Overview
- External ontology
- mediadive.medium:J134 (source link unavailable) — RUMEN FLUID-GLUCOSE-CELLOBIOSE AGAR
- Applications
-
- Microbial cultivation
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=134
Composition
flowchart LR
CultureMech_002494["rumen_fluid_glucose_cellobiose_agar"]
ing0_Glucose["Glucose"]
CultureMech_002494 --> ing0_Glucose
CHEBI_17234["CHEBI:17234"]
ing0_Glucose -.-> CHEBI_17234
ing1_Cellobiose["Cellobiose"]
CultureMech_002494 --> ing1_Cellobiose
CHEBI_17057["CHEBI:17057"]
ing1_Cellobiose -.-> CHEBI_17057
ing2_Starch["Starch"]
CultureMech_002494 --> ing2_Starch
CHEBI_28017["CHEBI:28017"]
ing2_Starch -.-> CHEBI_28017
ing3__NH4_2SO4["(NH4)2SO4"]
CultureMech_002494 --> ing3__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing3__NH4_2SO4 -.-> CHEBI_62946
ing4_Resazurin["Resazurin"]
CultureMech_002494 --> ing4_Resazurin
CHEBI_8806["CHEBI:8806"]
ing4_Resazurin -.-> CHEBI_8806
ing5_Rumen_fluid["Rumen fluid"]
CultureMech_002494 --> ing5_Rumen_fluid
ing6_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
CultureMech_002494 --> ing6_L_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing6_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
ing7_Hemin["Hemin"]
CultureMech_002494 --> ing7_Hemin
CHEBI_50385["CHEBI:50385"]
ing7_Hemin -.-> CHEBI_50385
ing8_Vitamin_K1["Vitamin K1"]
CultureMech_002494 --> ing8_Vitamin_K1
CHEBI_18067["CHEBI:18067"]
ing8_Vitamin_K1 -.-> CHEBI_18067
ing9_Agar["Agar"]
CultureMech_002494 --> ing9_Agar
CHEBI_2509["CHEBI:2509"]
ing9_Agar -.-> CHEBI_2509
ing10_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_002494 --> ing10_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing10_CaCl2_x_2_H2O -.-> CHEBI_86158
ing11_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_002494 --> ing11_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing11_MgSO4_x_7_H2O -.-> CHEBI_31795
ing12_K2HPO4["K2HPO4"]
CultureMech_002494 --> ing12_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing12_K2HPO4 -.-> CHEBI_131527
ing13_KH2PO4["KH2PO4"]
CultureMech_002494 --> ing13_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing13_KH2PO4 -.-> CHEBI_63036
ing14_NaHCO3["NaHCO3"]
CultureMech_002494 --> ing14_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing14_NaHCO3 -.-> CHEBI_32139
ing15_NaCl["NaCl"]
CultureMech_002494 --> ing15_NaCl
CHEBI_26710["CHEBI:26710"]
ing15_NaCl -.-> CHEBI_26710
Ingredients (16)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Glucose
C6H12O6 180.156 g/mol
|
0.244576 G_PER_L |
CHEBI:17234
glucose
|
|
| 2 |
Cellobiose
C12H22O11 342.297 g/mol
|
0.244576 G_PER_L |
CHEBI:17057
cellobiose
|
|
| 3 | Starch | 0.493097 G_PER_L |
CHEBI:28017
starch
|
|
| 4 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
0.986193 G_PER_L |
CHEBI:62946
ammonium sulfate
|
|
| 5 |
Resazurin
C12H7NO4 229.191 g/mol
|
4 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 6 | Rumen fluid | 300 G_PER_L | ||
| 7 |
L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.493097 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
|
| 8 |
Hemin
C34H32ClFeN4O4 651.952 g/mol
|
10 G_PER_L |
CHEBI:50385
hemin
|
|
| 9 |
Vitamin K1
C31H46O2 450.707 g/mol
|
0.2 G_PER_L |
CHEBI:18067
phylloquinone
|
|
| 10 | Agar | 19.7239 G_PER_L |
CHEBI:2509
agar
|
|
| 11 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.2 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 12 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.2 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 13 |
K2HPO4
HO4P.2K 174.174 g/mol
|
1 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 14 |
KH2PO4
H2O4P.K 136.084 g/mol
|
1 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 15 |
NaHCO3
CHO3.Na 84.006 g/mol
|
10 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 16 |
NaCl
Cl.Na 58.443 g/mol
|
2 G_PER_L |
CHEBI:26710
sodium chloride
|
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:15.789620Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J134 |
| 2026-03-13T06:19:13.803770Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002494 |
| 2026-03-17T06:30:38.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:26.966583+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "RUMEN FLUID-GLUCOSE-CELLOBIOSE AGAR" to "rumen_fluid_glucose_cellobiose_agar" for consistent matching |
| 2026-06-05T07:41:44.793561+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 15 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:11.127535+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |