Overview
- External ontology
- mediadive.medium:J209 (source link unavailable) — M98-5
- Applications
-
- Microbial cultivation
- pH
- 6.8
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=209
Composition
flowchart LR
CultureMech_002570["m98_5"]
ing0_Rumen_fluid["Rumen fluid"]
CultureMech_002570 --> ing0_Rumen_fluid
ing1_Trypticase_peptone["Trypticase peptone"]
CultureMech_002570 --> ing1_Trypticase_peptone
ing2_Yeast_extract["Yeast extract"]
CultureMech_002570 --> ing2_Yeast_extract
ing3_Glucose["Glucose"]
CultureMech_002570 --> ing3_Glucose
CHEBI_17234["CHEBI:17234"]
ing3_Glucose -.-> CHEBI_17234
ing4_Cellobiose["Cellobiose"]
CultureMech_002570 --> ing4_Cellobiose
CHEBI_17057["CHEBI:17057"]
ing4_Cellobiose -.-> CHEBI_17057
ing5_Maltose["Maltose"]
CultureMech_002570 --> ing5_Maltose
CHEBI_17306["CHEBI:17306"]
ing5_Maltose -.-> CHEBI_17306
ing6_Glycerol["Glycerol"]
CultureMech_002570 --> ing6_Glycerol
CHEBI_17754["CHEBI:17754"]
ing6_Glycerol -.-> CHEBI_17754
ing7_Agar["Agar"]
CultureMech_002570 --> ing7_Agar
CHEBI_2509["CHEBI:2509"]
ing7_Agar -.-> CHEBI_2509
ing8_Hemin["Hemin"]
CultureMech_002570 --> ing8_Hemin
CHEBI_50385["CHEBI:50385"]
ing8_Hemin -.-> CHEBI_50385
ing9_Resazurin["Resazurin"]
CultureMech_002570 --> ing9_Resazurin
CHEBI_8806["CHEBI:8806"]
ing9_Resazurin -.-> CHEBI_8806
ing10_Na2CO3_CO2_buffer["Na2CO3-CO2 buffer"]
CultureMech_002570 --> ing10_Na2CO3_CO2_buffer
CHEBI_29377["CHEBI:29377"]
ing10_Na2CO3_CO2_buffer -.-> CHEBI_29377
ing11_L_Ascorbic_acid["L-Ascorbic acid"]
CultureMech_002570 --> ing11_L_Ascorbic_acid
CHEBI_29073["CHEBI:29073"]
ing11_L_Ascorbic_acid -.-> CHEBI_29073
ing12_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
CultureMech_002570 --> ing12_L_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing12_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
ing13_K2HPO4["K2HPO4"]
CultureMech_002570 --> ing13_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing13_K2HPO4 -.-> CHEBI_131527
ing14_KH2PO4["KH2PO4"]
CultureMech_002570 --> ing14_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing14_KH2PO4 -.-> CHEBI_63036
ing15_NaCl["NaCl"]
CultureMech_002570 --> ing15_NaCl
CHEBI_26710["CHEBI:26710"]
ing15_NaCl -.-> CHEBI_26710
ing16__NH4_2SO4["(NH4)2SO4"]
CultureMech_002570 --> ing16__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing16__NH4_2SO4 -.-> CHEBI_62946
ing17_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_002570 --> ing17_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing17_CaCl2_x_2_H2O -.-> CHEBI_86158
ing18_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_002570 --> ing18_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing18_MgSO4_x_7_H2O -.-> CHEBI_31795
Ingredients (19)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Rumen fluid | 400 G_PER_L | ||
| 2 | Trypticase peptone | 1.8315 G_PER_L | ||
| 3 | Yeast extract | 0.457875 G_PER_L | ||
| 4 |
Glucose
C6H12O6 180.156 g/mol
|
0.274725 G_PER_L |
CHEBI:17234
glucose
|
|
| 5 |
Cellobiose
C12H22O11 342.297 g/mol
|
0.457875 G_PER_L |
CHEBI:17057
cellobiose
|
|
| 6 |
Maltose
C12H22O11 342.297 g/mol
|
0.274725 G_PER_L |
CHEBI:17306
maltose
|
|
| 7 |
Glycerol
C3H8O3 92.094 g/mol
|
0.274725 G_PER_L |
CHEBI:17754
glycerol
|
|
| 8 | Agar | 18.315 G_PER_L |
CHEBI:2509
agar
|
|
| 9 |
Hemin
C34H32ClFeN4O4 651.952 g/mol
|
1 G_PER_L |
CHEBI:50385
hemin
|
|
| 10 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.5 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 11 |
Na2CO3-CO2 buffer
CO3.2Na 105.988 g/mol
|
100 G_PER_L |
CHEBI:29377
sodium carbonate
|
|
| 12 |
L-Ascorbic acid
C6H8O6 176.124 g/mol
|
1 G_PER_L |
CHEBI:29073
L-ascorbic acid
|
|
| 13 |
L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
10 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
|
| 14 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.714286 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 15 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.430403 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 16 |
NaCl
Cl.Na 58.443 g/mol
|
1.08059 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 17 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
1.0989 G_PER_L |
CHEBI:62946
ammonium sulfate
|
|
| 18 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.10989 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 19 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.228938 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Preparation
- <I>Salts solution</I>
- Adjust pH to 6.7-6.8.
Solutions
Salts solution
Dose: 50 G_PER_L
See mediadive_4429_Salts_solution.yaml for composition
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:15.964702Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J209 |
| 2026-03-09T08:04:55.565123+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 1 solution(s) from ingredients |
| 2026-03-13T06:19:14.223874Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002570 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name |
| 2026-03-17T06:30:36.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:27.443036+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "M98-5" to "m98_5" for consistent matching |
| 2026-05-16T09:03:39.543292+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:41:11.909304+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 16 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:00.710881+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |