acidianus_brierleyi_medium

CultureMech:002536 archaea COMPLEX LIQUID
Original name: ACIDIANUS BRIERLEYI MEDIUM

Overview

External ontology
mediadive.medium:J176 (source link unavailable) — ACIDIANUS BRIERLEYI MEDIUM
Applications
  • Microbial cultivation
pH
2.0
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=176

Composition

flowchart LR
    CultureMech_002536["acidianus_brierleyi_medium"]
    ing0__NH4_2SO4["(NH4)2SO4"]
    CultureMech_002536 --> ing0__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing0__NH4_2SO4 -.-> CHEBI_62946
    ing1_KH2PO4["KH2PO4"]
    CultureMech_002536 --> ing1_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing1_KH2PO4 -.-> CHEBI_63036
    ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_002536 --> ing2_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing3_KCl["KCl"]
    CultureMech_002536 --> ing3_KCl
    CHEBI_32588["CHEBI:32588"]
    ing3_KCl -.-> CHEBI_32588
    ing4_Ca_NO3_2["Ca(NO3)2"]
    CultureMech_002536 --> ing4_Ca_NO3_2
    CHEBI_64205["CHEBI:64205"]
    ing4_Ca_NO3_2 -.-> CHEBI_64205
    ing5_Yeast_extract["Yeast extract"]
    CultureMech_002536 --> ing5_Yeast_extract
    ing6_Sulfur["Sulfur"]
    CultureMech_002536 --> ing6_Sulfur
    CHEBI_26833["CHEBI:26833"]
    ing6_Sulfur -.-> CHEBI_26833

Ingredients (7)

# Ingredient Concentration Ontology Notes
1 (NH4)2SO4
2H4N.O4S 132.141 g/mol
3 G_PER_L CHEBI:62946
ammonium sulfate
2 KH2PO4
H2O4P.K 136.084 g/mol
0.5 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
3 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.5 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
4 KCl
Cl.K 74.551 g/mol
0.1 G_PER_L CHEBI:32588
potassium chloride
5 Ca(NO3)2
Ca.2NO3 164.086 g/mol
0.01 G_PER_L CHEBI:64205
calcium nitrate
6 Yeast extract 0.2 G_PER_L
7 Sulfur
S 32.067 g/mol
10 G_PER_L CHEBI:26833
sulfur atom

Preparation

  1. Adjust pH to 2.0 with 10 N H2SO4. Autoclave yeast extract separately. Steam sulfur for 3 hr on each of 3 successive days.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:15.886678Z mediadive-import Imported from MediaDive Source: JCM, ID: J176
2026-02-06T05:10:22.257787Z invalid-chebi-removal Removed 1 invalid CHEBI ID(s) Removed CHEBI IDs with invalid format (7+ digits) from MicrobeMediaParam source data
2026-03-13T06:19:14.030551Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002536
2026-03-17T06:29:57.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:27.221526+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "ACIDIANUS BRIERLEYI MEDIUM" to "acidianus_brierleyi_medium" for consistent matching
2026-06-05T07:40:16.782611+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-08T23:19:27.283758+00:00 chebi-regrounding-v1.0 Corrected CHEBI ingredient grounding (audit G21/G22) Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI).
2026-06-10T07:46:42.815669+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-10-07T14:33:27.670564+00:00 codex CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged.