halobacteria_medium

Overview

External ontology

mediadive.medium:J168 (source link unavailable) — HALOBACTERIA MEDIUM

Applications

• Microbial cultivation

pH range

{'min': 7.0, 'max': 7.2}

High-metal flag

True

Notes

Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=168 Source preparation excerpts: "Add components to distilled water and bring volume to 1.0 L."; "Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.". These instructions are not converted into an inferred step ordering. Separate JCM168 excerpts: "Adjust pH to 7.0-7.2."; "For preparation of solid medium, add 20.0 g/L agar.". The agar option is selected only for M160; liquid formulations remain unchanged.

Composition

flowchart LR
    CultureMech_002527["halobacteria_medium"]
    ing0_Casamino_acids["Casamino acids"]
    CultureMech_002527 --> ing0_Casamino_acids
    ing1_Yeast_extract["Yeast extract"]
    CultureMech_002527 --> ing1_Yeast_extract
    ing2_Na_glutamate["Na glutamate"]
    CultureMech_002527 --> ing2_Na_glutamate
    CHEBI_64220["CHEBI:64220"]
    ing2_Na_glutamate -.-> CHEBI_64220
    ing3_Trisodium_citrate["Trisodium citrate"]
    CultureMech_002527 --> ing3_Trisodium_citrate
    CHEBI_53258["CHEBI:53258"]
    ing3_Trisodium_citrate -.-> CHEBI_53258
    ing4_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_002527 --> ing4_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing4_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing5_KCl["KCl"]
    CultureMech_002527 --> ing5_KCl
    CHEBI_32588["CHEBI:32588"]
    ing5_KCl -.-> CHEBI_32588
    ing6_NaCl["NaCl"]
    CultureMech_002527 --> ing6_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing6_NaCl -.-> CHEBI_26710
    ing7_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
    CultureMech_002527 --> ing7_FeCl2_x_4_H2O
    CHEBI_86249["CHEBI:86249"]
    ing7_FeCl2_x_4_H2O -.-> CHEBI_86249
    ing8_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_002527 --> ing8_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing8_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing9_distilled_water["distilled water"]
    CultureMech_002527 --> ing9_distilled_water

Ingredients (10)

Preparation

1. Add components to distilled water and bring volume to 1.0 L.
2. Adjust pH to 7.0-7.2.
3. For preparation of solid medium, add 20.0 g/L agar.

Target organisms

No target organisms are recorded.

Provenance