jcm_medium_no_167

CultureMech:002526 bacterial COMPLEX SOLID_AGAR
Original name: JCM MEDIUM No. 167

Overview

External ontology
mediadive.medium:J167 (source link unavailable) — JCM MEDIUM No. 167
Applications
  • Microbial cultivation
pH
3.6
High-metal flag
True
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=167

Composition

flowchart LR
    CultureMech_002526["jcm_medium_no_167"]
    ing0_KH2PO4["KH2PO4"]
    CultureMech_002526 --> ing0_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing0_KH2PO4 -.-> CHEBI_63036
    ing1_KCl["KCl"]
    CultureMech_002526 --> ing1_KCl
    CHEBI_32588["CHEBI:32588"]
    ing1_KCl -.-> CHEBI_32588
    ing2_NH4Cl["NH4Cl"]
    CultureMech_002526 --> ing2_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing2_NH4Cl -.-> CHEBI_31206
    ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_002526 --> ing3_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing4_CaSO4_x_2_H2O["CaSO4 x 2 H2O"]
    CultureMech_002526 --> ing4_CaSO4_x_2_H2O
    CHEBI_32583["CHEBI:32583"]
    ing4_CaSO4_x_2_H2O -.-> CHEBI_32583
    ing5_NaCl["NaCl"]
    CultureMech_002526 --> ing5_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing5_NaCl -.-> CHEBI_26710
    ing6_Na_glutamate["Na glutamate"]
    CultureMech_002526 --> ing6_Na_glutamate
    CHEBI_64220["CHEBI:64220"]
    ing6_Na_glutamate -.-> CHEBI_64220
    ing7_Yeast_extract["Yeast extract"]
    CultureMech_002526 --> ing7_Yeast_extract
    ing8_Casamino_acids["Casamino acids"]
    CultureMech_002526 --> ing8_Casamino_acids
    ing9_Na2CO3["Na2CO3"]
    CultureMech_002526 --> ing9_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing9_Na2CO3 -.-> CHEBI_29377
    ing10_Agar["Agar"]
    CultureMech_002526 --> ing10_Agar
    CHEBI_2509["CHEBI:2509"]
    ing10_Agar -.-> CHEBI_2509
    ing11_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_002526 --> ing11_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing11_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing12_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_002526 --> ing12_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing12_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing13_H3BO3["H3BO3"]
    CultureMech_002526 --> ing13_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing13_H3BO3 -.-> CHEBI_33118
    ing14_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_002526 --> ing14_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing14_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing15_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_002526 --> ing15_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing15_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing16_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_002526 --> ing16_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing16_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing17_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_002526 --> ing17_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing17_Na2MoO4_x_2_H2O -.-> CHEBI_75213

Ingredients (18)

# Ingredient Concentration Ontology Notes
1 KH2PO4
H2O4P.K 136.084 g/mol
1 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
2 KCl
Cl.K 74.551 g/mol
1 G_PER_L CHEBI:32588
potassium chloride
3 NH4Cl
Cl.H4N 53.492 g/mol
1 G_PER_L CHEBI:31206
ammonium chloride
4 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.24 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
5 CaSO4 x 2 H2O
Ca.2H2O.O4S 172.171 g/mol
0.17 G_PER_L CHEBI:32583
calcium sulfate dihydrate
6 NaCl
Cl.Na 58.443 g/mol
200 G_PER_L CHEBI:26710
sodium chloride
7 Na glutamate
C5H8NO4.Na 169.112 g/mol
1 G_PER_L CHEBI:64220
monosodium glutamate
8 Yeast extract 5 G_PER_L
9 Casamino acids 5 G_PER_L
10 Na2CO3
CO3.2Na 105.988 g/mol
5 G_PER_L CHEBI:29377
sodium carbonate
11 Agar 20 G_PER_L CHEBI:2509
agar
12 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.1 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
13 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.03 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
14 H3BO3
H3BO3 61.833 g/mol
0.3 G_PER_L CHEBI:33118
boric acid
15 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.2 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
16 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.01 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
17 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.02 G_PER_L CHEBI:34887
nickel dichloride
18 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.03 G_PER_L CHEBI:75213
sodium molybdate dihydrate

Preparation

  1. Add components except Na2CO3 to distilled water and bring volume to 900 ml. Adjust pH to 6.5 and autoclave. Dissolve Na2CO3 in 100 ml distilled water and autoclave separately. Aseptically add the sterile Na2CO3 solution to the medium. Check final pH to be 9.0-9.5.
  2. Adjust pH to 3.6.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:15.863015Z mediadive-import Imported from MediaDive Source: JCM, ID: J167
2026-01-28T19:48:09.785507Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:19:13.969495Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002526
2026-03-17T06:29:45.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:27.152680+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "JCM MEDIUM No. 167" to "jcm_medium_no_167" for consistent matching
2026-06-05T07:41:09.835471+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 17 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-08T23:19:40.187072+00:00 chebi-regrounding-v1.0 Corrected CHEBI ingredient grounding (shared-id regrounding audit, G21/G22/G24) Part of the shared-id CHEBI regrounding audit (G21/G22/G24): one or more ingredient groundings on this record that had been collapsed onto a chemically non-specific or shared CHEBI id were re-grounded to the correct chemically-specific term, verified against OAK chebi.db rdfs:label. See reports/chebi_grounding_audit.md.
2026-06-10T07:47:00.191214+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.