Overview
- External ontology
- mediadive.medium:J185 (source link unavailable) — JCM MEDIUM No. 185
- Applications
-
- Microbial cultivation
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=185
Composition
flowchart LR
CultureMech_002546["jcm_medium_no_185"]
ing0_Yeast_extract["Yeast extract"]
CultureMech_002546 --> ing0_Yeast_extract
ing1_Sulfur["Sulfur"]
CultureMech_002546 --> ing1_Sulfur
CHEBI_26833["CHEBI:26833"]
ing1_Sulfur -.-> CHEBI_26833
ing2__NH4_2SO4["(NH4)2SO4"]
CultureMech_002546 --> ing2__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing2__NH4_2SO4 -.-> CHEBI_62946
ing3_KH2PO4["KH2PO4"]
CultureMech_002546 --> ing3_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing3_KH2PO4 -.-> CHEBI_63036
ing4_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_002546 --> ing4_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing4_MgSO4_x_7_H2O -.-> CHEBI_31795
ing5_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_002546 --> ing5_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing5_CaCl2_x_2_H2O -.-> CHEBI_86158
ing6_FeCl3_x_6_H2O["FeCl3 x 6 H2O"]
CultureMech_002546 --> ing6_FeCl3_x_6_H2O
CHEBI_86254["CHEBI:86254"]
ing6_FeCl3_x_6_H2O -.-> CHEBI_86254
ing7_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_002546 --> ing7_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing7_MnCl2_x_4_H2O -.-> CHEBI_86368
ing8_Na2B4O7_x_10_H2O["Na2B4O7 x 10 H2O"]
CultureMech_002546 --> ing8_Na2B4O7_x_10_H2O
CHEBI_131366["CHEBI:131366"]
ing8_Na2B4O7_x_10_H2O -.-> CHEBI_131366
ing9_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_002546 --> ing9_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing9_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing10_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_002546 --> ing10_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing10_CuCl2_x_2_H2O -.-> CHEBI_86318
ing11_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_002546 --> ing11_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing11_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing12_VOSO4_x_n_H2O["VOSO4 x n H2O"]
CultureMech_002546 --> ing12_VOSO4_x_n_H2O
CHEBI_87020["CHEBI:87020"]
ing12_VOSO4_x_n_H2O -.-> CHEBI_87020
ing13_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
CultureMech_002546 --> ing13_CoSO4_x_7_H2O
CHEBI_91244["CHEBI:91244"]
ing13_CoSO4_x_7_H2O -.-> CHEBI_91244
Ingredients (14)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Yeast extract | 0.5 G_PER_L | Copied from referenced JCM Medium 174 | |
| 2 |
Sulfur
S 32.067 g/mol
|
1 G_PER_L |
CHEBI:26833
sulfur atom
|
Copied from referenced JCM Medium 174 |
| 3 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
1.3 G_PER_L |
CHEBI:62946
ammonium sulfate
|
Copied from referenced JCM Medium 174 |
| 4 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.28 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Copied from referenced JCM Medium 174 |
| 5 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.25 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Copied from referenced JCM Medium 174 |
| 6 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.07 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Copied from referenced JCM Medium 174 |
| 7 |
FeCl3 x 6 H2O
Cl3Fe.6H2O 270.294 g/mol
|
0.002 G_PER_L |
CHEBI:86254
iron trichloride hexahydrate
|
Copied from referenced JCM Medium 174 |
| 8 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.0018 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
Copied from referenced JCM Medium 174 |
| 9 |
Na2B4O7 x 10 H2O
10H2O.H4B4O9.2Na 417.401 g/mol
|
0.0045 G_PER_L |
CHEBI:131366
disodium tetraborate decahydrate
|
Copied from referenced JCM Medium 174 |
| 10 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.00022 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
Copied from referenced JCM Medium 174 |
| 11 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
5e-05 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
Copied from referenced JCM Medium 174 |
| 12 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
3e-05 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Copied from referenced JCM Medium 174 |
| 13 |
VOSO4 x n H2O
(H2O)n.O5SV 181.021 g/mol
|
3e-05 G_PER_L |
CHEBI:87020
vanadyl sulfate hydrate
|
Copied from referenced JCM Medium 174 |
| 14 |
CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
|
1e-05 G_PER_L |
CHEBI:91244
cobalt(2+) sulfate heptahydrate
|
Copied from referenced JCM Medium 174 |
Preparation
- Use Medium No. 174 with 0.02 g/L (final) yeast extract.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:15.905406Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J185 |
| 2026-02-03T11:26:54.061367 | quality-tagger-v1.0 | Added data quality flag | Marked as incomplete_composition due to placeholder ingredients |
| 2026-03-13T06:19:14.071632Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002546 |
| 2026-03-17T19:45:27.268847+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "JCM MEDIUM No. 185" to "jcm_medium_no_185" for consistent matching |
| 2026-04-04T18:49:19.163164 | copy_referenced_compositions | RESOLVED_REFERENCE | Resolved reference by copying from acidianus_infernus_medium_a (CultureMech:002534) |
| 2026-05-16T08:57:36.174957+00:00 | migrate_data_quality_flags.py | MIGRATED_DATA_QUALITY_FLAGS | dict(2 keys) -> list(1 entries) |
| 2026-06-05T07:41:09.852485+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 12 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:00.198383+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |