bsk_medium

CultureMech:002564 bacterial COMPLEX LIQUID
Original name: BSK MEDIUM

Overview

External ontology
mediadive.medium:J201 (source link unavailable) — BSK MEDIUM
Applications
  • Microbial cultivation
pH
7.6
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=201 Superseded historical provenance (not a current formulation claim): Commercial Product: LB Medium (Luria-Bertani, Miller formulation) Supplier: Multiple suppliers (Difco, Sigma, etc.) Catalog: Multiple (L3522 Sigma, 244520 BD) Source: https://www.laboratorynotes.com/preparation-of-luria-bertani-lb-miller-broth/ Accessed: 2026-03-28 Constituent composition researched from product specification. The exact source specifies albumin fraction V, not LB broth. Remove only the reviewed LB supplier nodes and restore the source material at its preparation stage without inventing chemical grounding. The linked original JCM201 endpoint returned no recipe in the pinned capture. Formulation assertions here are provider-backed, not original-page corroborated.

Composition

flowchart LR
    CultureMech_002564["bsk_medium"]
    ing0_Neopeptone["Neopeptone"]
    CultureMech_002564 --> ing0_Neopeptone
    ing1_Bovine_albumin["Bovine albumin"]
    CultureMech_002564 --> ing1_Bovine_albumin
    ing2_HEPES["HEPES"]
    CultureMech_002564 --> ing2_HEPES
    CHEBI_46756["CHEBI:46756"]
    ing2_HEPES -.-> CHEBI_46756
    ing3_Trisodium_citrate["Trisodium citrate"]
    CultureMech_002564 --> ing3_Trisodium_citrate
    CHEBI_53258["CHEBI:53258"]
    ing3_Trisodium_citrate -.-> CHEBI_53258
    ing4_Glucose["Glucose"]
    CultureMech_002564 --> ing4_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing4_Glucose -.-> CHEBI_17234
    ing5_Sodium_pyruvate["Sodium pyruvate"]
    CultureMech_002564 --> ing5_Sodium_pyruvate
    CHEBI_50144["CHEBI:50144"]
    ing5_Sodium_pyruvate -.-> CHEBI_50144
    ing6_N_Acetyl_D_glucosamine["N-Acetyl-D-glucosamine"]
    CultureMech_002564 --> ing6_N_Acetyl_D_glucosamine
    CHEBI_506227["CHEBI:506227"]
    ing6_N_Acetyl_D_glucosamine -.-> CHEBI_506227
    ing7_NaHCO3["NaHCO3"]
    CultureMech_002564 --> ing7_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing7_NaHCO3 -.-> CHEBI_32139
    ing8_Distilled_water["Distilled water"]
    CultureMech_002564 --> ing8_Distilled_water

Ingredients (9)

# Ingredient Concentration Ontology Notes
1 Neopeptone Source batch input: 5 g. Final preparation volume is not independently established; the unsupported g/L assertion is withdrawn, not replaced by an assumed one-liter concentration. Source qualifier: BD-Difco.
2 Bovine albumin Source batch input: 50 g. Final preparation volume is not independently established; the source batch amount is retained without asserting a final concentration. Source material qualifier: Fraction V.
3 HEPES CHEBI:46756
HEPES
 Source batch input: 6 g. Final preparation volume is not independently established; the unsupported g/L assertion is withdrawn, not replaced by an assumed one-liter concentration.
4 Trisodium citrate
C6H5O7.3Na 258.069 g/mol
CHEBI:53258
sodium citrate
 Source batch input: 0.7 g. Final preparation volume is not independently established; the unsupported g/L assertion is withdrawn, not replaced by an assumed one-liter concentration.
5 Glucose
C6H12O6 180.156 g/mol
CHEBI:17234
glucose
 Source batch input: 5 g. Final preparation volume is not independently established; the unsupported g/L assertion is withdrawn, not replaced by an assumed one-liter concentration.
6 Sodium pyruvate
C3H3O3.Na 110.044 g/mol
CHEBI:50144
sodium pyruvate
 Source batch input: 0.8 g. Final preparation volume is not independently established; the unsupported g/L assertion is withdrawn, not replaced by an assumed one-liter concentration.
7 N-Acetyl-D-glucosamine
C8H15NO6 221.209 g/mol
CHEBI:506227
N-acetyl-D-glucosamine
 Source batch input: 0.4 g. Final preparation volume is not independently established; the unsupported g/L assertion is withdrawn, not replaced by an assumed one-liter concentration.
8 NaHCO3
CHO3.Na 84.006 g/mol
CHEBI:32139
sodium hydrogencarbonate
 Source batch input: 2.2 g. Final preparation volume is not independently established; the unsupported g/L assertion is withdrawn, not replaced by an assumed one-liter concentration.
9 Distilled water 0.9 L Source water batch input: 900 ml = 0.9 L. This is not a measured final volume.

Preparation

  1. Mix all ingredients in a beaker with a stir bar. Adjust pH to 7.6. Add 200 ml of 7% gelatin. Filter-sterilize entire medium. Add sterile rabbit serum to final concentration of 6%.

Solutions

CMRL 1066

Dose: 0.1 L

Source preparation input: 100 ml = 0.1 L per receiving batch, not grams per liter or a dose per final liter. Source product qualifier: Gibco 330-1540. The source does not provide this product's composition or numeric stock strength.

7% gelatin solution

Dose: 0.2 L

Stock composition
IngredientConcentrationNotes
Gelatin Source label: 7% gelatin. Percentage basis is unspecified; no numeric stock strength or stock-batch mass is asserted.
Source late preparation input: 200 ml = 0.2 L of 7% gelatin per receiving batch, added before filtration of the entire medium. Percentage basis and stock mass/solvent recipe are unspecified.

Sterile rabbit serum

Source specifies sterile rabbit serum at final 6%, but the percentage basis and corresponding fixed addition volume are unspecified. Retain the literal label without asserting a numeric percentage, stock strength or dose. Serum is added after filtration of the medium.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:15.948222Z mediadive-import Imported from MediaDive Source: JCM, ID: J201
2026-03-13T06:19:14.188327Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002564
2026-03-17T06:30:05.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:27.403139+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "BSK MEDIUM" to "bsk_medium" for consistent matching
2026-06-05T07:40:16.825876+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-10-08T21:15:01.038694+00:00 codex RESTORED_JCM_ALBUMIN_IDENTITIES_AND_REAGENT_INPUTS_WITH_EVIDENCE Removed false LB expansions and restored albumin fraction V, source batch inputs, reagent doses and late preparations with exact evidence. Unknown percentage bases, final volumes, stock strengths and chemical groundings remain unresolved. The JCM201 original-source access gap is disclosed, not treated as corroboration.