pyrodictium_brockii_medium

CultureMech:002565 archaea COMPLEX LIQUID
Original name: PYRODICTIUM BROCKII MEDIUM

Overview

External ontology
mediadive.medium:J203 (source link unavailable) — PYRODICTIUM BROCKII MEDIUM
Applications
  • Microbial cultivation
pH
5.5
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=203

Composition

flowchart LR
    CultureMech_002565["pyrodictium_brockii_medium"]
    ing0_Citric_acid["Citric acid"]
    CultureMech_002565 --> ing0_Citric_acid
    CHEBI_30769["CHEBI:30769"]
    ing0_Citric_acid -.-> CHEBI_30769
    ing1_Yeast_extract["Yeast extract"]
    CultureMech_002565 --> ing1_Yeast_extract
    ing2_Sulfur["Sulfur"]
    CultureMech_002565 --> ing2_Sulfur
    CHEBI_26833["CHEBI:26833"]
    ing2_Sulfur -.-> CHEBI_26833
    ing3_Resazurin["Resazurin"]
    CultureMech_002565 --> ing3_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing3_Resazurin -.-> CHEBI_8806
    ing4_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_002565 --> ing4_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing4_Na2S_x_9_H2O -.-> CHEBI_76209
    ing5_NaCl["NaCl"]
    CultureMech_002565 --> ing5_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing5_NaCl -.-> CHEBI_26710
    ing6_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_002565 --> ing6_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing6_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing7_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_002565 --> ing7_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing7_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing8_KCl["KCl"]
    CultureMech_002565 --> ing8_KCl
    CHEBI_32588["CHEBI:32588"]
    ing8_KCl -.-> CHEBI_32588
    ing9_NaBr["NaBr"]
    CultureMech_002565 --> ing9_NaBr
    CHEBI_63004["CHEBI:63004"]
    ing9_NaBr -.-> CHEBI_63004
    ing10_H3BO3["H3BO3"]
    CultureMech_002565 --> ing10_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing10_H3BO3 -.-> CHEBI_33118
    ing11_SrCl2_x_6_H2O["SrCl2 x 6 H2O"]
    CultureMech_002565 --> ing11_SrCl2_x_6_H2O
    CHEBI_36385["CHEBI:36385"]
    ing11_SrCl2_x_6_H2O -.-> CHEBI_36385
    ing12_KI["KI"]
    CultureMech_002565 --> ing12_KI
    CHEBI_8346["CHEBI:8346"]
    ing12_KI -.-> CHEBI_8346
    ing13_CaCl2["CaCl2"]
    CultureMech_002565 --> ing13_CaCl2
    CHEBI_3312["CHEBI:3312"]
    ing13_CaCl2 -.-> CHEBI_3312
    ing14_KH2PO4["KH2PO4"]
    CultureMech_002565 --> ing14_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing14_KH2PO4 -.-> CHEBI_63036
    ing15__NH4_2Ni_SO4_2_x_6_H2O["(NH4)2Ni(SO4)2 x 6 H2O"]
    CultureMech_002565 --> ing15__NH4_2Ni_SO4_2_x_6_H2O
    CHEBI_86149["CHEBI:86149"]
    ing15__NH4_2Ni_SO4_2_x_6_H2O -.-> CHEBI_86149
    ing16_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
    CultureMech_002565 --> ing16_Nitrilotriacetic_acid
    CHEBI_44557["CHEBI:44557"]
    ing16_Nitrilotriacetic_acid -.-> CHEBI_44557
    ing17_MnSO4_x_n_H2O["MnSO4 x n H2O"]
    CultureMech_002565 --> ing17_MnSO4_x_n_H2O
    CHEBI_86360["CHEBI:86360"]
    ing17_MnSO4_x_n_H2O -.-> CHEBI_86360
    ing18_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_002565 --> ing18_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing18_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing19_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
    CultureMech_002565 --> ing19_CoSO4_x_7_H2O
    CHEBI_91244["CHEBI:91244"]
    ing19_CoSO4_x_7_H2O -.-> CHEBI_91244
    ing20_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_002565 --> ing20_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing20_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing21_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_002565 --> ing21_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing21_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing22_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_002565 --> ing22_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing22_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing23_AlK_SO4_2["AlK(SO4)2"]
    CultureMech_002565 --> ing23_AlK_SO4_2
    CHEBI_86463["CHEBI:86463"]
    ing23_AlK_SO4_2 -.-> CHEBI_86463
    ing24_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_002565 --> ing24_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing24_Na2MoO4_x_2_H2O -.-> CHEBI_75213

Ingredients (25)

# Ingredient Concentration Ontology Notes
1 Citric acid
C6H8O7 192.123 g/mol
0.00490196 G_PER_L CHEBI:30769
citric acid
2 Yeast extract 1.96078 G_PER_L
3 Sulfur
S 32.067 g/mol
29.4118 G_PER_L CHEBI:26833
sulfur atom
4 Resazurin
C12H7NO4 229.191 g/mol
0.000980392 G_PER_L CHEBI:8806
Resazurin
5 Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
10 G_PER_L CHEBI:76209
sodium sulfide nonahydrate
6 NaCl
Cl.Na 58.443 g/mol
14.85 G_PER_L CHEBI:26710
sodium chloride
 [Merged 2 duplicates: 13.85, 1.0]
7 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
6.5 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
 [Merged 2 duplicates: 3.5, 3.0]
8 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
2.75 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
9 KCl
Cl.K 74.551 g/mol
0.325 G_PER_L CHEBI:32588
potassium chloride
10 NaBr
Br.Na 102.894 g/mol
0.05 G_PER_L CHEBI:63004
sodium bromide
11 H3BO3
H3BO3 61.833 g/mol
0.025 G_PER_L CHEBI:33118
boric acid
 [Merged 2 duplicates: 0.015, 0.01]
12 SrCl2 x 6 H2O
Cl2Sr.6H2O 266.616 g/mol
0.0075 G_PER_L CHEBI:36385
strontium dichloride hexahydrate
13 KI
I.K 166.002 g/mol
5e-05 G_PER_L CHEBI:8346
potassium iodide
14 CaCl2
Ca.2Cl 110.984 g/mol
0.75 G_PER_L CHEBI:3312
calcium dichloride
15 KH2PO4
H2O4P.K 136.084 g/mol
0.5 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
16 (NH4)2Ni(SO4)2 x 6 H2O
6H2O.2H4N.Ni.2O4S 394.987 g/mol
0.002 G_PER_L CHEBI:86149
ammonium nickel sulfate hexahydrate
17 Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
1.5 G_PER_L CHEBI:44557
nitrilotriacetic acid
18 MnSO4 x n H2O
Mn.O4S 151.001 g/mol
0.5 G_PER_L CHEBI:86360
manganese(II) sulfate
19 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.1 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
20 CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
0.1 G_PER_L CHEBI:91244
cobalt(2+) sulfate heptahydrate
21 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.1 G_PER_L CHEBI:86158
calcium chloride dihydrate
22 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.1 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
23 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
0.01 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
24 AlK(SO4)2
Al.K.2O4S 258.206 g/mol
0.01 G_PER_L CHEBI:86463
potassium aluminium sulfate
25 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.01 G_PER_L CHEBI:75213
sodium molybdate dihydrate

Preparation

  1. Mix components except sulfur, adjust pH to 5.5 with H2SO4 and autoclave under a N2 gas atmosphere. Steam sulfur for 3 hr on each of 3 successive days. Aseptically distribute the medium and sulfur into culture vessels (e.g., 20 ml of the medium and 0.6 g sulfur in 120 ml serum bottles) under a stream of a H2-CO4 (80:20, v/v) gas mixture and seal with butyl rubber stoppers. Prior to inoculation, add per litter the following solution (autoclave and stored under a N2 atmosphere):
  2. Readjust pH to 5.5, if necessary. Pressurize inoculated vessels to 200kPa H2 -CO2 (80:20, v/v).
  3. Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:15.951577Z mediadive-import Imported from MediaDive Source: JCM, ID: J203
2026-03-13T03:09:40.789249Z data-quality-cleanup-v1.0 Data quality fixes Merged 3 duplicate ingredient(s)
2026-03-13T06:19:14.198031Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002565
2026-03-17T06:30:53.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:27.413742+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "PYRODICTIUM BROCKII MEDIUM" to "pyrodictium_brockii_medium" for consistent matching
2026-06-05T07:41:43.378076+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 22 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:10.695655+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.