lactobacillus_medium_ii

CultureMech:002529 bacterial COMPLEX SOLID_AGAR
Original name: LACTOBACILLUS MEDIUM II

Overview

External ontology
mediadive.medium:J16 (source link unavailable) — LACTOBACILLUS MEDIUM II
Applications
  • Microbial cultivation
pH
6.3
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=16

Composition

flowchart LR
    CultureMech_002529["lactobacillus_medium_ii"]
    ing0_Trypticase_peptone["Trypticase peptone"]
    CultureMech_002529 --> ing0_Trypticase_peptone
    ing1_Yeast_extract["Yeast extract"]
    CultureMech_002529 --> ing1_Yeast_extract
    ing2_Tryptose["Tryptose"]
    CultureMech_002529 --> ing2_Tryptose
    ing3_KH2PO4["KH2PO4"]
    CultureMech_002529 --> ing3_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing3_KH2PO4 -.-> CHEBI_63036
    ing4_K2HPO4["K2HPO4"]
    CultureMech_002529 --> ing4_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing4_K2HPO4 -.-> CHEBI_131527
    ing5_Tween_80["Tween 80"]
    CultureMech_002529 --> ing5_Tween_80
    CHEBI_53426["CHEBI:53426"]
    ing5_Tween_80 -.-> CHEBI_53426
    ing6_Sodium_acetate["Sodium acetate"]
    CultureMech_002529 --> ing6_Sodium_acetate
    CHEBI_32954["CHEBI:32954"]
    ing6_Sodium_acetate -.-> CHEBI_32954
    ing7_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
    CultureMech_002529 --> ing7_L_Cysteine_HCl_x_H2O
    CHEBI_91248["CHEBI:91248"]
    ing7_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
    ing8_Agar["Agar"]
    CultureMech_002529 --> ing8_Agar
    CHEBI_2509["CHEBI:2509"]
    ing8_Agar -.-> CHEBI_2509
    ing9_Glucose["Glucose"]
    CultureMech_002529 --> ing9_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing9_Glucose -.-> CHEBI_17234
    ing10_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_002529 --> ing10_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing10_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing11_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_002529 --> ing11_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing11_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing12_MnSO4_x_n_H2O["MnSO4 x n H2O"]
    CultureMech_002529 --> ing12_MnSO4_x_n_H2O
    CHEBI_86360["CHEBI:86360"]
    ing12_MnSO4_x_n_H2O -.-> CHEBI_86360

Ingredients (13)

# Ingredient Concentration Ontology Notes
1 Trypticase peptone 9.94036 G_PER_L
2 Yeast extract 4.97018 G_PER_L
3 Tryptose 2.98211 G_PER_L
4 KH2PO4
H2O4P.K 136.084 g/mol
2.98211 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
5 K2HPO4
HO4P.2K 174.174 g/mol
2.98211 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
6 Tween 80
(C2H4O)w.(C2H4O)x.(C2H4O)y.(C2H4O)z.C24H44O6 604.822 g/mol
1 G_PER_L CHEBI:53426
polysorbate 80
7 Sodium acetate
C2H3O2.Na 82.034 g/mol
0.994036 G_PER_L CHEBI:32954
sodium acetate
8 L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
0.198807 G_PER_L CHEBI:91248
L-cysteine hydrochloride hydrate
9 Agar 19.8807 G_PER_L CHEBI:2509
agar
10 Glucose
C6H12O6 180.156 g/mol
4.97018 G_PER_L CHEBI:17234
glucose
11 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
11.5 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
12 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.68 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
13 MnSO4 x n H2O
Mn.O4S 151.001 g/mol
2.4 G_PER_L CHEBI:86360
manganese(II) sulfate

Preparation

  1. Adjust pH to 6.3.
  2. Store at 4C.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:15.586139Z mediadive-import Imported from MediaDive Source: JCM, ID: J16
2026-03-13T06:19:13.987485Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002529
2026-03-17T06:30:37.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:27.172510+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "LACTOBACILLUS MEDIUM II" to "lactobacillus_medium_ii" for consistent matching
2026-06-05T07:40:16.776378+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 9 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:42.812672+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.