jcm_medium_no_212

CultureMech:002574 bacterial COMPLEX SOLID_AGAR
Original name: JCM MEDIUM No. 212

Overview

External ontology
mediadive.medium:J212 (source link unavailable) — JCM MEDIUM No. 212
Applications
  • Microbial cultivation
pH
7.0
High-metal flag
True
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=212

Composition

flowchart LR
    CultureMech_002574["jcm_medium_no_212"]
    ing0_NaCl["NaCl"]
    CultureMech_002574 --> ing0_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing0_NaCl -.-> CHEBI_26710
    ing1_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_002574 --> ing1_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing1_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_002574 --> ing2_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing3_KCl["KCl"]
    CultureMech_002574 --> ing3_KCl
    CHEBI_32588["CHEBI:32588"]
    ing3_KCl -.-> CHEBI_32588
    ing4_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_002574 --> ing4_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing4_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing5_NaHCO3["NaHCO3"]
    CultureMech_002574 --> ing5_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing5_NaHCO3 -.-> CHEBI_32139
    ing6_KBr["KBr"]
    CultureMech_002574 --> ing6_KBr
    CHEBI_32030["CHEBI:32030"]
    ing6_KBr -.-> CHEBI_32030
    ing7_Yeast_extract["Yeast extract"]
    CultureMech_002574 --> ing7_Yeast_extract
    ing8_Glucose["Glucose"]
    CultureMech_002574 --> ing8_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing8_Glucose -.-> CHEBI_17234
    ing9_Agar["Agar"]
    CultureMech_002574 --> ing9_Agar
    CHEBI_2509["CHEBI:2509"]
    ing9_Agar -.-> CHEBI_2509

Ingredients (10)

# Ingredient Concentration Ontology Notes
1 NaCl
Cl.Na 58.443 g/mol
200 G_PER_L CHEBI:26710
sodium chloride
2 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
13 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
3 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
20 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
4 KCl
Cl.K 74.551 g/mol
4 G_PER_L CHEBI:32588
potassium chloride
5 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
1 G_PER_L CHEBI:86158
calcium chloride dihydrate
6 NaHCO3
CHO3.Na 84.006 g/mol
0.2 G_PER_L CHEBI:32139
sodium hydrogencarbonate
7 KBr
Br.K 119.002 g/mol
0.5 G_PER_L CHEBI:32030
potassium bromide
8 Yeast extract 5 G_PER_L
9 Glucose
C6H12O6 180.156 g/mol
1 G_PER_L CHEBI:17234
glucose
10 Agar 20 G_PER_L CHEBI:2509
agar

Preparation

  1. Add components to distilled water and bring volume to 1.0 L.
  2. Adjust pH to 7.0.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:15.971368Z mediadive-import Imported from MediaDive Source: JCM, ID: J212
2026-03-13T06:19:14.239993Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002574
2026-03-17T06:30:57.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:27.461108+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "JCM MEDIUM No. 212" to "jcm_medium_no_212" for consistent matching
2026-06-05T07:41:09.926413+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 9 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:00.224888+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.