modified_horikoshi_i_medium

CultureMech:002543 bacterial COMPLEX SOLID_AGAR
Original name: MODIFIED HORIKOSHI-I MEDIUM

Overview

External ontology
mediadive.medium:J182 (source link unavailable) — MODIFIED HORIKOSHI-I MEDIUM
Applications
  • Microbial cultivation
pH
9.0
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=182

Composition

flowchart LR
    CultureMech_002543["modified_horikoshi_i_medium"]
    ing0_Glucose["Glucose"]
    CultureMech_002543 --> ing0_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing0_Glucose -.-> CHEBI_17234
    ing1_Polypeptone["Polypeptone"]
    CultureMech_002543 --> ing1_Polypeptone
    ing2_Yeast_extract["Yeast extract"]
    CultureMech_002543 --> ing2_Yeast_extract
    ing3_NaCl["NaCl"]
    CultureMech_002543 --> ing3_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing3_NaCl -.-> CHEBI_26710
    ing4_K2HPO4["K2HPO4"]
    CultureMech_002543 --> ing4_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing4_K2HPO4 -.-> CHEBI_131527
    ing5_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_002543 --> ing5_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing5_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing6_Agar["Agar"]
    CultureMech_002543 --> ing6_Agar
    CHEBI_2509["CHEBI:2509"]
    ing6_Agar -.-> CHEBI_2509

Ingredients (7)

# Ingredient Concentration Ontology Notes
1 Glucose
C6H12O6 180.156 g/mol
11.1111 G_PER_L CHEBI:17234
glucose
2 Polypeptone 5.55556 G_PER_L
3 Yeast extract 5.55556 G_PER_L
4 NaCl
Cl.Na 58.443 g/mol
11.1111 G_PER_L CHEBI:26710
sodium chloride
5 K2HPO4
HO4P.2K 174.174 g/mol
1.11111 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
6 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.222222 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
7 Agar 16.6667 G_PER_L CHEBI:2509
agar

Preparation

  1. After autoclaving, aseptically add 100 ml of a sterile 10% NaHCO3 solution to the medium. Check final pH to be about 9.0.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:15.896706Z mediadive-import Imported from MediaDive Source: JCM, ID: J182
2026-03-13T06:19:14.053251Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002543
2026-03-17T06:29:49.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:27.247460+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "MODIFIED HORIKOSHI-I MEDIUM" to "modified_horikoshi_i_medium" for consistent matching
2026-06-05T07:41:36.750361+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:08.671340+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.