modified_nos_medium

Overview

External ontology

mediadive.medium:J144 (source link unavailable) — MODIFIED NOS MEDIUM

Applications

• Microbial cultivation

Notes

Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=144 Commercial Product: LB Medium (Luria-Bertani, Miller formulation) Supplier: Multiple suppliers (Difco, Sigma, etc.) Catalog: Multiple (L3522 Sigma, 244520 BD) Source: https://www.laboratorynotes.com/preparation-of-luria-bertani-lb-miller-broth/ Accessed: 2026-03-28 Constituent composition researched from product specification.

Composition

flowchart LR
    CultureMech_002503["modified_nos_medium"]
    ing0_Heart_Infusion_Broth["Heart Infusion Broth"]
    CultureMech_002503 --> ing0_Heart_Infusion_Broth
    ing1_Trypticase_peptone["Trypticase peptone"]
    CultureMech_002503 --> ing1_Trypticase_peptone
    ing2_Yeast_extract["Yeast extract"]
    CultureMech_002503 --> ing2_Yeast_extract
    ing3_Sodium_thioglycolate["Sodium thioglycolate"]
    CultureMech_002503 --> ing3_Sodium_thioglycolate
    CHEBI_86481["CHEBI:86481"]
    ing3_Sodium_thioglycolate -.-> CHEBI_86481
    ing4_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
    CultureMech_002503 --> ing4_L_Cysteine_HCl_x_H2O
    CHEBI_91248["CHEBI:91248"]
    ing4_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
    ing5_L_Asparagine["L-Asparagine"]
    CultureMech_002503 --> ing5_L_Asparagine
    CHEBI_17196["CHEBI:17196"]
    ing5_L_Asparagine -.-> CHEBI_17196
    ing6_Glucose["Glucose"]
    CultureMech_002503 --> ing6_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing6_Glucose -.-> CHEBI_17234
    ing7_Resazurin["Resazurin"]
    CultureMech_002503 --> ing7_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing7_Resazurin -.-> CHEBI_8806
    ing8_NaHCO3["NaHCO3"]
    CultureMech_002503 --> ing8_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing8_NaHCO3 -.-> CHEBI_32139
    ing9_Rabbit_serum["Rabbit serum"]
    CultureMech_002503 --> ing9_Rabbit_serum
    ing10_Thiamine_pyrophosphate["Thiamine pyrophosphate"]
    CultureMech_002503 --> ing10_Thiamine_pyrophosphate
    CHEBI_18290["CHEBI:18290"]
    ing10_Thiamine_pyrophosphate -.-> CHEBI_18290
    ing11_Isobutyric_acid["Isobutyric acid"]
    CultureMech_002503 --> ing11_Isobutyric_acid
    CHEBI_16135["CHEBI:16135"]
    ing11_Isobutyric_acid -.-> CHEBI_16135
    ing12_Tryptone["Tryptone"]
    CultureMech_002503 --> ing12_Tryptone
    ing13_Yeast_extract["Yeast extract"]
    CultureMech_002503 --> ing13_Yeast_extract
    ing14_Sodium_chloride["Sodium chloride"]
    CultureMech_002503 --> ing14_Sodium_chloride
    CHEBI_26710["CHEBI:26710"]
    ing14_Sodium_chloride -.-> CHEBI_26710
    ing15_Isovaleric_acid["Isovaleric acid"]
    CultureMech_002503 --> ing15_Isovaleric_acid
    CHEBI_28484["CHEBI:28484"]
    ing15_Isovaleric_acid -.-> CHEBI_28484
    ing16_Valeric_acid["Valeric acid"]
    CultureMech_002503 --> ing16_Valeric_acid
    CHEBI_17418["CHEBI:17418"]
    ing16_Valeric_acid -.-> CHEBI_17418
    ing17_NaOH["NaOH"]
    CultureMech_002503 --> ing17_NaOH
    CHEBI_32145["CHEBI:32145"]
    ing17_NaOH -.-> CHEBI_32145

Ingredients (18)

Preparation

1. Combine the above ingredients and bring to a boil in a round bottom flask while flushing with O2-free N2-H2-CO2 (85:10:5) gas mixture. Stopper anaerobically, wire or seal closed and autoclave at 121C for 20 min. After autoclaving, allow to cool, open without admitting oxygen and while flushing with gas, add the following ingredients which have been filter-sterilized ahead of time:
2. Mix together and filter-sterilize. Store at -20C.
3. Add volatile fatty acids to about 70-80 ml of NaOH solution and mix. Add NaOH solution to bring final volume to 100 ml. If not used the same day, filter-sterilize and store at 4C.

Target organisms

No target organisms are recorded.

Provenance