Overview
- External ontology
- mediadive.medium:J195 (source link unavailable) — THERMOPROTEUS NEUTROPHILUS MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 6.5
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=195
Composition
flowchart LR
CultureMech_002557["thermoproteus_neutrophilus_medium"]
ing0_NaHCO3["NaHCO3"]
CultureMech_002557 --> ing0_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing0_NaHCO3 -.-> CHEBI_32139
ing1_Yeast_extract["Yeast extract"]
CultureMech_002557 --> ing1_Yeast_extract
ing2_Sodium_dithionite["Sodium dithionite"]
CultureMech_002557 --> ing2_Sodium_dithionite
CHEBI_66870["CHEBI:66870"]
ing2_Sodium_dithionite -.-> CHEBI_66870
ing3_Sulfur["Sulfur"]
CultureMech_002557 --> ing3_Sulfur
CHEBI_26833["CHEBI:26833"]
ing3_Sulfur -.-> CHEBI_26833
ing4_Resazurin["Resazurin"]
CultureMech_002557 --> ing4_Resazurin
CHEBI_8806["CHEBI:8806"]
ing4_Resazurin -.-> CHEBI_8806
ing5__NH4_2SO4["(NH4)2SO4"]
CultureMech_002557 --> ing5__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing5__NH4_2SO4 -.-> CHEBI_62946
ing6_KH2PO4["KH2PO4"]
CultureMech_002557 --> ing6_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing6_KH2PO4 -.-> CHEBI_63036
ing7_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_002557 --> ing7_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing7_MgSO4_x_7_H2O -.-> CHEBI_31795
ing8_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_002557 --> ing8_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing8_CaCl2_x_2_H2O -.-> CHEBI_86158
ing9_FeCl3_x_6_H2O["FeCl3 x 6 H2O"]
CultureMech_002557 --> ing9_FeCl3_x_6_H2O
CHEBI_86254["CHEBI:86254"]
ing9_FeCl3_x_6_H2O -.-> CHEBI_86254
ing10_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_002557 --> ing10_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing10_MnCl2_x_4_H2O -.-> CHEBI_86368
ing11_Na2B4O7_x_10_H2O["Na2B4O7 x 10 H2O"]
CultureMech_002557 --> ing11_Na2B4O7_x_10_H2O
CHEBI_131366["CHEBI:131366"]
ing11_Na2B4O7_x_10_H2O -.-> CHEBI_131366
ing12_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_002557 --> ing12_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing12_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing13_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_002557 --> ing13_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing13_CuCl2_x_2_H2O -.-> CHEBI_86318
ing14_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_002557 --> ing14_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing14_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing15_VOSO4_x_n_H2O["VOSO4 x n H2O"]
CultureMech_002557 --> ing15_VOSO4_x_n_H2O
CHEBI_87020["CHEBI:87020"]
ing15_VOSO4_x_n_H2O -.-> CHEBI_87020
ing16_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
CultureMech_002557 --> ing16_CoSO4_x_7_H2O
CHEBI_91244["CHEBI:91244"]
ing16_CoSO4_x_7_H2O -.-> CHEBI_91244
Ingredients (17)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaHCO3
CHO3.Na 84.006 g/mol
|
0.85 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 2 | Yeast extract | 0.2 G_PER_L | ||
| 3 |
Sodium dithionite
2Na.O4S2 174.11 g/mol
|
0.025 G_PER_L |
CHEBI:66870
sodium dithionite
|
|
| 4 |
Sulfur
S 32.067 g/mol
|
8 G_PER_L |
CHEBI:26833
sulfur atom
|
|
| 5 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.0004 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 6 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
1.3 G_PER_L |
CHEBI:62946
ammonium sulfate
|
|
| 7 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.28 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 8 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.25 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 9 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.07 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 10 |
FeCl3 x 6 H2O
Cl3Fe.6H2O 270.294 g/mol
|
0.002 G_PER_L |
CHEBI:86254
iron trichloride hexahydrate
|
|
| 11 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.0018 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 12 |
Na2B4O7 x 10 H2O
10H2O.H4B4O9.2Na 417.401 g/mol
|
0.0045 G_PER_L |
CHEBI:131366
disodium tetraborate decahydrate
|
|
| 13 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.00022 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 14 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
5e-05 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 15 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
3e-05 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 16 |
VOSO4 x n H2O
(H2O)n.O5SV 181.021 g/mol
|
3e-05 G_PER_L |
CHEBI:87020
vanadyl sulfate hydrate
|
|
| 17 |
CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
|
1e-05 G_PER_L |
CHEBI:91244
cobalt(2+) sulfate heptahydrate
|
Preparation
- Mix ingredients, except yeast extract, sodium dithionite and sulfur, and adjust pH to 6.5. Autoclave, then cool under H2-CO2 (80:20) gas mixture. Separately autoclave yeast extract as a 10% solution under a N2 atmosphere. Filter-sterilize NaHCO3 as an 8% solution while gassing with N2. Steam sulfur for 3 hr on each of 3 successive days. Aseptically and anaerobically add the yeast extract and NaHCO3 solutions, and sulfur to the medium under a H2-CO2 (80:20) atmosphere. Readjust pH to 6.5 by further gassing with H2-CO2 (80:20) gas mixture, if necessary. Prior to inoculation, add filter-sterilized 2.5% sodium dithionite solution. Pressurize inoculated bottles to 100 kPa H2-CO2 (80:20).
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:15.929132Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J195 |
| 2026-03-13T06:19:14.139874Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002557 |
| 2026-03-17T06:30:28.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:27.348020+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "THERMOPROTEUS NEUTROPHILUS MEDIUM" to "thermoproteus_neutrophilus_medium" for consistent matching |
| 2026-06-05T07:41:50.463527+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 15 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:13.134314+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |