Overview
- External ontology
- mediadive.medium:J19 (source link unavailable) — BIFIDOBACTERIUM MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 6.8
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=19
Composition
flowchart LR
CultureMech_002562["bifidobacterium_medium"]
ing0_Casein_peptone["Casein peptone"]
CultureMech_002562 --> ing0_Casein_peptone
ing1_Beef_extract["Beef extract"]
CultureMech_002562 --> ing1_Beef_extract
ing2_Yeast_extract["Yeast extract"]
CultureMech_002562 --> ing2_Yeast_extract
ing3_Glucose["Glucose"]
CultureMech_002562 --> ing3_Glucose
CHEBI_17234["CHEBI:17234"]
ing3_Glucose -.-> CHEBI_17234
ing4_K2HPO4["K2HPO4"]
CultureMech_002562 --> ing4_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing4_K2HPO4 -.-> CHEBI_131527
ing5_Tween_80["Tween 80"]
CultureMech_002562 --> ing5_Tween_80
CHEBI_53426["CHEBI:53426"]
ing5_Tween_80 -.-> CHEBI_53426
Ingredients (6)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Casein peptone | 9.99001 G_PER_L | ( tryptic digest) | |
| 2 | Beef extract | 4.99501 G_PER_L | ||
| 3 | Yeast extract | 4.99501 G_PER_L | ||
| 4 |
Glucose
C6H12O6 180.156 g/mol
|
9.99001 G_PER_L |
CHEBI:17234
glucose
|
|
| 5 |
K2HPO4
HO4P.2K 174.174 g/mol
|
2.997 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 6 |
Tween 80
(C2H4O)w.(C2H4O)x.(C2H4O)y.(C2H4O)z.C24H44O6 604.822 g/mol
|
1 G_PER_L |
CHEBI:53426
polysorbate 80
|
Preparation
- Adjust pH to 6.8.
- After sterilization, aseptically add solutions of sodium ascorbate and L-cysteine x HCl to final concentration of 1.0% and 0.05%, respectively. Heat medium not freshly prepared in a steamer for 10 min before addition of the reducing substances.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:15.593283Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J19 |
| 2026-03-13T06:19:14.181687Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002562 |
| 2026-03-17T06:30:22.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:27.395583+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "BIFIDOBACTERIUM MEDIUM" to "bifidobacterium_medium" for consistent matching |
| 2026-06-05T07:40:16.818683+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 3 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:42.831072+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |