medium_for_treponema_pectinovorum (CultureMech:002504)

Overview

External ontology

mediadive.medium:J145 (source link unavailable) — MEDIUM FOR TREPONEMA PECTINOVORUM

Applications

Microbial cultivation

pH

7.1

Notes

Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=145

Composition

flowchart LR
    CultureMech_002504["medium_for_treponema_pectinovorum"]
    ing0_Pectin["Pectin"]
    CultureMech_002504 --> ing0_Pectin
    CHEBI_17309["CHEBI:17309"]
    ing0_Pectin -.-> CHEBI_17309
    ing1_K2HPO4["K2HPO4"]
    CultureMech_002504 --> ing1_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing1_K2HPO4 -.-> CHEBI_131527
    ing2_Polypeptone["Polypeptone"]
    CultureMech_002504 --> ing2_Polypeptone
    ing3_Heart_Infusion_Broth["Heart Infusion Broth"]
    CultureMech_002504 --> ing3_Heart_Infusion_Broth
    ing4_Yeast_extract["Yeast extract"]
    CultureMech_002504 --> ing4_Yeast_extract
    ing5_NaCl["NaCl"]
    CultureMech_002504 --> ing5_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing5_NaCl -.-> CHEBI_26710
    ing6__NH4_2SO4["(NH4)2SO4"]
    CultureMech_002504 --> ing6__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing6__NH4_2SO4 -.-> CHEBI_62946
    ing7_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
    CultureMech_002504 --> ing7_L_Cysteine_HCl_x_H2O
    CHEBI_91248["CHEBI:91248"]
    ing7_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
    ing8_Agar["Agar"]
    CultureMech_002504 --> ing8_Agar
    CHEBI_2509["CHEBI:2509"]
    ing8_Agar -.-> CHEBI_2509
    ing9_Rumen_fluid["Rumen fluid"]
    CultureMech_002504 --> ing9_Rumen_fluid
    ing10_Resazurin["Resazurin"]
    CultureMech_002504 --> ing10_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing10_Resazurin -.-> CHEBI_8806

Ingredients (11)

#	Ingredient	Concentration	Ontology
1	Pectin	0.796813 G_PER_L	CHEBI:17309 pectin
2	K2HPO4 HO4P.2K 174.174 g/mol	1.99203 G_PER_L	CHEBI:131527 dipotassium hydrogen phosphate
3	Polypeptone	4.98008 G_PER_L
4	Heart Infusion Broth	4.98008 G_PER_L
5	Yeast extract	4.98008 G_PER_L
6	NaCl Cl.Na 58.443 g/mol	4.98008 G_PER_L	CHEBI:26710 sodium chloride
7	(NH4)2SO4 2H4N.O4S 132.141 g/mol	1.99203 G_PER_L	CHEBI:62946 ammonium sulfate
8	L-Cysteine HCl x H2O C3H7NO2S.H2O.HCl 175.637 g/mol	0.677291 G_PER_L	CHEBI:91248 L-cysteine hydrochloride hydrate
9	Agar	0.996016 G_PER_L	CHEBI:2509 agar
10	Rumen fluid	500 G_PER_L
11	Resazurin C12H7NO4 229.191 g/mol	4 G_PER_L	CHEBI:8806 Resazurin

Preparation

Adjust pH to 7.0-7.2. Prepare medium anaerobically. Inoculate under a N2-CO2 (90:10) atmosphere.

Target organisms

No target organisms are recorded.

Provenance

Timestamp	Curator	Action	Notes
2026-01-26T17:53:15.813492Z	mediadive-import	Imported from MediaDive	Source: JCM, ID: J145
2026-03-13T06:19:13.861257Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:002504
2026-03-17T06:29:53.000000Z	mediaingredientmech-enrichment-v1.0	Added MediaIngredientMech links	Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:27.030449+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "MEDIUM FOR TREPONEMA PECTINOVORUM" to "medium_for_treponema_pectinovorum" for consistent matching
2026-06-05T07:41:30.157289+00:00	mim-legacy-to-chebi-migration-v1.0	Refreshed legacy MediaIngredientMech links to CHEBI keying	Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:06.463235+00:00	primary-term-label-canonicalization-v1.0	Canonicalized primary term label to OBO canonical (OAK)	2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-10-07T14:33:27.670564+00:00	codex	CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE	Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged.