Overview
- External ontology
- mediadive.medium:J197 (source link unavailable) — JCM MEDIUM No. 197
- Applications
-
- Microbial cultivation
- pH
- 6.8
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=197
Composition
flowchart LR
CultureMech_002559["jcm_medium_no_197"]
ing0_KH2PO4["KH2PO4"]
CultureMech_002559 --> ing0_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing0_KH2PO4 -.-> CHEBI_63036
ing1_K2HPO4["K2HPO4"]
CultureMech_002559 --> ing1_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing1_K2HPO4 -.-> CHEBI_131527
ing2_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_002559 --> ing2_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing2_MgCl2_x_6_H2O -.-> CHEBI_86345
ing3_NH4Cl["NH4Cl"]
CultureMech_002559 --> ing3_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing3_NH4Cl -.-> CHEBI_31206
ing4_Yeast_extract["Yeast extract"]
CultureMech_002559 --> ing4_Yeast_extract
ing5_Polypeptone["Polypeptone"]
CultureMech_002559 --> ing5_Polypeptone
ing6_Sodium_acetate["Sodium acetate"]
CultureMech_002559 --> ing6_Sodium_acetate
CHEBI_32954["CHEBI:32954"]
ing6_Sodium_acetate -.-> CHEBI_32954
ing7_Trimethylamine_HCl["Trimethylamine-HCl"]
CultureMech_002559 --> ing7_Trimethylamine_HCl
CHEBI_64700["CHEBI:64700"]
ing7_Trimethylamine_HCl -.-> CHEBI_64700
ing8_Resazurin["Resazurin"]
CultureMech_002559 --> ing8_Resazurin
CHEBI_8806["CHEBI:8806"]
ing8_Resazurin -.-> CHEBI_8806
ing9_Distilled_water["Distilled water"]
CultureMech_002559 --> ing9_Distilled_water
Ingredients (10)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
KH2PO4
H2O4P.K 136.084 g/mol
|
CHEBI:63036
potassium dihydrogen phosphate
|
Source batch input: 0.75 g. Final preparation volume is not independently established; the unsupported g/L assertion is withdrawn, not replaced by an assumed one-liter concentration. | |
| 2 |
K2HPO4
HO4P.2K 174.174 g/mol
|
CHEBI:131527
dipotassium hydrogen phosphate
|
Source batch input: 0.75 g. Final preparation volume is not independently established; the unsupported g/L assertion is withdrawn, not replaced by an assumed one-liter concentration. | |
| 3 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
CHEBI:86345
magnesium dichloride hexahydrate
|
Source batch input: 0.36 g. Final preparation volume is not independently established; the unsupported g/L assertion is withdrawn, not replaced by an assumed one-liter concentration. | |
| 4 |
NH4Cl
Cl.H4N 53.492 g/mol
|
CHEBI:31206
ammonium chloride
|
Source batch input: 1 g. Final preparation volume is not independently established; the unsupported g/L assertion is withdrawn, not replaced by an assumed one-liter concentration. | |
| 5 | Yeast extract | Source batch input: 0.1 g. Final preparation volume is not independently established; the unsupported g/L assertion is withdrawn, not replaced by an assumed one-liter concentration. Source qualifier: BD-Difco. | ||
| 6 | Polypeptone | Source batch input: 0.1 g. Final preparation volume is not independently established; the unsupported g/L assertion is withdrawn, not replaced by an assumed one-liter concentration. Source qualifier: Nihon Pharm. Co.. | ||
| 7 |
Sodium acetate
C2H3O2.Na 82.034 g/mol
|
CHEBI:32954
sodium acetate
|
Source batch input: 2.5 g. Final preparation volume is not independently established; the unsupported g/L assertion is withdrawn, not replaced by an assumed one-liter concentration. | |
| 8 |
Trimethylamine-HCl
C3H9N.HCl 95.573 g/mol
|
CHEBI:64700
trimethylamine hydrochloride
|
Source batch input: 8 g. Final preparation volume is not independently established; the unsupported g/L assertion is withdrawn, not replaced by an assumed one-liter concentration. | |
| 9 |
Resazurin
C12H7NO4 229.191 g/mol
|
CHEBI:8806
Resazurin
|
Source batch input: 1 mg. Final preparation volume is not independently established; the unsupported g/L assertion is withdrawn, not replaced by an assumed one-liter concentration. | |
| 10 | Distilled water | 0.981 L | Source water batch input: 981 ml = 0.981 L. This is not a measured final volume. Prior duplicate-collapse notes describe the superseded flattened record. |
Preparation
- Mix ingredients except NaHCO3, Trace vitamins, Na2S x 9H2O and cysteine x HCl x H2O, and autoclave under a N2-CO2 (80:20) atmosphere. Filter-sterilize NaHCO3 as an 8% solution and Trace vitamins while gassing with N2. Separately autoclave Na2S x 9H2O and cysteine x HCl x H2O as 5% solutions under a N2 atmosphere. Aseptically and anaerobically add NaHCO3 and Trace vitamins to the medium under a N2-CO2 (80:20) atmosphere. Prior to inoculation, add the sterile Na2S x 9H2O and cysteine x HCl x H2O solutions, and adjust pH to 6.8.
Solutions
Trace mineral solution
Dose: 0.009 L
| Ingredient | Concentration | Notes |
|---|---|---|
| Nitrilotriacetic acid | 4.5 G_PER_L | Source stock batch input: 4.5 g. Retain provider-declared stock g_l=4.5 only, not a final-medium concentration or an independently verified final stock volume. Prior duplicate-merge notes describe the superseded flattened record. |
| FeCl2 x 4 H2O | 0.4 G_PER_L | Source stock batch input: 0.4 g. Retain provider-declared stock g_l=0.4 only, not a final-medium concentration or an independently verified final stock volume. Prior duplicate-merge notes describe the superseded flattened record. |
| MnCl2 x 4 H2O | 0.1 G_PER_L | Source stock batch input: 0.1 g. Retain provider-declared stock g_l=0.1 only, not a final-medium concentration or an independently verified final stock volume. Prior duplicate-merge notes describe the superseded flattened record. |
| CoCl2 x 6 H2O | 0.17 G_PER_L | Source stock batch input: 0.17 g. Retain provider-declared stock g_l=0.17 only, not a final-medium concentration or an independently verified final stock volume. Prior duplicate-merge notes describe the superseded flattened record. |
| ZnCl2 | 0.1 G_PER_L | Source stock batch input: 0.1 g. Retain provider-declared stock g_l=0.1 only, not a final-medium concentration or an independently verified final stock volume. Prior duplicate-merge notes describe the superseded flattened record. |
| CaCl2 x 2 H2O | 0.02 G_PER_L | Source stock batch input: 0.02 g. Retain provider-declared stock g_l=0.02 only, not a final-medium concentration or an independently verified final stock volume. Prior duplicate-merge notes describe the superseded flattened record. |
| H3BO3 | 0.019 G_PER_L | Source stock batch input: 0.019 g. Retain provider-declared stock g_l=0.019 only, not a final-medium concentration or an independently verified final stock volume. Prior duplicate-merge notes describe the superseded flattened record. |
| Na2MoO4 x 2 H2O | 0.01 G_PER_L | Source stock batch input: 0.01 g. Retain provider-declared stock g_l=0.01 only, not a final-medium concentration or an independently verified final stock volume. Prior duplicate-merge notes describe the superseded flattened record. |
| NiCl2 x 6 H2O | 0.03 G_PER_L | Source stock batch input: 0.03 g. Retain provider-declared stock g_l=0.03 only, not a final-medium concentration or an independently verified final stock volume. Prior duplicate-merge notes describe the superseded flattened record. |
| Distilled water | 1 L | Source water batch input: 1000 ml = 1 L. This is not a measured final volume. Prior duplicate-collapse notes describe the superseded flattened record. |
| KOH solution | Source-explicit pH adjustment with no fixed dose. Strength, when stated as 1 N, is retained in preparation text; no molarity is inferred. |
Source stock addition: 9 ml = 0.009 L per receiving batch, not grams per liter or a dose per final liter.
