesium sulfate
 JCM Medium 1324 / Main sol. 1324 lists 1 g/L MgSO4 x 6 H2O.
6 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.2 G_PER_L CHEBI:86158
calcium chloride dihydrate
 JCM Medium 1324 / Main sol. 1324 lists 0.2 g/L CaCl2 x 2 H2O.
7 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.004 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
 JCM Medium 1324 / Main sol. 1324 lists 0.004 g/L FeSO4 x 7 H2O.
8 Methanol
CH4O 32.042 g/mol
5 ML_PER_L CHEBI:17790
methanol
 JCM Medium 1324 / Main sol. 1324 adds sterile methanol to a 0.5% final concentration in the sterile medium.

Preparation

  1. Mix the salts in distilled water for the main solution; withhold methanol until after sterilization.
  2. Adjust pH to 6.8.
  3. Sterilize the main solution.
  4. Add sterile methanol to a final concentration of 0.5% in the sterile medium.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:19.450245Z mediadive-import Imported from MediaDive Source: JCM, ID: J1324
2026-02-03T11:26:53.895074 quality-tagger-v1.0 Added data quality flag Marked as incomplete_composition due to placeholder ingredients
2026-03-13T06:19:13.747401Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002488
2026-03-17T19:45:26.902495+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "JCM MEDIUM No. 1324" to "jcm_medium_no_1324" for consistent matching
2026-04-05T17:20:25.512459 mark_unavailable_media MARKED_UNAVAILABLE Source database has no composition data. Category: EMPTY. JCM page returns "Nothing found"
2026-05-16T08:57:36.152483+00:00 migrate_data_quality_flags.py MIGRATED_DATA_QUALITY_FLAGS dict(2 keys) -> list(2 entries)
2026-08-06T07:56:36.102653+00:00 curate_placeholder_composition.py REMOVED_PLACEHOLDER_INGREDIENT Removed the 'See source for composition' placeholder ingredient; the record carries no imported composition and is flagged incomplete (#175).
2026-09-10T00:00:00-07:00 repair_jcm_1324_score35.py RESOLVED_JCM_1324_SCORE35 JCM Medium 1324 was repaired from its imported Main sol. 1324 fragment: defined salts in 1 L distilled water, 0.5% sterile methanol added after sterilization, and pH 6.8.