jcm_medium_no_169

CultureMech:002528 bacterial COMPLEX SOLID_AGAR
Original name: JCM MEDIUM No. 169

Overview

External ontology
mediadive.medium:J169 (source link unavailable) — JCM MEDIUM No. 169
Applications
  • Microbial cultivation
pH
7.4
High-metal flag
True
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=169

Composition

flowchart LR
    CultureMech_002528["jcm_medium_no_169"]
    ing0_Casamino_acids["Casamino acids"]
    CultureMech_002528 --> ing0_Casamino_acids
    ing1_Yeast_extract["Yeast extract"]
    CultureMech_002528 --> ing1_Yeast_extract
    ing2_Trisodium_citrate["Trisodium citrate"]
    CultureMech_002528 --> ing2_Trisodium_citrate
    CHEBI_53258["CHEBI:53258"]
    ing2_Trisodium_citrate -.-> CHEBI_53258
    ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_002528 --> ing3_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing4_KCl["KCl"]
    CultureMech_002528 --> ing4_KCl
    CHEBI_32588["CHEBI:32588"]
    ing4_KCl -.-> CHEBI_32588
    ing5_NaCl["NaCl"]
    CultureMech_002528 --> ing5_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing5_NaCl -.-> CHEBI_26710
    ing6_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_002528 --> ing6_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing6_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing7_MnSO4_x_n_H2O["MnSO4 x n H2O"]
    CultureMech_002528 --> ing7_MnSO4_x_n_H2O
    CHEBI_86360["CHEBI:86360"]
    ing7_MnSO4_x_n_H2O -.-> CHEBI_86360
    ing8_Agar["Agar"]
    CultureMech_002528 --> ing8_Agar
    CHEBI_2509["CHEBI:2509"]
    ing8_Agar -.-> CHEBI_2509

Ingredients (9)

# Ingredient Concentration Ontology Notes
1 Casamino acids 7.5 G_PER_L
2 Yeast extract 10 G_PER_L
3 Trisodium citrate
C6H5O7.3Na 258.069 g/mol
3 G_PER_L CHEBI:53258
sodium citrate
4 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
20 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
5 KCl
Cl.K 74.551 g/mol
2 G_PER_L CHEBI:32588
potassium chloride
6 NaCl
Cl.Na 58.443 g/mol
250 G_PER_L CHEBI:26710
sodium chloride
7 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.05 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
8 MnSO4 x n H2O
Mn.O4S 151.001 g/mol
0.2 MG_PER_L CHEBI:86360
manganese(II) sulfate
9 Agar 20 G_PER_L CHEBI:2509
agar

Preparation

  1. Add components to distilled water and bring volume to 1.0 L.
  2. Adjust pH to 7.4.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:15.868848Z mediadive-import Imported from MediaDive Source: JCM, ID: J169
2026-01-28T19:48:24.176732Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:19:13.981717Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002528
2026-03-17T06:30:16.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:27.166229+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "JCM MEDIUM No. 169" to "jcm_medium_no_169" for consistent matching
2026-06-05T07:41:09.843658+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-08T23:19:40.195433+00:00 chebi-regrounding-v1.0 Corrected CHEBI ingredient grounding (audit G21/G22) Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI).
2026-06-10T07:47:00.195567+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.