Overview
- External ontology
- mediadive.medium:J177 (source link unavailable) — 2.5% NaCl-BPG AGAR
- Applications
-
- Microbial cultivation
- pH
- 7.2
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=177
Composition
flowchart LR
CultureMech_002537["2_5_nacl_bpg_agar"]
ing0_Bacto_peptone["Bacto peptone"]
CultureMech_002537 --> ing0_Bacto_peptone
ing1_Fish_meat_extract["Fish meat extract"]
CultureMech_002537 --> ing1_Fish_meat_extract
ing2_Glucose["Glucose"]
CultureMech_002537 --> ing2_Glucose
CHEBI_17234["CHEBI:17234"]
ing2_Glucose -.-> CHEBI_17234
ing3_Agar["Agar"]
CultureMech_002537 --> ing3_Agar
CHEBI_2509["CHEBI:2509"]
ing3_Agar -.-> CHEBI_2509
ing4_NaCl["NaCl"]
CultureMech_002537 --> ing4_NaCl
CHEBI_26710["CHEBI:26710"]
ing4_NaCl -.-> CHEBI_26710
ing5_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_002537 --> ing5_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing5_MgSO4_x_7_H2O -.-> CHEBI_31795
ing6_KCl["KCl"]
CultureMech_002537 --> ing6_KCl
CHEBI_32588["CHEBI:32588"]
ing6_KCl -.-> CHEBI_32588
Ingredients (7)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Bacto peptone | 5 G_PER_L | ||
| 2 | Fish meat extract | 5 G_PER_L | ||
| 3 |
Glucose
C6H12O6 180.156 g/mol
|
1 G_PER_L |
CHEBI:17234
glucose
|
|
| 4 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
|
| 5 |
NaCl
Cl.Na 58.443 g/mol
|
25 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 6 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
2.5 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 7 |
KCl
Cl.K 74.551 g/mol
|
1 G_PER_L |
CHEBI:32588
potassium chloride
|
Preparation
- Adjust pH to 7.2.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:15.888788Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J177 |
| 2026-03-13T06:19:14.034604Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002537 |
| 2026-03-17T06:29:42.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:27.226037+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "2.5% NaCl-BPG AGAR" to "2_5_nacl_bpg_agar" for consistent matching |
| 2026-06-05T07:40:15.722155+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-10-07T14:33:27.670564+00:00 | codex | CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE | Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged. |