medium_10

CultureMech:002493 bacterial COMPLEX SOLID_AGAR
Original name: MEDIUM 10

Overview

External ontology
mediadive.medium:J133 (source link unavailable) — MEDIUM 10
Applications
  • Microbial cultivation
pH
6.8
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=133

Composition

flowchart LR
    CultureMech_002493["medium_10"]
    ing0_Trypticase_peptone["Trypticase peptone"]
    CultureMech_002493 --> ing0_Trypticase_peptone
    ing1_Yeast_extract["Yeast extract"]
    CultureMech_002493 --> ing1_Yeast_extract
    ing2_Hemin["Hemin"]
    CultureMech_002493 --> ing2_Hemin
    CHEBI_50385["CHEBI:50385"]
    ing2_Hemin -.-> CHEBI_50385
    ing3_Glucose["Glucose"]
    CultureMech_002493 --> ing3_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing3_Glucose -.-> CHEBI_17234
    ing4_Cellobiose["Cellobiose"]
    CultureMech_002493 --> ing4_Cellobiose
    CHEBI_17057["CHEBI:17057"]
    ing4_Cellobiose -.-> CHEBI_17057
    ing5_Starch["Starch"]
    CultureMech_002493 --> ing5_Starch
    CHEBI_28017["CHEBI:28017"]
    ing5_Starch -.-> CHEBI_28017
    ing6_Agar["Agar"]
    CultureMech_002493 --> ing6_Agar
    CHEBI_2509["CHEBI:2509"]
    ing6_Agar -.-> CHEBI_2509
    ing7_Resazurin["Resazurin"]
    CultureMech_002493 --> ing7_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing7_Resazurin -.-> CHEBI_8806
    ing8_Toray_silicone_SH_5535["Toray silicone SH 5535"]
    CultureMech_002493 --> ing8_Toray_silicone_SH_5535
    ing9_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
    CultureMech_002493 --> ing9_L_Cysteine_HCl_x_H2O
    CHEBI_91248["CHEBI:91248"]
    ing9_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
    ing10_L_Ascorbic_acid["L-Ascorbic acid"]
    CultureMech_002493 --> ing10_L_Ascorbic_acid
    CHEBI_29073["CHEBI:29073"]
    ing10_L_Ascorbic_acid -.-> CHEBI_29073
    ing11_Na2SO3["Na2SO3"]
    CultureMech_002493 --> ing11_Na2SO3
    CHEBI_86477["CHEBI:86477"]
    ing11_Na2SO3 -.-> CHEBI_86477
    ing12_Acetic_acid["Acetic acid"]
    CultureMech_002493 --> ing12_Acetic_acid
    CHEBI_15366["CHEBI:15366"]
    ing12_Acetic_acid -.-> CHEBI_15366
    ing13_Propionic_acid["Propionic acid"]
    CultureMech_002493 --> ing13_Propionic_acid
    CHEBI_30768["CHEBI:30768"]
    ing13_Propionic_acid -.-> CHEBI_30768
    ing14_n_Butyric_acid["n-Butyric acid"]
    CultureMech_002493 --> ing14_n_Butyric_acid
    CHEBI_30772["CHEBI:30772"]
    ing14_n_Butyric_acid -.-> CHEBI_30772
    ing15_iso_Butyric_acid["iso-Butyric acid"]
    CultureMech_002493 --> ing15_iso_Butyric_acid
    CHEBI_16135["CHEBI:16135"]
    ing15_iso_Butyric_acid -.-> CHEBI_16135
    ing16_n_Valeric_acid["n-Valeric acid"]
    CultureMech_002493 --> ing16_n_Valeric_acid
    CHEBI_17418["CHEBI:17418"]
    ing16_n_Valeric_acid -.-> CHEBI_17418
    ing17_iso_Valeric_acid["iso-Valeric acid"]
    CultureMech_002493 --> ing17_iso_Valeric_acid
    CHEBI_28484["CHEBI:28484"]
    ing17_iso_Valeric_acid -.-> CHEBI_28484
    ing18_DL_alpha_Methyl_butyric_acid["DL-alpha-Methyl butyric acid"]
    CultureMech_002493 --> ing18_DL_alpha_Methyl_butyric_acid
    CHEBI_37070["CHEBI:37070"]
    ing18_DL_alpha_Methyl_butyric_acid -.-> CHEBI_37070
    ing19_K2HPO4["K2HPO4"]
    CultureMech_002493 --> ing19_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing19_K2HPO4 -.-> CHEBI_131527
    ing20_KH2PO4["KH2PO4"]
    CultureMech_002493 --> ing20_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing20_KH2PO4 -.-> CHEBI_63036
    ing21_NaCl["NaCl"]
    CultureMech_002493 --> ing21_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing21_NaCl -.-> CHEBI_26710
    ing22__NH4_2SO4["(NH4)2SO4"]
    CultureMech_002493 --> ing22__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing22__NH4_2SO4 -.-> CHEBI_62946
    ing23_CaCl2["CaCl2"]
    CultureMech_002493 --> ing23_CaCl2
    CHEBI_3312["CHEBI:3312"]
    ing23_CaCl2 -.-> CHEBI_3312
    ing24_MgSO4_x_H2O["MgSO4 x H2O"]
    CultureMech_002493 --> ing24_MgSO4_x_H2O
    CHEBI_32599["CHEBI:32599"]
    ing24_MgSO4_x_H2O -.-> CHEBI_32599

Ingredients (25)

# Ingredient Concentration Ontology Notes
1 Trypticase peptone 1.91022 G_PER_L
2 Yeast extract 0.477555 G_PER_L
3 Hemin
C34H32ClFeN4O4 651.952 g/mol
1 G_PER_L CHEBI:50385
hemin
4 Glucose
C6H12O6 180.156 g/mol
0.477555 G_PER_L CHEBI:17234
glucose
5 Cellobiose
C12H22O11 342.297 g/mol
0.477555 G_PER_L CHEBI:17057
cellobiose
6 Starch 0.477555 G_PER_L CHEBI:28017
starch
7 Agar 17.192 G_PER_L CHEBI:2509
agar
8 Resazurin
C12H7NO4 229.191 g/mol
0.5 G_PER_L CHEBI:8806
Resazurin
9 Toray silicone SH 5535 5 G_PER_L
10 L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
10 G_PER_L CHEBI:91248
L-cysteine hydrochloride hydrate
11 L-Ascorbic acid
C6H8O6 176.124 g/mol
2 G_PER_L CHEBI:29073
L-ascorbic acid
12 Na2SO3
2Na.O3S 126.044 g/mol
100 G_PER_L CHEBI:86477
sodium sulfite
13 Acetic acid
C2H4O2 60.052 g/mol
17 G_PER_L CHEBI:15366
acetic acid
14 Propionic acid
C3H6O2 74.079 g/mol
6 G_PER_L CHEBI:30768
propionic acid
15 n-Butyric acid
C4H8O2 88.106 g/mol
4 G_PER_L CHEBI:30772
butyric acid
16 iso-Butyric acid
C4H8O2 88.106 g/mol
1 G_PER_L CHEBI:16135
isobutyric acid
17 n-Valeric acid
C5H10O2 102.133 g/mol
1 G_PER_L CHEBI:17418
valeric acid
18 iso-Valeric acid
C5H10O2 102.133 g/mol
1 G_PER_L CHEBI:28484
isovaleric acid
19 DL-alpha-Methyl butyric acid
C5H10O2 102.133 g/mol
1 G_PER_L CHEBI:37070
2-methylbutyric acid
20 K2HPO4
HO4P.2K 174.174 g/mol
7.8 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
21 KH2PO4
H2O4P.K 136.084 g/mol
4.7 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
22 NaCl
Cl.Na 58.443 g/mol
11.8 G_PER_L CHEBI:26710
sodium chloride
23 (NH4)2SO4
2H4N.O4S 132.141 g/mol
12 G_PER_L CHEBI:62946
ammonium sulfate
24 CaCl2
Ca.2Cl 110.984 g/mol
1.2 G_PER_L CHEBI:3312
calcium dichloride
25 MgSO4 x H2O
Mg.O4S 120.368 g/mol
2.5 G_PER_L CHEBI:32599
magnesium sulfate

Preparation

  1. Adjust pH to 6.7-6.8.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-06-14T05:58:58.561437+00:00 idnf-grounding-fix-v1.0 Fixed ID_NOT_FOUND term groundings Absent/obsolete CHEBI term ids fixed - 1 re-grounded to the correct CHEBI, 0 de-grounded (mixture or unverifiable).
2026-01-26T17:53:15.785618Z mediadive-import Imported from MediaDive Source: JCM, ID: J133
2026-03-13T06:19:13.795639Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002493
2026-03-17T06:30:47.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:26.957050+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "MEDIUM 10" to "medium_10" for consistent matching
2026-06-05T07:41:13.539361+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 21 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:01.099764+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.