Trace vitamins
Dose: 0.01 L
| Ingredient | Concentration | Notes |
|---|---|---|
| Biotin | 0.002 G_PER_L | Source stock batch input: 2 mg. Retain provider-declared stock g_l=0.002 only, not a final-medium concentration or an independently verified final stock volume. Prior duplicate-merge notes describe the superseded flattened record. |
| Folic acid | 0.002 G_PER_L | Source stock batch input: 2 mg. Retain provider-declared stock g_l=0.002 only, not a final-medium concentration or an independently verified final stock volume. Prior duplicate-merge notes describe the superseded flattened record. |
| Pyridoxine hydrochloride | 0.01 G_PER_L | Source stock batch input: 10 mg. Retain provider-declared stock g_l=0.01 only, not a final-medium concentration or an independently verified final stock volume. Prior duplicate-merge notes describe the superseded flattened record. |
| Thiamine HCl | 0.005 G_PER_L | Source stock batch input: 5 mg. Retain provider-declared stock g_l=0.005 only, not a final-medium concentration or an independently verified final stock volume. Prior duplicate-merge notes describe the superseded flattened record. |
| Riboflavin | 0.005 G_PER_L | Source stock batch input: 5 mg. Retain provider-declared stock g_l=0.005 only, not a final-medium concentration or an independently verified final stock volume. Prior duplicate-merge notes describe the superseded flattened record. |
| Nicotinic acid | 0.005 G_PER_L | Source stock batch input: 5 mg. Retain provider-declared stock g_l=0.005 only, not a final-medium concentration or an independently verified final stock volume. Prior duplicate-merge notes describe the superseded flattened record. |
| Calcium pantothenate | 0.005 G_PER_L | Source stock batch input: 5 mg. Retain provider-declared stock g_l=0.005 only, not a final-medium concentration or an independently verified final stock volume. Prior duplicate-merge notes describe the superseded flattened record. |
| Vitamin B12 | 0.0001 G_PER_L | Source stock batch input: 0.1 mg. Retain provider-declared stock g_l=0.0001 only, not a final-medium concentration or an independently verified final stock volume. Prior duplicate-merge notes describe the superseded flattened record. |
| p-Aminobenzoic acid | 0.005 G_PER_L | Source stock batch input: 5 mg. Retain provider-declared stock g_l=0.005 only, not a final-medium concentration or an independently verified final stock volume. Prior duplicate-merge notes describe the superseded flattened record. |
| Lipoic acid | 0.005 G_PER_L | Source stock batch input: 5 mg. Retain provider-declared stock g_l=0.005 only, not a final-medium concentration or an independently verified final stock volume. Prior duplicate-merge notes describe the superseded flattened record. |
| Distilled water | 1 L | Source water batch input: 1000 ml = 1 L. This is not a measured final volume. Prior duplicate-collapse notes describe the superseded flattened record. |
Source stock addition: 10 ml = 0.01 L per receiving batch, not grams per liter or a dose per final liter.
NaHCO3 separately prepared solution
| Ingredient | Concentration | Notes |
|---|---|---|
| NaHCO3 | Source batch input: 0.8 g. Final preparation volume is not independently established; the unsupported g/L assertion is withdrawn, not replaced by an assumed one-liter concentration. This is the contribution specified for the receiving medium batch, not the mass for preparing an unspecified volume of stock. |
The source specifies a separate percentage-labelled preparation but does not establish percentage basis or its addition volume. No numeric stock strength or dose is asserted. Composition notes record the receiving-batch contribution only.
Na2S x 9 H2O separately prepared solution
| Ingredient | Concentration | Notes |
|---|---|---|
| Na2S x 9 H2O | Source batch input: 0.5 g. Final preparation volume is not independently established; the unsupported g/L assertion is withdrawn, not replaced by an assumed one-liter concentration. This is the contribution specified for the receiving medium batch, not the mass for preparing an unspecified volume of stock. Unresolved source identity discrepancy: the original JCM197 table spells NaS2, while its preparation spells Na2S. MediaDive normalizes the table to Na2S; TOGO retains NaS2. The existing label/grounding is preserved, not recertified, and no numeric sulfide concentration is asserted. |
The source specifies a separate percentage-labelled preparation but does not establish percentage basis or its addition volume. No numeric stock strength or dose is asserted. Composition notes record the receiving-batch contribution only.
L-Cysteine HCl x H2O separately prepared solution
| Ingredient | Concentration | Notes |
|---|---|---|
| L-Cysteine HCl x H2O | Source batch input: 0.5 g. Final preparation volume is not independently established; the unsupported g/L assertion is withdrawn, not replaced by an assumed one-liter concentration. This is the contribution specified for the receiving medium batch, not the mass for preparing an unspecified volume of stock. |
The source specifies a separate percentage-labelled preparation but does not establish percentage basis or its addition volume. No numeric stock strength or dose is asserted. Composition notes record the receiving-batch contribution only.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:15.935661Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J197 |
| 2026-01-28T19:48:08.230510Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:19:14.159562Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002559 |
| 2026-03-17T06:29:42.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:27.370295+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "JCM MEDIUM No. 197" to "jcm_medium_no_197" for consistent matching |
| 2026-06-05T07:41:09.887031+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 29 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:00.208963+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-10-08T13:52:57.998884+00:00 | codex | RESTORED_JCM_STOCK_PREPARATION_BOUNDARIES_WITH_EVIDENCE | Restored preparation boundaries and explicit stock-dose bases. Preserved source-specific conflicts, chemical annotations and earlier history. Batch-mass notes do not assert final concentrations; provider-declared stock rates remain qualified. No growth or identity equivalence claim inferred. |