Overview
- External ontology
- mediadive.medium:J219 (source link unavailable) — CYC AGAR (pH 8.0)
- Applications
-
- Microbial cultivation
- pH
- 8.0
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=219
Composition
flowchart LR
CultureMech_002580["cyc_agar_ph_8_0"]
ing0_Czapek_Dox_liquid_medium["Czapek-Dox liquid medium"]
CultureMech_002580 --> ing0_Czapek_Dox_liquid_medium
ing1_Yeast_extract["Yeast extract"]
CultureMech_002580 --> ing1_Yeast_extract
ing2_Casamino_acids["Casamino acids"]
CultureMech_002580 --> ing2_Casamino_acids
ing3_Agar["Agar"]
CultureMech_002580 --> ing3_Agar
CHEBI_2509["CHEBI:2509"]
ing3_Agar -.-> CHEBI_2509
Ingredients (4)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Czapek-Dox liquid medium | 33.4 G_PER_L | ( modified) | |
| 2 | Yeast extract | 2 G_PER_L |
FOODON:03315426
Yeast extract
|
|
| 3 | Casamino acids | 6 G_PER_L |
FOODON:03315719
Casamino acids
|
( vitamin assay) |
| 4 | Agar | 16 G_PER_L |
CHEBI:2509
agar
|
Preparation
- Adjust pH to 8.0.
- Adjust pH to 7.2.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:15.995973Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J219 |
| 2026-01-28T19:48:43.135838Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:19:14.302364Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002580 |
| 2026-03-17T06:30:57.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:27.530111+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "CYC AGAR (pH 8.0)" to "cyc_agar_ph_8_0" for consistent matching |
| 2026-06-05T07:40:55.143640+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 2 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:36.491340+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (audit G21/G22) | Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI). |
| 2026-09-13T00:00:00-07:00 | repair_bacterial_score10_exact_terms_batch2.py | GROUNDED_BACTERIAL_SCORE10_EXACT_TERMS_BATCH2 | Applied local mappings for Casamino acids, Yeast extract. |