Overview
- External ontology
- mediadive.medium:J194 (source link unavailable) — MMYS-II MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.8
- High-metal flag
- True
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=194
Composition
flowchart LR
CultureMech_002556["mmys_ii_medium"]
ing0__NH4_2SO4["(NH4)2SO4"]
CultureMech_002556 --> ing0__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing0__NH4_2SO4 -.-> CHEBI_62946
ing1_NaCl["NaCl"]
CultureMech_002556 --> ing1_NaCl
CHEBI_26710["CHEBI:26710"]
ing1_NaCl -.-> CHEBI_26710
ing2_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_002556 --> ing2_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing2_MgCl2_x_6_H2O -.-> CHEBI_86345
ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_002556 --> ing3_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
ing4_Sodium_DL_malate["Sodium DL-malate"]
CultureMech_002556 --> ing4_Sodium_DL_malate
CHEBI_91260["CHEBI:91260"]
ing4_Sodium_DL_malate -.-> CHEBI_91260
ing5_Yeast_extract["Yeast extract"]
CultureMech_002556 --> ing5_Yeast_extract
ing6_Na2S2O3_x_5_H2O["Na2S2O3 x 5 H2O"]
CultureMech_002556 --> ing6_Na2S2O3_x_5_H2O
CHEBI_32150["CHEBI:32150"]
ing6_Na2S2O3_x_5_H2O -.-> CHEBI_32150
ing7_Potassium_phosphate_buffer["Potassium phosphate buffer"]
CultureMech_002556 --> ing7_Potassium_phosphate_buffer
ing8_Na2_EDTA["Na2-EDTA"]
CultureMech_002556 --> ing8_Na2_EDTA
CHEBI_64734["CHEBI:64734"]
ing8_Na2_EDTA -.-> CHEBI_64734
ing9_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
CultureMech_002556 --> ing9_FeCl2_x_4_H2O
CHEBI_86249["CHEBI:86249"]
ing9_FeCl2_x_4_H2O -.-> CHEBI_86249
ing10_ZnCl2["ZnCl2"]
CultureMech_002556 --> ing10_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing10_ZnCl2 -.-> CHEBI_49976
ing11_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_002556 --> ing11_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing11_MnCl2_x_4_H2O -.-> CHEBI_86368
ing12_H3BO3["H3BO3"]
CultureMech_002556 --> ing12_H3BO3
CHEBI_33118["CHEBI:33118"]
ing12_H3BO3 -.-> CHEBI_33118
ing13_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_002556 --> ing13_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing13_CoCl2_x_6_H2O -.-> CHEBI_53503
ing14_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_002556 --> ing14_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing14_CuCl2_x_2_H2O -.-> CHEBI_86318
ing15_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_002556 --> ing15_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing15_NiCl2_x_6_H2O -.-> CHEBI_34887
ing16_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_002556 --> ing16_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing16_Na2MoO4_x_2_H2O -.-> CHEBI_75213
Ingredients (17)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
1.99601 G_PER_L |
CHEBI:62946
ammonium sulfate
|
|
| 2 |
NaCl
Cl.Na 58.443 g/mol
|
16.3673 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 3 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.399202 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 4 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.0998004 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 5 | Sodium DL-malate | 7.18563 G_PER_L |
CHEBI:91260
disodium malate
|
|
| 6 | Yeast extract | 1.99601 G_PER_L | ||
| 7 |
Na2S2O3 x 5 H2O
5H2O.2Na.O3S2 248.186 g/mol
|
0.998004 G_PER_L |
CHEBI:32150
sodium thiosulfate pentahydrate
|
|
| 8 | Potassium phosphate buffer | 500 G_PER_L | ||
| 9 |
Na2-EDTA
C10H14N2O8.2Na 336.208 g/mol
|
5.2 G_PER_L |
CHEBI:64734
EDTA disodium salt (anhydrous)
|
|
| 10 |
FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
|
1.5 G_PER_L |
CHEBI:86249
iron dichloride tetrahydrate
|
|
| 11 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.07 G_PER_L |
CHEBI:49976
zinc dichloride
|
|
| 12 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.1 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 13 |
H3BO3
H3BO3 61.833 g/mol
|
0.062 G_PER_L |
CHEBI:33118
boric acid
|
|
| 14 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.19 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 15 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.017 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 16 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.024 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 17 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.036 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Preparation
- Separately autoclave Solutions I and II. Mix aseptically and adjust final pH to 7.7-7.8.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:15.926304Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J194 |
| 2026-03-13T06:19:14.131747Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002556 |
| 2026-03-17T06:30:44.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:27.338599+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MMYS-II MEDIUM" to "mmys_ii_medium" for consistent matching |
| 2026-06-05T07:41:35.822171+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 15 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:08.393717+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |