Overview
- External ontology
- mediadive.medium:J143 (source link unavailable) — LEUCONOSTOC OENOS MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 4.8
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=143
Composition
flowchart LR
CultureMech_002502["leuconostoc_oenos_medium"]
ing0_Casein_peptone["Casein peptone"]
CultureMech_002502 --> ing0_Casein_peptone
ing1_Yeast_extract["Yeast extract"]
CultureMech_002502 --> ing1_Yeast_extract
ing2_Glucose["Glucose"]
CultureMech_002502 --> ing2_Glucose
CHEBI_17234["CHEBI:17234"]
ing2_Glucose -.-> CHEBI_17234
ing3_Fructose["Fructose"]
CultureMech_002502 --> ing3_Fructose
CHEBI_28757["CHEBI:28757"]
ing3_Fructose -.-> CHEBI_28757
ing4_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_002502 --> ing4_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing4_MgSO4_x_7_H2O -.-> CHEBI_31795
ing5_MnSO4_x_n_H2O["MnSO4 x n H2O"]
CultureMech_002502 --> ing5_MnSO4_x_n_H2O
CHEBI_86360["CHEBI:86360"]
ing5_MnSO4_x_n_H2O -.-> CHEBI_86360
ing6_Diammonium_Citrate["Diammonium Citrate"]
CultureMech_002502 --> ing6_Diammonium_Citrate
CHEBI_63076["CHEBI:63076"]
ing6_Diammonium_Citrate -.-> CHEBI_63076
ing7_Tween_80["Tween 80"]
CultureMech_002502 --> ing7_Tween_80
CHEBI_53426["CHEBI:53426"]
ing7_Tween_80 -.-> CHEBI_53426
ing8_Tomato_juice["Tomato juice"]
CultureMech_002502 --> ing8_Tomato_juice
ing9_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
CultureMech_002502 --> ing9_L_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing9_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
Ingredients (10)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Casein peptone | 9.99001 G_PER_L | ( tryptic digest) | |
| 2 | Yeast extract | 4.99501 G_PER_L | ||
| 3 |
Glucose
C6H12O6 180.156 g/mol
|
9.99001 G_PER_L |
CHEBI:17234
glucose
|
|
| 4 | Fructose | 4.99501 G_PER_L |
CHEBI:28757
fructose
|
|
| 5 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.1998 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 6 |
MnSO4 x n H2O
Mn.O4S 151.001 g/mol
|
0.04995 G_PER_L |
CHEBI:86360
manganese(II) sulfate
|
|
| 7 |
Diammonium Citrate
C6H6O7.2H4N 226.185 g/mol
|
3.4965 G_PER_L |
CHEBI:63076
diammonium citrate
|
|
| 8 |
Tween 80
(C2H4O)w.(C2H4O)x.(C2H4O)y.(C2H4O)z.C24H44O6 604.822 g/mol
|
1 G_PER_L |
CHEBI:53426
polysorbate 80
|
|
| 9 | Tomato juice | 100 G_PER_L | ( filtered) | |
| 10 |
L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.499501 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
Preparation
- Adjust pH to 4.8.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:15.808592Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J143 |
| 2026-03-13T06:19:13.848221Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002502 |
| 2026-03-17T06:30:34.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:27.016338+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "LEUCONOSTOC OENOS MEDIUM" to "leuconostoc_oenos_medium" for consistent matching |
| 2026-06-05T07:40:16.695062+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:42.786368+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-10-07T14:33:27.670564+00:00 | codex | CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE | Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged. |