Overview
- External ontology
- mediadive.medium:J216 (source link unavailable) — JCM MEDIUM No. 216
- Applications
-
- Microbial cultivation
- pH
- 6.0
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=216
Composition
flowchart LR
CultureMech_002578["jcm_medium_no_216"]
ing0_NH4Cl["NH4Cl"]
CultureMech_002578 --> ing0_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing0_NH4Cl -.-> CHEBI_31206
ing1_K2HPO4["K2HPO4"]
CultureMech_002578 --> ing1_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing1_K2HPO4 -.-> CHEBI_131527
ing2_KH2PO4["KH2PO4"]
CultureMech_002578 --> ing2_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing2_KH2PO4 -.-> CHEBI_63036
ing3_NaCl["NaCl"]
CultureMech_002578 --> ing3_NaCl
CHEBI_26710["CHEBI:26710"]
ing3_NaCl -.-> CHEBI_26710
ing4_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_002578 --> ing4_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing4_MgSO4_x_7_H2O -.-> CHEBI_31795
ing5_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_002578 --> ing5_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing5_MgCl2_x_6_H2O -.-> CHEBI_86345
ing6_Na2SO4["Na2SO4"]
CultureMech_002578 --> ing6_Na2SO4
CHEBI_32149["CHEBI:32149"]
ing6_Na2SO4 -.-> CHEBI_32149
ing7_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_002578 --> ing7_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing7_CaCl2_x_2_H2O -.-> CHEBI_86158
ing8_KCl["KCl"]
CultureMech_002578 --> ing8_KCl
CHEBI_32588["CHEBI:32588"]
ing8_KCl -.-> CHEBI_32588
ing9_NaHCO3["NaHCO3"]
CultureMech_002578 --> ing9_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing9_NaHCO3 -.-> CHEBI_32139
ing10_Fe_NH4_2_SO4_2_x_6_H2O["Fe(NH4)2(SO4)2 x 6 H2O"]
CultureMech_002578 --> ing10_Fe_NH4_2_SO4_2_x_6_H2O
CHEBI_76181["CHEBI:76181"]
ing10_Fe_NH4_2_SO4_2_x_6_H2O -.-> CHEBI_76181
ing11__NH4_2Ni_SO4_2_x_6_H2O["(NH4)2Ni(SO4)2 x 6 H2O"]
CultureMech_002578 --> ing11__NH4_2Ni_SO4_2_x_6_H2O
CHEBI_86149["CHEBI:86149"]
ing11__NH4_2Ni_SO4_2_x_6_H2O -.-> CHEBI_86149
ing12_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
CultureMech_002578 --> ing12_Na2WO4_x_2_H2O
CHEBI_63939["CHEBI:63939"]
ing12_Na2WO4_x_2_H2O -.-> CHEBI_63939
ing13_Resazurin["Resazurin"]
CultureMech_002578 --> ing13_Resazurin
CHEBI_8806["CHEBI:8806"]
ing13_Resazurin -.-> CHEBI_8806
ing14_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_002578 --> ing14_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing14_Na2S_x_9_H2O -.-> CHEBI_76209
ing15_NaBr["NaBr"]
CultureMech_002578 --> ing15_NaBr
CHEBI_63004["CHEBI:63004"]
ing15_NaBr -.-> CHEBI_63004
ing16_SrCl2_x_6_H2O["SrCl2 x 6 H2O"]
CultureMech_002578 --> ing16_SrCl2_x_6_H2O
CHEBI_36385["CHEBI:36385"]
ing16_SrCl2_x_6_H2O -.-> CHEBI_36385
ing17_H3BO3["H3BO3"]
CultureMech_002578 --> ing17_H3BO3
CHEBI_33118["CHEBI:33118"]
ing17_H3BO3 -.-> CHEBI_33118
ing18_KI["KI"]
CultureMech_002578 --> ing18_KI
CHEBI_8346["CHEBI:8346"]
ing18_KI -.-> CHEBI_8346
ing19_Sodium_silicate["Sodium silicate"]
CultureMech_002578 --> ing19_Sodium_silicate
CHEBI_60720["CHEBI:60720"]
ing19_Sodium_silicate -.-> CHEBI_60720
ing20_NaF["NaF"]
CultureMech_002578 --> ing20_NaF
CHEBI_28741["CHEBI:28741"]
ing20_NaF -.-> CHEBI_28741
ing21_KNO3["KNO3"]
CultureMech_002578 --> ing21_KNO3
CHEBI_63043["CHEBI:63043"]
ing21_KNO3 -.-> CHEBI_63043
ing22_Na2HPO4_x_12_H2O["Na2HPO4 x 12 H2O"]
CultureMech_002578 --> ing22_Na2HPO4_x_12_H2O
CHEBI_34683["CHEBI:34683"]
ing22_Na2HPO4_x_12_H2O -.-> CHEBI_34683
ing23_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_002578 --> ing23_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing23_Nitrilotriacetic_acid -.-> CHEBI_44557
ing24_MnSO4_x_n_H2O["MnSO4 x n H2O"]
CultureMech_002578 --> ing24_MnSO4_x_n_H2O
CHEBI_86360["CHEBI:86360"]
ing24_MnSO4_x_n_H2O -.-> CHEBI_86360
ing25_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_002578 --> ing25_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing25_FeSO4_x_7_H2O -.-> CHEBI_75836
ing26_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
CultureMech_002578 --> ing26_CoSO4_x_7_H2O
CHEBI_91244["CHEBI:91244"]
ing26_CoSO4_x_7_H2O -.-> CHEBI_91244
ing27_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_002578 --> ing27_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing27_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing28_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_002578 --> ing28_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing28_CuSO4_x_5_H2O -.-> CHEBI_31440
ing29_AlK_SO4_2["AlK(SO4)2"]
CultureMech_002578 --> ing29_AlK_SO4_2
CHEBI_86463["CHEBI:86463"]
ing29_AlK_SO4_2 -.-> CHEBI_86463
CultureMech_002578 --> more["...11 more"]
Ingredients (41)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.25 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 2 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.05 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 3 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.09 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 4 |
NaCl
Cl.Na 58.443 g/mol
|
12.8 G_PER_L |
CHEBI:26710
sodium chloride
|
[Merged 2 duplicates: 11.8, 1.0] |
| 5 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
4.75 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
[Merged 2 duplicates: 1.75, 3.0] |
| 6 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
4.5 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 7 |
Na2SO4
2Na.O4S 142.043 g/mol
|
0.81 G_PER_L |
CHEBI:32149
sodium sulfate
|
|
| 8 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.88 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
[Merged 2 duplicates: 0.78, 0.1] |
| 9 |
KCl
Cl.K 74.551 g/mol
|
0.3 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 10 |
NaHCO3
CHO3.Na 84.006 g/mol
|
1 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 11 |
Fe(NH4)2(SO4)2 x 6 H2O
Fe.6H2O.2H4N.2O4S 392.139 g/mol
|
0.002 G_PER_L |
CHEBI:76181
ferrous ammonium sulfate hexahydrate
|
|
| 12 |
(NH4)2Ni(SO4)2 x 6 H2O
6H2O.2H4N.Ni.2O4S 394.987 g/mol
|
0.002 G_PER_L |
CHEBI:86149
ammonium nickel sulfate hexahydrate
|
|
| 13 |
Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.002 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
|
| 14 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.001 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 15 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
0.5 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
|
| 16 |
NaBr
Br.Na 102.894 g/mol
|
4 G_PER_L |
CHEBI:63004
sodium bromide
|
|
| 17 |
SrCl2 x 6 H2O
Cl2Sr.6H2O 266.616 g/mol
|
1.8 G_PER_L |
CHEBI:36385
strontium dichloride hexahydrate
|
|
| 18 |
H3BO3
H3BO3 61.833 g/mol
|
1.31 G_PER_L |
CHEBI:33118
boric acid
|
[Merged 2 duplicates: 1.3, 0.01] |
| 19 |
KI
I.K 166.002 g/mol
|
0.00125 G_PER_L |
CHEBI:8346
potassium iodide
|
|
| 20 |
Sodium silicate
2Na.O3Si 122.063 g/mol
|
0.1 G_PER_L |
CHEBI:60720
sodium silicate
|
|
| 21 |
NaF
F.Na 41.988 g/mol
|
0.06 G_PER_L |
CHEBI:28741
sodium fluoride
|
|
| 22 |
KNO3
K.NO3 101.102 g/mol
|
0.04 G_PER_L |
CHEBI:63043
potassium nitrate
|
|
| 23 |
Na2HPO4 x 12 H2O
HO4P.2Na 141.958 g/mol
|
0.46 G_PER_L |
CHEBI:34683
disodium hydrogenphosphate
|
|
| 24 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
1.5 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
|
| 25 |
MnSO4 x n H2O
Mn.O4S 151.001 g/mol
|
0.5 G_PER_L |
CHEBI:86360
manganese(II) sulfate
|
|
| 26 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.1 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 27 |
CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
|
0.1 G_PER_L |
CHEBI:91244
cobalt(2+) sulfate heptahydrate
|
|
| 28 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.1 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 29 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.01 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 30 |
AlK(SO4)2
Al.K.2O4S 258.206 g/mol
|
0.01 G_PER_L |
CHEBI:86463
potassium aluminium sulfate
|
|
| 31 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.01 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 32 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.002 G_PER_L |
CHEBI:15956
biotin
|
|
| 33 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.002 G_PER_L |
CHEBI:27470
folic acid
|
|
| 34 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.01 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 35 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.005 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 36 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.005 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 37 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.005 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 38 |
Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.005 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 39 | Vitamin B12 | 0.0001 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 40 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.005 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 41 |
Lipoic acid
C8H14O2S2 206.332 g/mol
|
0.005 G_PER_L |
CHEBI:16494
lipoic acid
|
Preparation
- Mix ingredients, except Na2S x 9H2O, adjust pH to 6.0 with H2SO4. Sterilize by filtration while gassing with H2-CO2 (80:20) gas mixture. Autoclave Na2S x 9H2O as a 5% solution under a N2 atmosphere. Prior to inoculation, anaerobically add the sterile Na2S x 9H2O solution, and readjust pH to 6.5 with sterile H2SO4. Pressurize the inoculated bottle to 200 kPa H2-CO2 (80:20).
- Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:15.987346Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J216 |
| 2026-01-28T19:48:17.076759Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T03:08:57.020955Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 4 duplicate ingredient(s) |
| 2026-03-13T06:19:14.291378Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002578 |
| 2026-03-17T06:30:01.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:27.517625+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "JCM MEDIUM No. 216" to "jcm_medium_no_216" for consistent matching |
| 2026-06-05T07:41:09.942980+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 38 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:37:35.009016+00:00 | primary-term-regrounding-v1.0 | Re-grounded wrong primary term id (OAK-verified) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-06-10T07:47:00.227976+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 9 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |